Back to Journals » International Journal of Nanomedicine » Volume 7

Controlled-release and preserved bioactivity of proteins from (self-assembled) core-shell double-walled microspheres

Authors Yuan W, Liu Z

Received 25 October 2011

Accepted for publication 13 November 2011

Published 13 January 2012 Volume 2012:7 Pages 257—270

DOI https://doi.org/10.2147/IJN.S27621

Review by Single-blind

Peer reviewer comments 2

Weien Yuan1,2, Zhenguo Liu1
1Department of Neurology, Xinhua Hospital, affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 2School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People’s Republic of China

Abstract: In order to address preserved protein bioactivities and protein sustained-release problems, a method for preparing double-walled microspheres with a core (protein-loaded nanoparticles with a polymer-suspended granule system-formed core) and a second shell (a polymer-formed shell) for controlled drug release and preserved protein bioactivities has been developed using (solid-in-oil phase-in-hydrophilic oil-in-water (S/O/Oh/W)) phases. The method, based on our previous microsphere preparation method (solid-in-oil phase-in-hydrophilic oil-in-water (S/O/Oh/W), employs different concentric poly(D,L-lactide-co-glycolide), poly(D,L-lactide), and protein-loaded nanoparticles to produce a suspended liquid which then self-assembles to form shell-core microspheres in the hydrophilic oil phase, which are then solidified in the water phase. Variations in the preparation parameters allowed complete encapsulation by the shell phase, including the efficient formation of a poly(D,L-lactide) shell encapsulating a protein-loaded nanoparticle-based poly(D,L-lactide-co-glycolide) core. This method produces core-shell double-walled microspheres that show controlled protein release and preserved protein bioactivities for 60 days. Based upon these results, we concluded that the core-shell double-walled microspheres might be applied for tissue engineering and therapy for chronic diseases, etc.

Keywords: protein delivery, protein stability, core-shell microspheres, dextran nanoparticles

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Estrogen-mediated hemangioma-derived stem cells through estrogen receptor-α for infantile hemangioma

Zhang L, Wu H, Yuan W, Zheng J

Cancer Management and Research 2017, 9:279-286

Published Date: 7 July 2017

Biodegradable and biocompatible cationic polymer delivering microRNA-221/222 promotes nerve regeneration after sciatic nerve crush

Song J, Li X, Li Y, Che J, Li X, Zhao X, Chen Y, Zheng X, Yuan W

International Journal of Nanomedicine 2017, 12:4195-4208

Published Date: 2 June 2017

Propranolol therapy for infantile hemangioma: our experience

Zhang L, Wu HW, Yuan W, Zheng J

Drug Design, Development and Therapy 2017, 11:1401-1408

Published Date: 8 May 2017

Advances with microRNAs in Parkinson’s disease research

Ma L, Wei L, Wu F, Hu Z, Liu Z, Yuan W

Drug Design, Development and Therapy 2013, 7:1103-1113

Published Date: 1 October 2013

Long-acting preparations of exenatide

Cai Y, Wei L, Ma L, Huang X, Tao A, Liu Z, Yuan W

Drug Design, Development and Therapy 2013, 7:963-970

Published Date: 5 September 2013

pH-sensitive degradable nanoparticles for highly efficient intracellular delivery of exogenous protein

Xu D, Wu F, Chen Y, Wei L, Yuan W

International Journal of Nanomedicine 2013, 8:3405-3414

Published Date: 2 September 2013

Hemostatic absorbable gelatin sponge loaded with 5-fluorouracil for treatment of tumors

Sun W, Chen Y, Yuan W

International Journal of Nanomedicine 2013, 8:1499-1506

Published Date: 18 April 2013

Advances with RNA interference in Alzheimer’s disease research

Chen S, Ge X, Chen Y, Lv N, Liu Z, Yuan W

Drug Design, Development and Therapy 2013, 7:117-125

Published Date: 22 February 2013

Sustained-release G-CSF microspheres using a novel solid-in-oil-in-oil-in-water emulsion method

Liu G, Hong X, Jiang M, Yuan W

International Journal of Nanomedicine 2012, 7:4559-4569

Published Date: 17 August 2012

Controlled-release levodopa methyl ester/benserazide-loaded nanoparticles ameliorate levodopa-induced dyskinesia in rats

Yang X, Zheng R, Cai Y, Liao M, Yuan W, Liu Z

International Journal of Nanomedicine 2012, 7:2077-2086

Published Date: 19 April 2012

A scalable fabrication process of polymer microneedles

Yang S, Feng Y, Zhang L, Chen N, Yuan W, Jin T

International Journal of Nanomedicine 2012, 7:1415-1422

Published Date: 12 March 2012

Readers of this article also read:

Green synthesis of water-soluble nontoxic polymeric nanocomposites containing silver nanoparticles

Prozorova GF, Pozdnyakov AS, Kuznetsova NP, Korzhova SA, Emel’yanov AI, Ermakova TG, Fadeeva TV, Sosedova LM

International Journal of Nanomedicine 2014, 9:1883-1889

Published Date: 16 April 2014

Methacrylic-based nanogels for the pH-sensitive delivery of 5-Fluorouracil in the colon

Ashwanikumar N, Kumar NA, Nair SA, Kumar GS

International Journal of Nanomedicine 2012, 7:5769-5779

Published Date: 15 November 2012

A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone

Hu Z, Liao M, Chen Y, Cai Y, Meng L, Liu Y, Lv N, Liu Z, Yuan W

International Journal of Nanomedicine 2012, 7:5719-5724

Published Date: 12 November 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Crystallization after intravitreal ganciclovir injection

Pitipol Choopong, Nattaporn Tesavibul, Nattawut Rodanant

Clinical Ophthalmology 2010, 4:709-711

Published Date: 14 July 2010