Back to Journals » Cancer Management and Research » Volume 11

Increased Expression Of SOX2 Predicts A Poor Prognosis And Promotes Malignant Phenotypes In Upper Tract Urothelial Carcinoma

Authors Bao Z, Zhan Y, He S, Li Y, Guan B, He Q, Yang X, Li X, Fang D, Zhou L

Received 15 June 2019

Accepted for publication 5 October 2019

Published 24 October 2019 Volume 2019:11 Pages 9095—9106


Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Dr Yong Teng

Zhengqing Bao,1–3,* Yonghao Zhan,1–4,* Shiming He,1–3 Yifan Li,1–3 Bao Guan,1–3 Qun He,1–3 Xinyu Yang,1–3 Xuesong Li,1–3 Dong Fang,1–3,5 Liqun Zhou1–3

1Department of Urology, Peking University First Hospital, Beijing, People’s Republic of China; 2Institute of Urology, Peking University, National Urological Cancer Centre, Beijing, People’s Republic of China; 3Beijing Key Laboratory of Urogenital Diseases (Male), Molecular Diagnosis and Treatment Center, Beijing, People’s Republic of China; 4Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450003, People’s Republic of China; 5Andrology Center, Peking University First Hospital, Beijing 100034, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Dong Fang; Liqun Zhou
Department of Urology, Peking University First Hospital, No. 8 Xishiku St, Xicheng District, Beijing 100034, People’s Republic of China
Tel +86-10-83575006
Fax +86-10-66551726

Background: The transcription factor SRY-related HMG-box 2 (SOX2) plays important regulatory roles in diverse biological processes (cell proliferation, migration, invasion and tumorigenicity). However, the relationship between SOX2 and upper tract urothelial carcinoma (UTUC) have not been intensively investigated. This study aims to analyze the expression of SOX2 in UTUC as well as the predictive value for prognosis and the effect on tumor aggressiveness of SOX2.
Methods: Formalin-fixed, paraffin-embedded blocks containing samples from 341 patients with UTUC who underwent radical nephroureterectomy (RNU) at our institute were analyzed for SOX2 expression by immunohistochemistry (IHC). Associations between the SOX2 expression level and clinicopathological characteristics, disease-free survival (DFS) and cancer-specific survival (CSS) were analyzed. SOX2 expression in a normal urothelial cell line, urothelial carcinoma cell lines, 16 UTUC tissues and their pair-matched adjacent normal tissues was evaluated by RT-qPCR. Using RNA interference in vitro, the effects of SOX2 inhibition on cell proliferation, migration, invasion and tumorigenicity were determined.
Results: SOX2 expression was significantly upregulated in UTUC tissue samples compared with paired-adjacent nontumorous tissue samples. SOX2 expression was correlated with important clinicopathological features, including tumor stage, tumor grade, tumor architecture and the presence of glandular or sarcoma differentiation, and was an independent predictor of poor DFS and CSS. Further experiments indicated that SOX2 expression was higher in UTUC cell lines than in a normal urothelial cell line. Knocking down SOX2 expression could inhibit malignant phenotypes (cell proliferation, stemness, migration, invasion and tumorigenicity) in UTUC cells.
Conclusion: SOX2 is an independent prognostic marker of poor DFS and CSS in UTUC patients who have undergone RNU. Moreover, these data suggest that SOX2 may be a promising therapeutic target in UTUC.

Keywords: SRY-related HMG-box 2, upper tract urothelial carcinoma, biomarker, prognosis, stemness

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]