Fabrication and physicochemical characterization of porous composite microgranules with selenium oxyanions and risedronate sodium for potential applications in bone tumors
Received 2 May 2017
Accepted for publication 2 June 2017
Published 7 August 2017 Volume 2017:12 Pages 5633—5642
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Dr Thomas J Webster
Joanna Kolmas,1 Kamil Pajor,1 Lukasz Pajchel,1 Agata Przekora,2 Grażyna Ginalska,2 Ewa Oledzka,3 Marcin Sobczak3
1Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy with Laboratory Medicine Division, Medical University of Warsaw, Warsaw, 2Department of Biochemistry and Biotechnology, Faculty of Pharmacy with Medical Analytics Division, Medical University of Lublin, Lublin, 3Department of Biomaterials Chemistry, Faculty of Pharmacy with Laboratory Medicine Division, Medical University of Warsaw, Warsaw, Poland
Abstract: Nanocrystalline hydroxyapatite containing selenite ions (SeHA; 9.6 wt.% of selenium) was synthesized using wet method and subject to careful physicochemical analysis by powder X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, solid-state nuclear magnetic resonance, wavelength dispersive X-ray fluorescence, and inductively coupled plasma optical emission spectrometry. SeHA was then used to develop the selenium-containing hydroxyapatite/alginate (SeHA/ALG) composite granules. Risedronate sodium (RIS) was introduced to the obtained spherical microgranules of a size of about 1.1–1.5 mm in 2 ways: during the granules’ preparation (RIS solution added to a suspension of ALG and SeHA), and as a result of SeHA/ALG granules soaking in aqueous RIS solution. The analysis made using 13C and 31P cross-polarization magic angle spinning nuclear magnetic resonance confirmed the presence of RIS and its interaction with calcium ions. Then, the release of selenium (inductively coupled plasma optical emission spectrometry) and RIS (high-performance liquid chromatography) from microgranules was examined. Moreover, cytotoxicity of fabricated granules was assessed by MTT test. Selenium release was biphasic: the first stage was short and ascribed to a “burst release” probably from a hydrated surface layer of SeHA crystals, while the next stage was significantly longer and ascribed to a sustained release of selenium from the crystals’ interior. The study showed that the method of obtaining microgranules containing RIS significantly affects its release profile. Performed cytotoxicity test revealed that fabricated granules had high antitumor activity against osteosarcoma cells. However, because of the “burst release” of selenium during the first 10 h, the granules significantly reduced viability of normal osteoblasts as well.
Keywords: composites, nanocrystalline hydroxyapatite, drug release, cytotoxicity, antitumor activity, ionic substitutions, biomaterials, bisphosphonates
Bone tissue defects are one of the most challenging problems in clinical orthopedics and surgical dentistry. Infections, trauma, bone tumors and congenital osseous diseases may all cause significant bone defects.1 Ranging from small lacks in flat bone to large voids in long bone, they all require specific techniques of reconstruction. In many cases, the “gold standard” in terms of osteointegration is obtained using autologous bone graft.2 However, a limited supply of this type of bone graft and the risk of chronic pain or infection at the explant site reduce its use. Allografts and xenografts may eliminate these limitations, but problems with immune reactions, rejections and slower osteointegration can occur.3,4
Nowadays, attention has been focused on the use of synthetic biomaterials holding the ability to replace defective bone tissue.5 Synthetic bone substitutes based on calcium phosphate materials (CaPs) in various forms (ie, granules, cements, pastes, geometric blocks) are commonly used in bone reconstruction. Among CaPs used as biomaterials, hydroxyapatite (HA; Ca10(PO4)6(OH)2) is the most popular in biomaterials engineering, due to its similarity to the mineral component of nature bone and dental mineralized tissues.6,7 Moreover, it was reported that HA could be substituted by various ions that have an impact on bone metabolism, bone formation or bone disease therapy.8 Unfortunately, low mechanical strength significantly limits the use of HA. Therefore, recently HA materials have been used as polymer/ceramic composite components, which can not only fulfill osseous tissue defects but also deliver drugs directly to the bone tissue.9,10 Polymeric components improve mechanical properties and allow the controlled release of a drug.
The aim of the present work was the preparation and physicochemical and biological characterization of microgranules containing selenium-substituted nanocrystalline HA, alginate sodium and risedronate sodium (RIS).
Selenium is a trace element of human tissues, playing an important role as a component of antioxidant enzymes and selenoproteins.11 Its deficiency may produce serious diseases, that is, Keshan disease (cardiomyopathy in children) or severe chondrodystrophy.12 It has been reported that selenium has a great impact on bone density and metabolism.13 Additionally, numerous studies have declared its chemopreventive and therapeutic activity against various types of cancers.14–17
ALG is a natural ionic polymer with L-guluronate and D-mannuronate as major components. It is typically extracted from brown algae (Phaeophyceae).18,19 This polysaccharide is biodegradable and biocompatible and due to cross-linking properties, able to form gels, beads, fibers and foams. ALGs have been combined with various bioceramics to form promising scaffolds for tissue regeneration and drug-delivery systems.20,21
RIS belongs to the bisphosphonates (BPs) group. BPs are commonly used as antiresorptive agents for the treatment of osteoporosis, Paget’s disease or bone tumors and bone metastasis.22,23 RIS has potency to inhibit osteoclast-mediated bone resorption. Moreover, RIS acts as an inhibiting factor for tumor cells’ adhesion and proliferation. However, doses of RIS for their antitumor effect should be high. It should be also noted that the absorption of RIS after standard, oral administration and its bioavailability are very low, thus problems with significant side effects and insufficient efficacy may occur.24,25
In our work, we synthesized nanocrystalline HA enriched in selenium-containing hydroxyapatite (SeHA) and we prepared and characterized microgranules composed of ALG and SeHA (selenium-containing hydroxyapatite/alginate [SeHA/ALG]). In the next step, we produced SeHA/ALG microgranules containing RIS by 2 different routes. Together with their chemopreventive and anticancer activity, these composites were designed to support the bone regeneration. The physicochemical properties of the obtained SeHA powder and microgranules were investigated in detail by several analytical methods: powder X-ray diffractometry (PXRD), Fourier transform infrared spectroscopy (FT-IR), solid-state nuclear magnetic resonance (ssNMR), transmission and scanning electron microscopy (TEM and SEM) and wavelength dispersive X-ray fluorescence (WD-XRF). Moreover, the release profiles of selenium and RIS from the composite granules were examined by inductively coupled plasma optical emission spectrometry (ICP-OES) and high-performance liquid chromatography (HPLC), respectively. To evaluate potential antitumor activity of produced granules, preliminary biological test using osteosarcoma-derived cell line was performed in in vitro conditions.
Materials and methods
All the reagents for the synthesis of SeHA, alginate sodium and diammonium carbonate ([NH4]2CO3) were purchased from Sigma-Aldrich Inc. (St Louis, MO, USA). RIS of USP Reference Standard grade was obtained from USP (Rockville, MD, USA). Calcium chloride, potassium chloride and acetonitrile of HPLC grade were purchased from Avantor Performance Materials (Gliwice, Poland). Tetrabutylammonium hydroxide solution 40.0% (v/v) in water, sodium pyrophosphate and glacial acetic acid, reagents all of analytical grade, were purchased from Sigma-Aldrich Inc. Water was deionized and further purified by means of a Milli-Q Water Purification System (Millipore, Molsheim, France). The standard solutions of selenium 1,000 μg/mL were purchased from J.T. Baker (Deventer, Holland).
Materials for cell culture experiment
Human osteoblast-like cell line derived from osteosarcoma (Saos-2), normal human fetal osteoblasts (hFOB 1.19) and McCoy’s 5A culture medium were purchased from ATCC-LGC Standards (Teddington, UK). Fetal bovine serum (FBS) was obtained from Pan-Biotech (Aidenbach, Germany). 25 cm2 T-flasks, 96-multiwell plates, PBS, DMEM/Ham F12 medium, G418, penicillin-streptomycin solution, MTT, and dodecyl sulfate sodium salt (SDS) were purchased from Sigma-Aldrich Chemicals (Lublin, Poland). Hydrochloric acid was supplied by Avantor Performance Materials.
Preparation of SeHA
For the preparation of SeHA for this study, we used the wet method previously described.26 Briefly, 50 mL of 0.5 M (NH4)2HPO4 solution was added to 50 mL of 0.08 M Na2SeO3·5H2O and dropwise added to 400 mL of 0.1 M Ca(NO3)2·4H2O solution. Throughout the reaction, the pH was stabilized at 9.5 using ammonium hydroxide solution. The temperature was controlled at 70°C. The mixture was allowed to age for further 24 h at room temperature. The suspension was then filtered and washed several times with distilled water and dried overnight at 130°C. For comparison, pure unsubstituted HA was synthesized via the same route.
SeHA and HA powders were used to prepare porous apatite/alginate granules (SeHA/ALG) and (HA/ALG), respectively. First, a viscous 5% aqueous solution of ALG was obtained by intensive stirring at 40°C. Then 2 g of fine powder of SeHA (HA) and 0.5 g of NH4HCO3 were added to 20 mL of ALG solution. The obtained slurry was mixed carefully and then squeezed by a syringe needle into the 1.5% CaCl2 solution. The small spherical drops were produced due to the cross-linking between Ca2+ cations and the carboxylate anions of glucuronate units in ALG. The obtained granules were washed several times with water and dried at 40°C for 48 h.
Preparation of RIS-loaded granules (SeHA/ALG/RIS)
First route (SeHA/ALG/RIS I)
During the preparation of porous granules, RIS was added (50 mg) to 5% ALG solution. Then the SeHA powder and NH4HCO3 were added to obtain dense slurry (identical to that in the granules’ preparation). The slurry was used to produce the small porous granules that were washed with distilled water and dried at 40°C for 48 h.
Second route (SeHA/ALG/RIS II)
RIS was dissolved in 1 mmol/L KCl solution at a concentration of 0.01 mmol per 1 mL. Then the granules were soaked for 2 h in the above RIS solution in amounts of 1 granule per 1 mL. The granules were washed gently with KCl solution and dried at 40°C for 48 h.
Release of selenium and RIS sodium from the microgranules
For dissolution of selenium from selenium-containing granules, 3 g of granules were added to a round-bottomed flask, using 100 mL of buffer pH =7.4 at 37°C±0.5°C as the dissolution medium. Samples aliquots of 10 mL were taken at 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, 144, 168 and 192 h and filtered through 0.22 μm nylon filters into the glass flask. Every time a sample was taken, 10 mL of buffer was added to the round-bottomed flask.
For the dissolution of RIS from RIS-loaded granules, 15 mg of granules were added to the round-bottomed flask, using 5 mL of buffer pH =7.4 at 37°C±0.5°C as the dissolution medium. Samples aliquots of 1 mL were taken at 1, 5, 10, 20, 30, 45, 60, 90, 120, 150, 180, 210, 240 and 300 min and filtered through 0.22 μm nylon filters into the glass flask. Every time a sample was taken, 1 mL of buffer was added to the round-bottomed flask.
PXRD analysis of HA powders was performed using a Bruker D8 Discover diffractometer (a step size 0.024° and a 2Θ range from 20 to 70°; Bruker, Poznań, Poland). For estimation of crystallite size, we calculated the values of full width at half maximum of the reflection of the (002) and (300) planes, representing the crystallites along the c-axis and a-axis, respectively. The Scherrer formula was used.27
The crystals’ morphology was determined using High Performance Transmission electron microscope JEM 1400 (JEOL Co., Tokyo, Japan) equipped with 11 Megapixel TEM Camera MORADA G2 (EMSIS GmbH, Münster, Germany).
The chemical composition of apatitic powders (Ca, P and Selenium content) was determined by using WD-XRF (Advant’XP; Thermo-ARL; ARL, Lausanne, Switzerland).
FT-IR measurements were carried out using a Spectrum 1000 spectrometer working at mid-infrared range (Spectrum 1000; Perkin Elmer, Warsaw, Poland). The obtained samples (apatitic powders and composite granules) were mixed with KBr in weight ratio 1:100 and pressed into pellets.
The magic angle spinning nuclear magnetic resonance (MAS NMR) spectra of 31P and 13C nuclei were recorded using a Bruker Avance 400 WB spectrometer. For 31P and 13C MAS NMR experiments, the samples were spun at 7 and 7.5 kHz, respectively. In all the experiments, the cross-polarization technique was used. 31P spectra were taken at a resonance frequency at 160 MHz with a10 s delay time, 2 ms contact time and a π/2 pulse of 2.7 μs. The 13C CP MAS NMR spectra were recorded with a 15 s delay time, 2 ms contact time and a π/2 pulse of 2.4 μs. The chemical shifts were referenced to tetramethylsilane and 85% H3PO4, for 13C and 31P NMR, respectively.
RIS in degradation solutions was determined by HPLC using the chromatographic equipment consisting of a Varian Prostar 210 isocratic pump (Palo Alto, CA, USA) and a Rheodyne 7725i injector (Cotati, CA, USA) with a 50 mL sample loop. Detection was performed by a Varian Prostar 325 ultraviolet detector, using a detection wavelength of 262 nm.
Chromatographic conditions and the measurement procedure were previously described.28 The LC column used was a 4.6 mm i.d.×250 mm length XTerra RP 18 analytical column that was purchased from Whaters (Milford, Ireland). The mobile phase consisted of 0.01 M tetrabutylammonium hydroxide and 0.005 M sodium pyrophosphate (pH 7.0) mixed with acetonitrile in a ratio (78:22, v/v). It was degassed by sonication prior to use.
The flow rate of the mobile phase was maintained at 1 mL/min. HPLC analysis was conducted at ambient temperature. Peak areas were measured for the quantitation of the RIS. Stock standard solutions of RIS 100.00 μg/mL were prepared by dissolving the appropriate amount in water. Calibration standards were prepared over the concentration range of 0.50, 1.00, 10.00, 20.00, 30.00, 40.00, 60.00 and 80.00 μg/mL for RIS by appropriate dilutions of the above-mentioned stock standard solution in 10.0 mL of buffer. Calibration standards were analyzed in triplicate for the calibration curve.
Selenium in degradation solutions was determined by ICP-OES using an Optima 3100XL spectrometer (Perkin Elmer).
Selenium calibration standards were prepared over the concentration range of 1.00, 10.00, 20.00, 50.00, 150.00 and 300.00 μg/mL for selenium by appropriate dilutions of the stock standard solutions of selenium 1,000 μg/mL in 10.0 mL of buffer. Calibration standards were analyzed in triplicate for the calibration curve.
Cell culture experiment
Human tumor cells derived from osteosarcoma (Saos-2) were cultured in McCoy’s 5A medium supplemented with 15% FBS and antibiotics: 100 U/mL penicillin, 100 μg/mL streptomycin. hFOB 1.19 were cultured in a 1:1 mixture of DMEM/Ham F12 medium without phenol red supplemented with 10% FBS and antibiotics: 300 μg/mL G418, 100 U/mL penicillin, 100 μg/mL streptomycin. Saos-2 cells were maintained at 37°C and hFOB 1.19 cells at 34°C (ATCC recommendations) in a humidified atmosphere of 5% CO2 and 95% air. Cytotoxicity of prepared granules against tumor and normal osteoblasts was assessed indirectly using extracts of the granules. Both, extraction procedure and cytotoxicity test were performed in accordance with international standards ISO 10993–5.29 Extracts were prepared via incubation of granules in a complete culture medium for 24 h at 37°C. Before the extraction, highly absorbent granules were completely soaked in the medium, then 1 mL of culture medium was added per every 100 mg of granules (weight in a dry state was considered). Upon 24 h extraction, the samples were centrifuged and supernatants (extracts) were collected. Culture media incubated without the granules for 24 h at 37°C served as a negative control of cytotoxicity (100% viability). 100% and 50% (diluted using medium) extracts of the granules were tested.
The cells were seeded in 96-multiwell plates in 100 μL of appropriate culture medium at a concentration of 3×105 Saos-2 cells/mL (3×104 cells per well) and 1.5×105 hFOB 1.19 cells/mL (1.5×104 cells per well). Upon 24 h incubation at 37°C, when tumor and normal cells reached 90% confluence, the culture media were replaced with 100 μL of granule extracts. After 24 h exposure to the extracts, viability of cells was determined using MTT test. Briefly, 25 μL of 5 mg/mL MTT solution in PBS was added to each well, plates were incubated for 3 h at 37°C, and then formazan crystals formed in viable cells were dissolved using 10% SDS solution prepared in 0.01M HCl. Absorbance (Abs) of resultant solution was measured at 570 nm using Bio Tek Synergy H4 Hybrid Microplate Reader (Swindon, UK). The test was carried out in 3 independent experiments (n=3) and performed in quadruplicate. The cell viability was determined based on the obtained Abs values using the following formula:
Results, obtained with MTT test, were analyzed using GraphPad Prism 5, Version 5.03 Software (GraphPad Software, Inc., La Jolla, CA, USA). The unpaired t-test was applied to assess whether reduction in cell viability compared with the negative control (100% viability) was statistically significant (P<0.05).
Results and discussion
During the synthesis, the amount of reagents was calculated by assuming the mechanism of substitution proposed in our previous work.26
To maintain a charge balance, substitution of trivalent phosphate anion with divalent selenite anion should be compensated by simultaneous release of calcium cation and hydroxyl anion. In this work, we have chosen a high concentration of selenium (x=1.2; 9.70 wt.%). The selenium content, determined by WD-XRF, was slightly lower than assumed (9.60 wt.%).
PXRD patterns of dried “pure” HA and selenium-enriched (SeHA) powders are shown in Figure S1. Both diffractograms show only the HA reflections (JCPDS #09–0432). However, the SeHA pattern has broader reflections than “pure HA”, which means fine crystallites and lower crystallinity.26,30 The average crystallite sizes (in nm), as determined using Scherrer’s equation along the a and c crystallographic axes, with unit cell parameters is presented in Table S1. The crystallite size of the SeHA sample is significantly smaller than “pure HA” and amounts to <10 nm.
Particle morphology of the obtained powders was investigated by TEM. The representative TEM image of the SeHA sample is shown in Figure 1. The SeHA material is composed of nano-sized, plate-like, slightly elongated particles with a great tendency to agglomerate.
Figure 1 TEM image of the SeHA powder.
FT-IR and 31P ssNMR spectroscopies were used to analyze the effect of the selenite substitution on the structure (Figures S2 and S3). The FT-IR spectrum of SeHA confirms a poorly crystalline (nanocrystalline) apatitic structure. The main phosphate bands (1,200–1,900 cm−1) are broader and poorly resolved.31
A very weak band at about 3,570 cm−1 can be observed in the spectrum of the SeHA sample, which corresponds to the vibrations of structural OH groups. The libration bands of OH groups (at about 630 cm−1) are not visible. Relative intensity of the bands derived from water (at about 3,440 cm−1 stretching vibrations, and 1,633 cm−1 bending vibrations) is much larger for the SeHA sample. It is worth remembering that the crystals in SeHA material are very fine and have a strong tendency to form clusters.32 Water from the interior of these clusters cannot be removed completely during drying at a moderate temperature (~130°C).
The bands 1,450–1,418 cm−1 on the spectrum are evidence of trace amounts of carbonate ions, which could be introduced into the structure as impurities. Their number was estimated using the Clasen and Ruyter method as <0.5 wt.%.33 These were easily detected at 767 cm−1 and were much less intense at 730 cm−1, corresponding to the vibrations of SeO32− groups. It can be noticed concurrently that there are no bands derived from other selenium ions on the spectrum.23
31P NMR spectra are shown in Figure S3 (in the Supplementary materials). The presence of selenite (IV) ions in SeHA material results in a significant widening of the 31P NMR line at about 3.1 ppm, corresponding to phosphorus-31 nuclei in SeHA. This proves a decrease in material crystallinity and at the same time, the development of a hydrated surface layer.34 Our recent 77Selenium NMR studies showed that SeO32− ions in HA are localized not only within the crystal lattice in the position of PO43− ions, but also in the hydrated surface layer. We can assume that the location of these ions will determine their release from the material.
The next step was to prepare porous microgranules containing SeHA. For this purpose, we used ALG, which was able to cross-link as a result of the exchange of sodium ions for calcium ions. Figure 2 shows the images of microgranules obtained by the SEM method. The collected microgranules have a spherical shape and their size varies in the range of 1.1–1.5 mm in diameter. Figure 2A presents a number of larger pores, which were formed by the addition of ammonium carbonate. Enlarged image (cross-section) B reveals both the presence of large pores and the smaller ones (~10 μm) related to the porous structure of HA-ALG composite.
Sorption studies demonstrated that the granules’ material is microporous. The specific surface area SBET was about 83±5 m2/g and total pore volume was 0.21 cm3/g, including mesopores of 0.15 cm3/g. The pores with diameter of 20–60 nm dominated in the material.
SeHA/ALG microgranules with risedronate sodium
Two kinds of granules containing RIS were prepared according to the procedure described in the Materials and methods section. The resulting materials were tested using NMR spectroscopy. Figure 3 presents 31P CP NMR spectra for both samples: with RIS introduced into the granules during their formation (SeHA/ALG/RIS I) and soaked in a solution of risedronate (SeHA/ALG/RIS II). In addition, the spectra of “pure” granules, which do not contain risedronate and pure RIS, were made for comparison (Figure 3). The signal at 3.1 ppm characteristic of the nuclei of phosphorus-31 HA dominates in spectra of both types of granules (SeHA; Figure S3).35 A quite broad and low-intensity signal at about 15.9 ppm is visible in the spectrum SeHAAlg/RIS I, while in the spectrum SeHA/ALG/RIS II, it is considerably broader and less intense. We can assume that these signals come from the nuclei of RIS phosphorus-31.36,37 The spectrum of RIS in this area shows two intense, slightly separated lines at about 17.4 and 19.4 ppm corresponding to the hemipentahydrated form of RIS. Two resonance lines correspond to the 2 non-identical phosphonate groups. The presence of the shifted signal in the spectra of both types of granules indicates a change in the chemical surrounding of phosphonate groups as a result of interaction with calcium ions Ca2+.36
Figure 3 31P CP MAS NMR of the obtained materials. (νMAS =7 kHz).
13C NMR spectra of the examined granules are presented in Figure 4. Also, the spectra of pure RIS and ALG, from which the granules were obtained, are presented for comparison. Dominant signals from ALG (designated as A) are present in the spectra of the granules. Additionally, low-intensity signals in the area of 100–120 ppm and below 50 ppm can be noticed, which can be attributed to RIS present in the samples (lines marked as R).36,38 By analyzing the mutual intensity of R/A lines in both samples, it can be easily noticed that the RIS share in granules obtained using the first method is higher than in granules obtained using the second method. Slight shifts of signals R, as in the spectra 31P NMR, demonstrate the interaction of RIS with calcium ions.
Selenium and risedronate release
Figure 5 shows the release of selenium from composite granules SeHA/ALG to PBS medium with a pH value of 7.4. It is easy to notice a high “burst release” rate in the first 10 h of the experiment. After this time, the process of constant release increase up to 200 h can be observed. During the first stage, there is probably the release of selenite ions located in the hydrated surface layer of the crystals.32,34 This is followed by gradual dissolution of HA crystals and SeO32− ion release from the inside of crystals. The dissolution of HA enriched with selenium, although pH =7.4, is significant. This is primarily due to the morphology and size of SeHA crystals – crystals are very small and characterized by a strongly developed surface area.
Figure 5 Selenium release profile from the SeHA/ALG microgranules.
RIS release from both types of granules is shown in Figure 6. In the case of granules soaked in RIS solution, the release curve is characterized by slight “burst release” within about 10 min. This is probably related to the release of risedronate adsorbed just at the surface of the granules. Then, the release occurs at a lower rate, which corresponds to the transition of RIS adsorbed within the porous structure of microgranules to the PBS solution.
Figure 6 Risedronate release kinetics.
The release curve of RIS for the SeHA/ALG/RIS I sample has a completely different course. It can be said that the relationship of the amount of RIS released over time is linear, and therefore RIS passes into solution at a constant rate. We can therefore say that the way to prepare granules loaded with RIS significantly affects the profile of its release to the PBS solution at pH =7.4. The introduction of the drug during the preparation of SeHA/ALG/RIS I granules results in its better distribution in microgranules. It should also be noted that much more RIS was released in the process of soaking in RIS solution. However, it is not possible to draw reliable conclusions at this stage. It should only be assumed that the reason may be its higher content in SeHA/ALG/RIS I granules. The aim of this part of the study was only comparison of RIS release profiles from granules prepared in different ways.
Cytotoxicity against osteosarcoma cells and normal osteoblasts
MTT cytotoxicity assay revealed that upon 24 h incubation, composite granules composed of SeHA (SeHA/ALG) and also materials additionally containing RIS (SeHA/ALG/RIS I and II), significantly (P<0.0001) reduced viability of both Saos-2 and hFOB 1.19 cells by over 90% compared with the corresponding control cells (Figure 7). No significant differences between cytotoxicity of SeHA/ALG and SeHA/ALG RIS (I and II) extracts were observed, indicating that amount of released selenium from HA was sufficient to provide high antitumor activity of the granules. As a consequence, activity of RIS was masked by high cytotoxic effect of selenium ions. Extract of the control HA/ALG granules showed slight, but statistically significant (P=0.0076 for 100% extract, P=0.0295 for 50% extract in the case of Saos-2 cells and P=0.0001 for 100% extract in the case of hFOB 1.19 cells), reduction in cell viability to 78%–92% compared with the negative control of cytotoxicity. However, according to ISO 10993–5, 100% extract of the implantable material causing reduction in cell viability by 30% or less should be considered as non-cytotoxic. Thus, extract of control HA/ALG granules was demonstrated to be non-cytotoxic, proving that high cytotoxic effect against the cells was a result of selenium and RIS release from the composite granules. Moreover, according to mentioned standards, the results of the test are considered to be trustworthy if cells exposed to 50% extracts maintain the same or higher viability as cells exposed to 100% extracts.29 Since viability of Saos-2 and hFOB 1.19 cells exposed to 50% extracts of granules was slightly higher than viability of the cells exposed to 100% extracts, it may be concluded that obtained results are reliable.
In this study, extracts of the granules were prepared in accordance with ISO 10993–5 by 24 h incubation of the materials in culture medium without preincubation in aqueous solution and without any medium exchange. Thus, high cytotoxic effect of all microgranules against osteosarcoma and normal cells was probably the result of “burst release” of selenium ions during 24 h extraction time. Accumulated in the extracts, high concentrations of selenium ions caused effective tumor cells’ death but it also affected viability of normal osteoblasts. Nevertheless, fabricated in this study, microgranules were designed to be implanted in bone defects just after tumor resection. Taking into account potential biomedical application of produced composite granules, critically high concentration of selenium during the first 24 h upon implantation would be desired phenomenon since it would provide high antitumor protection. It should be noted that after implantation of the granules in living human body – where physiological body fluids constantly flow – just after “burst release” occurring during the first 10 h, the concentration of selenium in the surrounding microenvironment would be probably significantly reduced (due to body fluids flow and Se-release profile occurring at a lower rate, Figure 5). As a consequence, lower and less cytotoxic against normal cells concentration of selenium would be observed at the site of implantation. However, to fully confirm this hypothesis, it is planned to conduct cell culture experiments with the use of extracts prepared applying 2-step procedure, which is not in accordance with ISO 10993–5: 1) preincubation of the materials in culture medium for 24 h, 2) medium renewal followed by 24 h incubation in culture medium to obtain extracts.
This paper describes the synthesis of SeHA material enriched in selenium ions and a method for preparing the new SeHA/ALG composite containing selenite(IV) ions and RIS. According to physicochemical analysis, the obtained SeHA is nanocrystalline and is characterized by a high specific surface. The content of selenium in the form of selenite(IV) ions in HA was determined at 9.60% by weight. SeHA material was used to obtain porous microgranules, which were then loaded with RIS in 2 ways. The study analyzed the release of both selenium and the drug. In the first step, selenium is released from the hydrated surface layer of SeHA crystals, and then progressively from the interior of the crystals, which is related to their dissolution in PBS. The release of RIS is dependent on the method of the microgranules’ preparation. Moreover, it was demonstrated that fabricated granules release selenium and RIS in amounts sufficient to provide high protection against tumor development at the site of implantation in oncology patients. However, during the first 10 h, when “burst release” of selenium ions is observed, composite microgranules would also reduce viability of normal osteoblasts. Nevertheless, fabricated granules appear to be a very promising implantable material to be used as bone fillers just after tumor resection in patients susceptible to bone tumor relapse.
This work was supported by the research grant (Project NCN DEC-2011/03/D/ST5/05793) of the National Science Center, Poland. Financial assistance was partially provided by the Ministry of Science and Higher Education in Poland within the DS2 project of the Medical University of Lublin. The paper was partially developed using the equipment purchased within the agreement No POPW.01.03.00-06-010/09-00 Operational Programme Development of Eastern Poland 2007–2013, Priority Axis I, Modern Economy, Operations 1.3. Innovations Promotion.
The authors report no conflicts of interest in this work.
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Figure S1 PXRD patterns of the SeHA and HA samples.
Figure S2 FT-IR spectra of the SeHA and HA samples.
Figure S3 31P CP MAS NMR spectra of the SeHA and HA samples (νMAS =7.0 kHz).
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