Laser thermal ablation of multidrug-resistant bacteria using functionalized gold nanoparticles
Received 15 October 2016
Accepted for publication 23 December 2016
Published 23 March 2017 Volume 2017:12 Pages 2255—2263
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Thomas J. Webster
Lucian Mocan,1,2 Flaviu A Tabaran,3 Teodora Mocan,2,4 Teodora Pop,5 Ofelia Mosteanu,5 Lucia Agoston-Coldea,6 Cristian T Matea,2 Diana Gonciar,2 Claudiu Zdrehus,1,2 Cornel Iancu1
13rd Department of General Surgery, “Iuliu Hatieganu” University of Medicine and Pharmacy, 2Department of Nanomedicine, “Octavian Fodor” Gastroenterology Institute, 3Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 4Department of Physiology, 53rd Gastroenterology Department, 6Department of Internal Medicine, “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
Abstract: The issue of multidrug resistance (MDR) has become an increasing threat to public health. One alternative strategy against MDR bacteria would be to construct therapeutic vectors capable of physically damaging these microorganisms. Gold nanoparticles hold great promise for the development of such therapeutic agents, since the nanoparticles exhibit impressive properties, of which the most important is the ability to convert light into heat. This property has scientific significance since is exploited to develop nano-photothermal vectors to destroy bacteria at a molecular level. The present paper summarizes the latest advancements in the field of nanotargeted laser hyperthermia of MDR bacteria mediated by gold nanoparticles.
Keywords: bacteria, photo-thermal ablation, gold nanoparticles, antibiotic resistance
The problem of multidrug resistance (MDR) has become a growing threat to public health. A high number of infections and many deaths that occur each year in the USA are caused by bacteria that resist multiple antibiotics.1–7 Another issue is the rejection of certain drugs, a recent trend that has intensified the urgency of developing new therapeutic agents and approaches. Current studies are investigating the possible benefits of natural products, the changes that might be brought to certain classes of antibiotics in use today, and the production of host defense peptides.1,8–11 Due to their physical properties and potencies, nanoparticles have proven to be highly versatile and can be widely used in numerous applications.6,12–14 Their size and shape properties are compatible with various biomedical applications that require interaction with small-molecule antibiotics. The size of these nanomaterials provides a large surface-area-to-volume ratio, which enables the binding of a large number of high affinity ligands, allowing for nanoparticle multivalency in fighting bacteria. Based on their features, nanoparticles have been functionalized with antibiotics and used as antibacterial agents. Loading can be achieved by both covalent and noncovalent interactions. The resulting complexes have shown greater antibacterial effect and lower minimum inhibitory concentration than the antibiotics alone. This efficiency is the result of the polyvalent impact caused by the concentration of antibiotics on the surface of the nanoparticles, which is associated with a significant improvement in the amount of internalization of antibiotics by nanoparticles. The conjugation of functional ligands onto the surface of gold nanoparticles (GNPs) allows for direct multivalent interactions, which demonstrates the application of these nanomaterials as self-therapeutics. Such an approach could negate the drawbacks of the encapsulation of current antimicrobials. As they exhibit low toxicity and chemical inertia, GNPs have proven ideal for the assembly of self-therapeutics. GNPs have gained significant interest as nanovectors for therapy, representing a novel and competent substitute for effective transportation and translocation of therapeutic molecules.15–22 Various types of GNP complexes (protein-coated GNPs, peptide-coated GNPs, GNPs coated with nucleic acids, drugs) are available for delivery to cells and organs.
One of the most remarkable properties of GNPs is the ability to transform light into heat under laser irradiation.2,6,18,23–26 This property is significant because it can be exploited to develop nano-photothermal vectors in order to destroy bacteria at a molecular level. The present paper summarizes the latest advancements in the field of nanotargeted laser hyperthermia of MDR bacteria mediated by GNPs (Table 1).
Table 1 Antibacterial activity against multidrug-resistant bacteria correlated with the type of gold nanoparticles used
Nano-photothermal therapy mediated by GNPs in MDR Staphylococcus aureus
Light-absorbing GNPs have been functionalized with specific antibodies to achieve targeted and selective laser bacterial killing.27 This therapeutic intervention was assessed in real time using photothermal microscopy. The antibacterial effect was caused by the combination of strong overheating in the laser impact zone and the generation of bubbles around gold clusters. These results were confirmed by photothermal imaging and time-resolved imaging. The technology was further employed to selectively kill S. aureus by means of targeted delivery of 10, 20, and 40 nm GNPs functionalized with anti-protein A antibodies. Following exposure to laser pulses (420–570 nm, 12 ns, 0.1–5 J/cm2, 100 pulses), several techniques such as electron microscopy, optical transmission, and viability testing assessed laser killing of bacteria for different laser fluences and nanoparticle size ranges.27
In a study testing the antibacterial effect resulting from the association between antibody-targeted GNPs and pulsed laser irradiation,28 GNPs were functionalized with S. aureus-specific antibodies, followed by methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus incubation. Suspensions were further irradiated with a pulsed laser (8 ns, 532 nm, fluence range 1–5 J/cm2). The change in effect caused by this type of exposure was assessed at fluences ranging from 0 to 5 J/cm2. The results obtained in the group exposed to fluences of 0 J/cm2 showed a survival rate of 86%±20% (mean ± SD, n=6) compared to controls, with a decrease in survival from 75%±11% to 31%±8% in subjects exposed to fluences of 5 J/cm2. After exposure to 2, 3, and 5 J/cm2, there was a significant decrease in survival when compared to the first group, while differences were not as considerable for fluences of 1 J/cm2. Estimations made using regression analysis indicated evidence of a linear relationship between the destruction of drug-resistant bacteria by photothermal therapy and laser beam fluence (r2=0.97). The use of ligand-free nanoparticles or the absence of laser irradiation led to a considerably lower antibacterial effect (Figure 1).
Figure 1 Scanning electron microscope images of MSSA.
S. aureus was used as a proof-of-principle ESKAPE (Enterococcus faecium, S. aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogen in a recent study29 to show that the loading of the correct antibiotic, such as daptomycin, into the polydopamine coating based on gold nanocages ([email protected]) – where the strategy adopted for their synthesis was galvanic replacement – resulted in an outer edge length of 55±5 nm and an inner edge length of 38±5 nm. Another finding was that the conjugation of these complexes to antibodies targeting S. aureus surface protein A can selectively and directly deliver the nanoscale constructs onto the bacterial cell surfaces, as seen in Figure 1. The fact that there was no binding in mammalian cells helped confirm target specificity. The effect of 808 nm diode laser irradiation on [email protected] (200 μL, 0.04-0.4 nM) was represented by colony forming units (CFU) reduction below detection limits (20 CFU/well) at 0 hour. However, 24 hours after treatment, the cells were able to rebound above baseline. On the other hand, there was a decrease in bacterial cell viability below detection limits at 0 and 24 hours after laser irradiation of 4 μg/mL [email protected]/PDA (polydopamine-coated gold nanocages).29 There was also evidence of a decrease in the antibacterial effects of antibiotics in the presence of unconjugated aSpa depending on the concentration of [email protected]/PDA-aSpa nanoscale constructs. Therefore, the authors were able to confirm target specificity and greater synergistic antibacterial efficiency by means of bacterial cell surface localization of [email protected]/PDA–aSpa instead of the more abstract effects of microtiter plate-based antibacterial assays.
An agent that can detect MRSA and selectively destroy it when used in combination with photothermal therapy was developed in another study30 using gold nanorods (GNRs) and an anti-protein A antibody. The authors synthesized GNRs functionalized with polystyrene sulfonate, binding anti-protein A antibody to their surface. The preparation of the final complex was confirmed by Fourier transform infrared spectroscopy and ultraviolet–visible spectra. After the colonies were counted, the results indicated greater cell death (82%) in groups undergoing treatment with the experimental nanoconjugate in association with photothermal therapy than in others (P<0.05). After treatment with free GNRs combined with near-infrared (NIR) laser therapy, bacterial cell death (16%) was not significantly greater than in controls (P<0.05). Moreover, no cell death occurred when using NIR laser therapy alone. The extent to which the GNRs conjugated to anti-protein A antibody were absorbed in the vital organs of infected BALB/c mice at time intervals of 1, 4, 8, and 24 hours following intravenous inoculation was expressed as the percentage of injected dose per gram of organ (% ID/g). Outcomes revealed that the nanocomplex accumulated at the site of infection to a greater extent than in the respective healthy tissues and organs at all time intervals and especially, at 8 hours after inoculation (P<0.05). A considerable decrease in bacterial viability (73%) was observed in the in vivo murine models following photothermal therapy.30
GNRs conjugated to toluidine blue photosensitizer (Au-anionic poly(acrylic acid)-toluidine blue) showed its duality in a study conducted by Kuo et al on MRSA, determining both antimicrobial photodynamic inactivation and hyperthermia.31 Following treatment with the above-mentioned conjugate and sequential exposure to helium–neon laser (633 nm, 1 minute) and continuous wave laser (808 nm, 25 minutes), there was a significant reduction in MRSA viability (to about 5%) and a 10-fold increase in antimicrobial ability, compared to photodynamic antimicrobial chemotherapy or hyperthermia alone. There was no bacterial damage in the absence of nanorods after both types of laser therapies, but there was a decrease in viability to 59% and 53% following treatment with nanorods.31
Effective biofilm disinfection was obtained in another study32 using a GNR cluster that is easily excited by NIR laser irradiation, which generates heat rapidly through photothermal conversion. Wet chemistry was used for the synthesis of GNRs, and photolithography was used in the microfabrication of one GNR cluster. After being directly applied to the biofilm, the influence of the photothermal effect of the GNR cluster on the biofilm was assessed both prior to laser irradiation and after treatment, which showed a significant decrease in cell viability and biofilm thickness. As indicated by analysis of bacterial mortality rates and biofilm inactivation, greater efficacy was observed for longer exposures to laser irradiation. Clear morphologic damage was indicated by scanning electron microscopy images of bacteria following irradiation, including bacterial cell membrane rupture.
Core–shell gold–silver nanoparticles
Aspartame-stabilized gold–silver nanostructures ([email protected]@Asm) with core–shell architecture were developed in an attempt to associate the antimicrobial activity of silver with the thermosensitive properties of gold (Figure 2).33 Broth microdilution antibacterial assay was used to determine minimum inhibitory concentrations. The experiment was performed on strains of S. aureus subsp. aureus Rosenbach (ATCC 25923), Staphylococcus epidermidis SE19, and Escherichia coli CF073. Experiments on 120 identical plates kept in the dark or exposed to LED irradiation (530 nm) and stored at a temperature of 37°C for 18 hours were carried out in parallel to determine the optical density of the bacterial cultures. Experiments were performed with and without irradiation therapy to assess the antibacterial effect of [email protected]@Asm and the photothermal effect of gold. Results showed that bacterial killing was not only incomplete in the dark (even for below-detection values) but was also followed by regrowth. The opposite result was obtained following irradiation. Biocompatibility testing was performed using the MTS cell proliferation colorimetric assay, in which any significant effect on the proliferation of primary dermal fibroblasts following treatment with [email protected]@Asm at different concentrations was not observed.33
Figure 2 Gold–silver core–shell nanoparticles stabilized with aspartame proposed by Fasciani et al.33
In a recent study,34 monolayers of gold nanostars were synthesized by lauryl sulfobetaine-driven seeded growth method. The anti-biofilm activity was evaluated by preparing 32 round glass slides (1.0 cm in diameter) using localized surface plasmon resonance spectroscopy (802 nm, s =12) and setting the surface concentration of the adsorbate on the gold surface to 3.0 mg/cm2 (s =0.4). Experiments were carried out using a strong biofilm producer, an MRSA LP strain. Following laser irradiation, the conjugate exhibited an excellent photothermal conversion property in the NIR region. After exposure to 808 nm laser, the monolayers of gold nanostars generated a rise in tumor temperature (hyperthermia), which resulted in the efficient killing of the S. aureus biofilm.34
A class of shape- and size-selective antimicrobial agents was developed in a study conducted by Borovička et al.35 The experiment consisted of the fabrication of imprinted colloid particles using cell templates by the fragmentation of silica shells. The increased contact area between the target cell and the colloidal imprint led to the mechanism of recognition, which can help develop “colloid antibodies” for efficient shape-selective binding (Figure 3). GNPs were integrated into the silica shell of the colloid imprint to bind to matching yeast cells, which was followed by direct delivery onto the target cell surface. Laser irradiation of the cell dispersion prompted selective killing of the target cells. The impact of the photothermal colloid antibody on shape-selective recognition and killing of bacteria was also confirmed by a mixture of two differently shaped and differently sized types of bacterial cells.35
Figure 3 Proposed mechanism for shape-selective recognition and killing of microorganisms by Borovička et al.35
Nano-photothermal therapy mediated by GNPs in MDR P. aeruginosa
Covalent binding of primary antibodies to GNRs was used for the selective destruction of P. aeruginosa (Figure 4). The synthesis of nanorods for bacterial cell wall–targeted delivery was achieved by electrostatic or covalent binding of anti-PA3 primary antibodies to their surfaces. Bacteria were incubated with relevant antibody–nanorod complexes. Evidence of electron-dense GNR-covered bacteria was offered by transmission electron microscopy. Live and dead cell counts using the Live/Dead kit (Invitrogen, Carlsbad, CA, USA) revealed up to 80% cell viability in the case of PA3 cells without the attachment of nanorods or NIR irradiation, NIR-irradiated cells without the attachment of nanorods, and bacteria with attached nanorods and without NIR irradiation. A 75% decrease in cell viability was observed following NIR irradiation of nanorod-coated PA3 cells, along with a considerable increase in the number of dead or compromised cells (P<0.05). According to these findings, the functionalization of GNRs with antibodies and their exposure to NIR light determined the selective targeting of PA3 cells. Therefore, these complexes are able to reduce cell viability to a great extent.36
Figure 4 Transmission electron microscopy images (magnification: 30,000×) representing PA3 – antibody–nanorod conjugates interactions.
The characterization of high NIR absorbing multibranched gold nanocrosses (~35 nm) was presented by other authors.37 Antibody-functionalized gold nanocrosses were used for targeting PcrV, a protein involved in the translocation of type III secreted toxins of P. aeruginosa. The bacterial suspension of P. aeruginosa PAO1 was tested in the presence of the conjugates using confocal fluorescence microscopy to assess the effect produced by the photoexcitation of gold nanocrosses with strong NIR absorption. The experiment consisted of the following steps: the preparation of 500 μL PAO1 bacterial suspension (1×108 cells/mL), followed by incubation with antibody-functionalized gold nanocrosses for 1 hour (final concentration 0.1 mg/mL). Following the washing step, the effect produced by photoexcitation at 800 nm (power density of ≈ 3.0 W/cm) for 5 minutes was assessed using confocal microscopy. Total destruction of P. aeruginosa was accomplished by the nanocomplex following NIR irradiation therapy, with the absence of bacterial activity at 48 hours after treatment. This confirms the effective photothermal killing of bacteria and the prevention of bacterial regrowth. These gold nanocross conjugates proved to be successful for effectively targeted and localized photothermal ablation of MDR bacteria, reducing the risk or severity of damage to normal tissue.37
Nano-photothermal therapy mediated by GNPs in MDR Salmonella bacteria
In their study, Lin and Hamme developed an assay for the quick and accurate detection of bacteria by measuring the affinity binding of antibodies, the two-step, seed-mediated synthesis of popcorn-shaped GNPs (GNPop), the use of surface-enhanced Raman scattering, and the introduction of the inductively coupled plasma mass spectrometry analytical technique.38 A qualitative assessment indicated the presence of Salmonella in 10 minutes using Raman spectroscopy, while 100 isolates of Salmonella typhimurium definitive type 104 were detected in 40 minutes in 1 mL (100 CFU/mL) during a quantitative assessment. Concentration-dependent (CFU/mL) laser therapy (1 W/cm2, 670 nm) indicated the strong relationship between this type of treatment and the killing of Salmonella. Photothermal damage achieved by the conjugation of monoclonal M3038 antibody to GNPop resulted in the killing of nearly 100% of the bacteria (Salmonella, 105 CFU/mL) in 19 minutes. Another finding revealed an almost complete concentration-dependent (102–107 CFU/mL) bacterial destruction following laser irradiation for 6–30 minutes.38
Monoclonal antibody-conjugated popcorn-shaped gold nanotechnology was employed for selective killing of S. typhimurium DT104 resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline. Following exposure to 670 nm laser (200 mW/cm2), tryptic soy agar was used to assess complete killing of the bacteria.39
Nano-photothermal therapy mediated by GNPs in Enterococcus faecalis MDR bacteria
Another conjugate based on a catheter coating containing gold nanoshells (AuNSs) functionalized with carboxylate-terminated organosulfur compounds was developed40 to assess the antimicrobial effect against E. faecalis. The gold standards for nanoparticle characterization, transmission electron microscopy, and scanning electron microscopy, were used for imaging the morphology of AuNSs. Energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy were used to detect and map their components and analyze their surface chemistry. Also, ultraviolet–vis spectroscopy and infrared thermography helped quantify the optical and thermal properties of the conjugate. The time–exposure ratio that would cause killing of bacteria subjected to heat was assessed following treatment with an infrared (810 nm) diode laser using different exposure times. Conversely, the clonogenic assay was used to assess the survival of the bacteria after the inoculation of AuNPs conjugates, in either the presence or absence of NIR radiation. When compared to controls, killing of E. faecalis occurred to a greater extent after 5 minutes of treatment with NIR radiation, and this increased even more significantly after 10 minutes. In conclusion, efficient killing of E. faecalis adherent to silicone can be induced by irradiated AuNPs conjugates.
In another study,41 antibody-conjugated oval-shaped gold nanotechnology was employed for selective targeting and killing of pathogenic bacteria. Synthesis of nanoparticles using bacteria followed by NIR radiation induced the photothermal lysis of pathogenic bacteria, which subsequently reduced cell viability.
Nano-photothermal therapy mediated by GNPs in E. coli MDR bacteria
GNR-covered kanamycin-loaded hollow SiO2 (HSKAu rod) nanocapsules were developed for drug delivery and photothermal killing of bacteria, demonstrating that the association between a chemical drug, physical sterilization, and the physical damage of biological systems can make the sterilization more effective and deliver the expected results.42 SiO2 nanocapsules loaded with kanamycin and covered with GNRs were incubated with E. coli BL21 and exposed to NIR laser irradiation for 20 minutes. The treatment resulted in an overall rate of sterilization of 53.47%, and 34.49% for the individual components of the hybrid bactericidal material (carrier, chemical sterilization of kanamycin, and physical sterilization induced by NIR laser-excited GNRs).42 As they proved to accumulate on bacterial surfaces and possess two-photon-induced photoluminescence properties, positively charged gold nanospheres were used against bacteria by laser pulses with a duration recorded on the femtosecond time scale. Whether incubated or not with gold nanospheres, bacterial cells were subjected to femtosecond laser pulse irradiation with a power of 15 mW, at 820 nm, using the raster scan method and 8 ms dwell time. The Live/Dead cell viability assay was used to assess the killing effect triggered by these conjugates. SYTO 9 green fluorescent nucleic acid stain labeled all bacterial cells, while propidium iodide red fluorescent counterstain labeled compromised bacterial cells. When S. aureus or E. coli was not treated with gold nanospheres, no significant numbers of bacteria exhibited red fluorescence and were considered as dead cells, as the intact bacterial cell wall kept the red fluorescent stain from bursting in. The extent of bacterial killing was not considerable in these bacteria following irradiation. Treatment with gold nanospheres (7.5×10−12 M) and radiation therapy enhanced bacterial killing. One treatment was enough to cause 88.8% antibacterial effects against S. aureus and 82.6% against E. coli.
In another study,43 the development of a photon-to-thermal conversion nanosystem and exposure of GNRs to laser irradiation produced a quick increase in temperature in the media. Laser-excited GNRs were expected to cause photothermal lysis of the bacteria, which was examined by synthesizing GNRs (aspect ratio 3:4) and determining their optical and thermal properties. Short GNRs proved to absorb laser pulses (808 nm, 450 mW) to a greater extent than long GNRs, and temperature was significantly higher in glass vials. When induced by continuous wave laser irradiation, these nanorods were able to increase the temperature to above 100°C in bulk media; thus, E. coli cell lysis was obtained by exposure of the bacteria-containing culture to NIR laser irradiation.43
Huang et al reported the synthesis of polygonal GNPs with affinity for absorbing NIR light, using a photochemical reaction.24 Vancomycin, an antibiotic that binds the D-alanine (D-Ala) residues, was attached to the GNPs and the resulting construct was used for growth inhibition of pathogenic bacteria under NIR irradiation at 808 nm. The conjugate was also able to recognize gram-positive and gram-negative cell walls, as indicated by transmission electron microscopy (Figure 5). Nonetheless, the formation of bonds between unmodified GNPs and these bacteria was absent. GNPs were able to destroy >99% of bacteria (S. aureus, Streptococcus pyogenes, E. coli, MRSA) in 5 minutes under NIR irradiation as a result of the photothermal effect of this type of therapy.24
Figure 5 TEM images of (A) VRE, (B) PDRAB, and (C) VRSA produced after incubation with [email protected] in PBS. TEM images of (D) VRE, (E) PDRAB, and (F) MRSA produced after incubation with unmodified gold nanoparticles in PBS.
Although proof-of-principle experiments in which laser thermal ablation of MDR bacteria using bio-functionalized nanoparticles are reported in literature, yet, there is a need for further in vitro and in vivo, investigations. The potential for translation to human therapies is high, especially because GNPs exhibit low toxicity and chemical inertia.
This review shows that gold nanomaterials can be successfully used for photothermal treatment, paving the way for future materials with strong and selective biocide effects determined by combining colloid antibodies and strategies that induce cell killing, with possible applications in novel therapies against MDR bacteria. The new materials based on a chemical drug associated with physical sterilization trigger a synergistic effect and increase the effectiveness of sterilization, and are thus promising candidates for adjuvant antibacterial treatment and disease control.
The authors would like to thank Mr Cosmin Puia for his support in gathering data enounced in this manuscript.
This work was supported by the Romanian National Authority for Scientific Research and Innovation, CNCS-UEFISCDI, project numbers PN-II-RU-TE-2014-4-2267; PN-II-PTPCCA-2011-3.1-1586; PN-II-PT-PCCA-2011-3.1-1551; PN-II-PT-PCCA-2011-3.2-1289;PN-II-PT-PCCA- 2013-4-1553;PN-III- P2-2.1-BG-2016- 0446; PN-III-P2-2.1-PED-2016-1742; 4995/13/08.03.2016.
All authors contributed equally to this paper; made substantial contributions to conception and design, acquisition of data, or analysis and interpretation of data; took part in drafting the article or revising it critically for important intellectual content; gave final approval of the version to be published; and agree to be accountable for all aspects of the work.
The authors report no conflicts of interest in this work.
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