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Dual delivery of active antibactericidal agents and bone morphogenetic protein at sustainable high concentrations using biodegradable sheath-core-structured drug-eluting nanofibers

Authors Hsu YH, Lin C, Yu Y, Chou Y, Liu S, Chan E

Received 26 February 2016

Accepted for publication 23 May 2016

Published 17 August 2016 Volume 2016:11 Pages 3927—3937

DOI https://doi.org/10.2147/IJN.S107250

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Lei Yang


Yung-Hen Hsu,1,2 Chang-Tun Lin,2 Yi-Hsun Yu,1,2 Ying-Chao Chou,1,2 Shih-Jung Liu,1,2 Err-Cheng Chan3

1Department of Orthopedic Surgery, Chang Gung Memorial Hospital, 2Department of Mechanical Engineering, 3Department of Medical Biotechnology and Laboratory Science, School of Medical Technology, Chang Gung University, Tao-Yuan, Taiwan


Abstract: In this study, we developed biodegradable sheath-core-structured drug-eluting nanofibers for sustainable delivery of antibiotics (vancomycin and ceftazidime) and recombinant human bone morphogenetic protein (rhBMP-2) via electrospinning. To prepare the biodegradable sheath-core nanofibers, we first prepared solutions of poly(D,L)-lactide-co-glycolide, vancomycin, and ceftazidime in 1,1,1,3,3,3-hexafluoro-2-propanol and rhBMP-2 in phosphate-buffered solution. The poly(D,L)-lactide-co-glycolide/antibiotics and rhBMP-2 solutions were then fed into two different capillary tubes controlled by two independent pumps for coaxial electrospinning. The electrospun nanofiber morphology was observed under a scanning electron microscope. We further characterized the in vitro antibiotic release from the nanofibers via high-performance liquid chromatography and that of rhBMP-2 via enzyme-linked immunosorbent assay and alkaline phosphatase activity. We showed that the biodegradable coaxially electrospun nanofibers could release high vancomycin/ceftazidime concentrations (well above the minimum inhibition concentration [MIC]90) and rhBMP-2 for >4 weeks. These experimental results demonstrate that novel biodegradable nanofibers can be constructed with various pharmaceuticals and proteins for long-term drug deliveries.

Keywords: biodegradable sheath-core nanofibers, coaxial electrospinning, vancomycin, ceftazidime, rhBMP-2, release characteristics

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