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Long Non-Coding RNA LINC01783 Promotes the Progression of Cervical Cancer by Sponging miR-199b-5p to Mediate GBP1 Expression

Authors Chen W, Xiong L, Yang L, Yang L, Li L, Huang L, Liang X, Xue J, Tan B

Received 7 September 2019

Accepted for publication 1 November 2019

Published 16 January 2020 Volume 2020:12 Pages 363—373

DOI https://doi.org/10.2147/CMAR.S230171

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Eileen O'Reilly


Wei-jun Chen, 1,* Liang Xiong, 2,* Lin Yang, 3 Li-juan Yang, 3 Lin Li, 3 Li Huang, 3 Xiao-qing Liang, 4 Jie Xue, 3 Bu-zhen Tan 5

1Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Nanchang University, Reproductive Hospital Affiliated to Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People’s Republic of China; 2Department of Urinary Surgery, Armed Police Jiangxi General Team Hospital, Nanchang, Jiangxi, People’s Republic of China; 3Department of Reproductive Medicine, Reproductive Hospital Affiliated to Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People’s Republic of China; 4Department of Gynaecology and Obstetrics, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China; 5Department of Gynaecology and Obstetrics, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Bu-zhen Tan Email tanbuzhennc@sina.com

Background: Long non-coding RNA showed potential regulating effects in oncogenesis. Highly expressed LncRNA LINC01783 is observed in cervical cancer. However, the specific pathogenesis of cervical cancer is still unclear.
Methods: Differential lncRNAs in cervical cancer were identified based on TCGA dataset. Subsequently, qRT-PCR was utilized for testing the LINC01783 expression in cervical cancer cell lines and normal human cervical epithelial cell line HcerEpic. CCK-8, EdU, Wound healing assay, Transwell assay and flow cytometry were used for detecting proliferative and migratory potential, cell cycle and apoptosis of cervical cancer cells, respectively. To identify the potential target of LINC01783, bioinformatics assay and dual-luciferase reporter gene assay were performed. Moreover, to clarify their interactions and roles in regulating the progression of cervical cancer, Western blot assay and RIP assay were carried out.
Results: Our results revealed LINC01783 is overexpressed in cervical cancer cells. Overexpressed LINC01783 considerably accelerated the cell proliferation, migration and invasion of cervical cancer cells while restrained cell apoptosis of them. Moreover, LINC01783 positively regulated the GBP1 expression via competitively binding to miR-199b-5p.
Conclusion: LINC01783 is involved in the progression of cervical cancer through competitively binding to miR-199b-5p to mediate GBP1 expression.

Keywords: cervical cancer, LncRNA, ceRNA, proliferation, migration, invasion

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