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Ethosomal Gel for Improving Transdermal Delivery of Thymosin β-4

Authors Fu X, Shi Y, Wang H, Zhao X, Sun Q, Huang Y, Qi T, Lin G

Received 27 August 2019

Accepted for publication 16 November 2019

Published 27 November 2019 Volume 2019:14 Pages 9275—9284

DOI https://doi.org/10.2147/IJN.S228863

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Phong A Tran


Xianglei Fu,1 Yanbin Shi,2 Hui Wang,1 Xiaogang Zhao,3 Qifeng Sun,3 Yi Huang,1 Tongtong Qi,1 Guimei Lin1

1School of Pharmaceutical Science, Shandong University, Jinan 250012,People’s Republic of China; 2School of Mechanical & Automotive Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, People’s Republic of China; 3The Second Hospital of Shandong University, Jinan 250033, People’s Republic of China

Correspondence: Guimei Lin
School of Pharmaceutical Science, Shandong University, 44 West Wenhua Road, Jinan 250012, People’s Republic of China
Tel +86-531- 88382548
Fax +86-531-89631517
Email guimeilin@sdu.edu.cn

Purpose: Thymosin β-4(Tβ-4) is a macromolecular protein drug with potential for drug development in wound repair but is limited by the shortcomings of macromolecular protein, such as large volumes, poor membrane permeability, and unstable physicochemical characteristics. Ethosomes could enhance cell membrane fluidity and reduce epidermal membrane density to make macromolecular drugs through the stratum corneum into the deeper layers of the skin easily. Herein, we developed and characterized a novel transdermal delivery vehicle to load macromolecular protein peptides and use Tβ-4 as a model drug wrapped into ethosomes.
Methods: We used the orthogonal method to optimize the formulation of the ethosome preparation prepared by the ethonal infusion method. Ethosomal gels were characterized by using different analytical methods. Transdermal release rate in vitro have been demonstrated in Franz diffusion cells and the efficacy of drug-loaded nanocarriers in vivo was investigated in a mouse model.
Results: Optimized Tβ-4 ethosomal gels have good physicochemical properties. The drug amounts of the cumulative release in the ethosomal gel within 5 hours were 1.67 times that of the T-β4 gel in vitro release study, and the wound healing time of ethosomal gel group was only half of the T-β4 gel group in vivo pharmacokinetic study. Compared with the free drug group, the ethosome preparation not only promotes the percutaneous absorption process of the macromolecular protein drugs but also shortened wound recovery time.
Conclusion: Hence, we provide a possible good design for ethosomal gel system that can load macromolecular protein peptide drugs to achieve transdermal drug administration, promoting the percutaneous absorption of the drug and improving the effect.

Keywords: ethosomes, transdermal drug delivery system, macromolecular protein drugs, skin wound healing

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