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Apoptosis inducing ability of silver decorated highly reduced graphene oxide nanocomposites in A549 lung cancer

Authors Khan M, Khan M, Al-Marri A, Al-Warthan A, Alkhathlan HZ, Siddiqui MRH, Nayak V, Kamal A, Adil SF

Received 20 November 2015

Accepted for publication 29 December 2015

Published 7 March 2016 Volume 2016:11 Pages 873—883

DOI https://doi.org/10.2147/IJN.S100903

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Thomas J. Webster

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Merajuddin Khan,1 Mujeeb Khan,1 Abdulhadi H Al-Marri,1 Abdulrahman Al-Warthan,1 Hamad Z Alkhathlan,1 Mohammed Rafiq H Siddiqui,1 Vadithe Lakshma Nayak,2 Ahmed Kamal,2 Syed F Adil1

1Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Department of Medicinal Chemistry and Pharmacology, CSIR – Indian Institute of Chemical Technology, Hyderabad, India

Abstract: Recently, graphene and graphene-based materials have been increasingly used for various biological applications due to their extraordinary physicochemical properties. Here, we demonstrate the anticancer properties and apoptosis-inducing ability of silver doped highly reduced graphene oxide nanocomposites synthesized by employing green approach. These nano­composites (PGE-HRG-Ag) were synthesized by using Pulicaria glutinosa extract (PGE) as a reducing agent and were evaluated for their anticancer properties against various human cancer cell lines with tamoxifen as the reference drug. A correlation between the amount of Ag nanoparticles on the surface of highly reduced graphene oxide (HRG) and the anticancer activity of nanocomposite was observed, wherein an increase in the concentration of Ag nanoparticles on the surface of HRG led to the enhanced anticancer activity of the nanocomposite. The nanocomposite PGE-HRG-Ag-2 exhibited more potent cytotoxicity than standard drug in A549 cells, a human lung cancer cell line. A detailed investigation was undertaken and Fluorescence activated cell sorting (FACS) analysis demonstrated that the nanocomposite PGE-HRG-Ag-2 showed G0/G1 phase cell cycle arrest and induced apoptosis in A549 cells. Studies such as, measurement of mitochondrial membrane potential, generation of reactive oxygen species (ROS) and Annexin V-FITC staining assay suggested that this compound induced apoptosis in human lung cancer cells.

Keywords: plant extract, graphene/silver nanocomposites, anticancer, apoptosis

Introduction

The emergence of multi-drug resistance in different diseases has raised the challenge of their treatment to a newer level and has led to more focused research endeavors toward finding newer agents with the desired biological properties. Among them, the research on the cancer treatment has been more emphasized due to the growing number of diagnosed cases as well as mortality, with cancer being the second leading cause of death among patients throughout the world.1 Apoptosis, the programmed cell death, is an important process for the normal functioning of cells. In cancer, the mutated cells go unregulated by apoptosis and result in unchecked cell multiplication. Agents inducing or promoting apoptotic cell death are one of the important cornerstones in anticancer drug development.

Natural product-based organic molecules are one of the major sources of anticancer drug development2,3 and in recent years the conjugation of two pharmacophoric units has shown promising results. However, the medicinal use of metal-containing ointments/drugs is also very popular and known to humans since ages as described in the Papyus Ebers in ~1550 BC.4 Coordination complex of ligands to a metal is reported to provide numerous advantages, such as facilitating the sustained release of the ligands in the body resulting in longer-acting drugs5 and improving the bioactivity of the ligands.6,7 Cisplatin, carboplatin, and oxaliplatin are a few renowned examples from this class as shown in Figure 1.8

Figure 1 Platinum (Pt)-containing anticancer drugs.

Recently, it has been observed that there is a significant shift toward employing graphene-based nanocomposites in biological applications wherein they have been used as antibacterial agents and antifungal agents.911 Additionally, it has also been reported to be employed in anticancer drug development as drug-carrier agents,10,1214 for sustained release of drugs. For instance, in several studies on the delivery of the anticancer drug doxorubicin, it has been revealed that graphene oxide (GRO) played an effective role in the delivery and release of the drug into the targeted cancer cell.15,16

Apart from this, several other studies have also been published regarding the applications of graphene and graphene-based materials for various biological purposes due to their extraordinary physicochemical properties, such as mechanical properties, electrical properties, electrochemical properties, and thermal conductivity.14,1724 However, despite the recent surge in such studies, a lot more needs to be done in order to fully exploit the potential of graphene-based materials in biological applications.2527 Among the various challenges, the biocompatibility and toxicity of graphene-based materials both at in vitro and in vivo levels is one of the major challenges.17 Several factors define the toxicity of these materials one of them is the use of hazardous chemicals employed in the synthesis of graphene-based materials, which needs special attention14 towards their removal procedures for post-synthesis purification, which is one of the most important factors to conclude the biocompatibility of these materials.

Commonly, graphene-based materials are prepared by chemical methods in which the reagents involved in the preparation and functionalization of these materials are hazardous and harmful to both environment and human well-being due to their high toxicity.28 Additionally, trace amounts of toxic-reducing agents present on the surface of these materials could seriously affect the potential properties of highly reduced graphene oxide (HRG) and have undesirable effects on its biological applications.16 Whereas, the green synthesis of graphene-based materials involves biocompatible ingredients under physiological conditions of temperature and pressure.29,30 Among various methods, plant extract (PE)-based syntheses of graphene and graphene-based materials have attracted significant attention due to their bulk availability, biocompatibility, and low cost.31 Furthermore, the biologically active molecules employed in the synthesis of nanomaterials, particularly phytomolecules, which exist in the PE, often play the role of functionalizing ligands, thereby impeding the usage of any external chemical stabilizers.32

In our previous study, we have demonstrated a facile, single-step green approach for the synthesis of graphene/Ag nanocomposites (PGE-HRG-Ag) via in situ reduction of both GRO and silver ions using Pulicaria glutinosa extract (PGE) as a reducing agent.33 The phytomolecules of the PGE not only functionalize the surface of the HRG but also facilitate conjugation of Ag nanoparticles (NPs) on the surface of HRG. In order to demonstrate the significance of the green synthesized PGE-HRG-Ag nanocomposite in the biological applications and in continuation of our studies toward development of more potent anticancer agents,3437 we investigated the in vitro anticancer activity of PGE-HRG-Ag nanocomposites (Figure 2). The anticancer evaluation studies of the as-prepared PGE-HRG-Ag nanocomposites were performed against various human cancer cell lines, including MCF-7 (breast), A549 (lung), Hela (cervical), DU-145 (prostate), and HepG2 (liver) using MTT study, while tamoxifen was used as the reference drug. Moreover, to the best of our knowledge, the anticancer properties of graphene/silver nanocomposite are being investigated for the first time.

Figure 2 Graphical representation of the green synthesis of graphene/silver (PGE-HRG-Ag) nanocomposites using Pulicaria glutinosa plant extract and their anticancer activities against various human cancer cell lines.
Abbreviations: T, temperature, t, time.

Experimental section

Materials

Graphite (99.999%, -200 mesh) was purchased from (Alfa Aesar, Ward Hill, MA, USA). Concentrated sulfuric acid (H2SO4, molecular weight 98.079 [98%]), potassium permanganate (KMnO4, molecular weight 158.034 [99%]), sodium nitrate (NaNO3, molecular weight 84.9947 [99%]), hydrogen peroxide (H2O2, molecular weight 34.0147 [30 wt%]), and all other solvents were purchased from (Aldrich chemicals, St Louis, MO, USA).

P. glutinosa, a wild plant growing in the hilly regions of Al-Hair in the central part of Kingdom of Saudi Arabia, was collected during March 2011, which was recognized and ascertained by a plant taxonomist from the Herbarium Division, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia. The details of the plant and preparation of the PE are given elsewhere.38 The PE solution, employed for the reduction of GRO, was made using 0.1 g of PE dissolved in 1 mL of solvent.

Synthesis and characterization of HRG/Ag nanocomposites (PGE-HRG-Ag)

Graphite oxide (GO) was synthesized from graphite powder by a modified Hummers method.39,40 The as-prepared GO was further treated to obtain the desired nanocomposites by varying the concentration of silver NPs. To begin with, 1 g of natural graphite and 0.875 g of NaNO3 were taken in a three-neck round bottom flask. To this mixture, 150 mL of H2SO4 was added gently. The mixture was kept stirred for the next 2 hours in an ice-water bath; subsequently, 4.5 g of KMnO4 was added slowly in a period of 2 hours with constant stirring. The resulting mixture was allowed to stir for the next 5 days at room temperature. Afterwards, 100 mL of 5 wt% H2SO4 aqueous solution was gradually added in 1 hour and the mixture was allowed to stir for another 2 hours. Thereafter, 3 g of 30 wt% aqueous solution of H2O2 was added, and the mixture was again left for stirring for another 2 hours. Finally, the resulting solution was washed with an aqueous solution of 3 wt% H2SO4 and 0.5 wt% H2O2 several times. A similar process was repeated with deionized water (DI) for three more times. The resulting mixture was dispersed in DI water and centrifuged for 2 hours at 9,000× g. The dispersion was washed extensively (~20 times) with DI water until a homogeneous brown-black dispersion was obtained.33

Synthesis of HRG/Ag nanocomposites (PGE-HRG-Ag)

GRO required for the preparation of PGE-HRG-Ag was obtained by dispersing 170 mg of as-prepared GO in 50 mL of distilled water under constant sonication for 30 minutes. The graphene/silver nanocomposite with 50 wt% of graphene (PGE-HRG-Ag-1) was prepared by taking 170 mg aqueous dispersion of GRO and 0.5 mM (84.93 mg) of AgNO3. The reaction mixture was prepared by taking 50 mL aqueous dispersion of GRO in a 250 mL round bottom flask. To this dispersion, separately prepared 50 mL aqueous solution of AgNO3 was added. The reaction mixture was allowed to stir for several minutes (~10 minutes), and subsequently, to this mixture, 10 mL aqueous solution of P. glutinosa PE was added. The resulting mixture was heated up to 90°C and was kept under stirring for 24 hours. Afterwards, the mixture was allowed to cool down and washed several times (three to four times) with DI. Finally, ~180 mg of black powder was obtained after centrifugation. PGE-HRG was also prepared by a similar method without using AgNO3.

Characterization

The formation of as-synthesized GO and PGE-HRG-Ag was confirmed by several techniques, including, UV-Vis spectroscopy (PerkinElmer lambda 35 [Waltham, MA, USA]), high-resolution transmission electron microscopy (JEM 1101 [JEOL, Tokyo, Japan]), XRD (D2 Phaser X-ray diffractometer [Bruker Optik GmbH, Ettlingen, Germany]), Cu Kα radiation (λ=1.5418 Å), and Raman spectroscopy (Renishaw, Gloucestershire, UK) equipped with a 514.5 nm line of argon ion laser as excitation source.

Anticancer activity

Nanocomposites (PGE-HRG-Ag-1 and -2) were evaluated for their anticancer activity in a panel of five human cancer cell lines, namely, MCF-7 (breast), A549 (lung), Hela (cervical), DU-145 (prostate), and HepG2 (liver) using MTT method,41 in which tamoxifen was used as a reference drug. The results are summarized in Table 1 and expressed as IC50 values. The studies were carried out on the cell lines procured from NCCS, Pune-India. The Indian Institute of Chemical Technology, Hyderabad, India, waived the requirement to obtain formal ethical approval for commercial cell lines.

Table 1 IC50 valuesa (in μg) for compounds PGE-HRG-Ag-1 and -2 in human cancer cell lines
Notes: a50% inhibitory concentration after 48 hours of drug treatment. bHuman breast cancer. cHuman cervical cancer. dHuman liver cancer. eHuman lung cancer. fHuman prostate cancer.

Cell cycle analysis

FACS experiment was done to evaluate the cell cycle analysis. In this experiment, cells (A549) were treated with nanocomposite PGE-HRG-Ag-2 at concentrations of 5 and 10 μg. After treatment, cells were harvested and washed with phosphate-buffered saline (PBS). The pellets were fixed with ice-cold 70% ethanol followed by staining with propidium iodide (Sigma-Aldrich Co., St Louis, MO, USA). Flow cytometry was performed using Becton Dickinson FACS Caliber instrument (Becton, Dickinson and Company, Franklin Lakes, NJ, USA).42

Measurement of ΔΨm

Cells (A549) were cultured and seeded in 6-well plates. After plating, cells were treated with nanocomposite PGE-HRG-Ag-2 at 5 and 10 μg concentrations for 48 hours. Cells were collected and washed with PBS. The washed cells were stained with JC-1 (5 μg/mL) and incubated at 37°C for 15 minutes. After incubation with JC-1, cells were then subjected to flow cytometric analysis on a flow cytometer (Becton Dickinson) in the FL1 and FL2 channels to detect mitochondrial potential.43

Effect on intracellular ROS generation

Reactive oxygen species (ROS) was measured by flow cytometry using 2′,7′dichlorofluorescindiacetate (DCFDA) as previously described.44 A549 cells were treated with nanocomposite PGE-HRG-Ag-2 at 5 and 10 μg concentrations for 48 hours. The treated cells were stained with DCFDA (2 μM) at 37°C for 30 minutes and then measured with the flow cytometer (FACS).

Annexin V-FITC for apoptosis

An amount of 1×106 A549 cells was seeded in 6-well plates followed by treatment with nanocomposite PGE-HRG-Ag-2 at 5 and 10 μg concentrations. Afterwards, the cells were washed with PBS at 3,000 rpm, and then flow cytometry was performed using Annexin-V-FITC apoptosis detection kit (Sigma-Aldrich) as described earlier.45

Results and discussion

The phytomolecule-rich PE of P. glutinosa when added to a dispersion of GRO and AgNO3 reduces both Ag ions and GRO simultaneously resulting in the formation of PGE-HRG-Ag nanocomposites. By varying the concentration of AgNO3, two different samples of PGE-HRG-Ag nanocomposites, namely, PGE-HRG-Ag-1 and -2 were prepared using 0.5 and 1 mmol of AgNO3, respectively, and the effect of this concentration on the anticancer and apoptosis-inducing ability of the nanocomposites was studied. The formation of these nanocomposites was examined by Ultraviolet–visible spectroscopy (UV-Vis) and the nanocomposites were subjected to an X-ray powder diffraction (XRD) analysis to confirm their crystalline nature (Figure 3). The characteristic reflections in the diffractogram suggested the complete reduction of GO employing PE. The samples were further characterized by Raman spectroscopy. The PGE-HRG without Ag NPs exhibits two Raman signals at 1,582 and 1,343 cm−1, respectively, as shown in Figure 4. These signals which correspond to the G and D bands, respectively, also appeared at a similar position with weak intensities in PGE-HRG-Ag. However, the intensities of these signals were significantly enhanced by the incorporation of Ag NPs, which confirms the binding of silver NPs to the surface of graphene. Moreover, the transmission electron microscopy images of the nanocomposites PGE-HRG-Ag-1 and -2 clearly indicated that the Ag NP density can be amplified by increasing the amount of AgNO3 and the particle size distribution was found to be more uniform for higher concentrations (Figure 5).

Figure 3 (A) UV-Vis absorption spectra of GRO, PGE-HRG, and PGE-HRG-Ag; (B) XRD spectra of GRO, PGE-HRG, and PGE-HRG-Ag nanocomposites.
Abbreviation: GRO, graphene oxide.

Figure 4 Raman spectra of graphene/silver nanocomposites with 50 wt% (PGE-HRG-Ag-1, pink line) and 100 wt% (PGE-HRG-Ag-2, green line) of Ag NPs with respect to graphene and PGE-HRG without Ag NPs (blue line).
Notes: Increasing the concentration of Ag NPs, the intensities of the Raman signals also increase, which not only confirms the binding of the Ag NPs on the surface of graphene but also demonstrates the increased density of the Ag NPs.
Abbreviation: NPs, nanoparticles.

Figure 5 High resolution TEM images of (A) PGE-HRG-Ag-1 and (B) PGE-HRG-Ag-2 prepared using 0.5 and 1 mmol of AgNO3, respectively.
Abbreviation: TEM, transmission electron microscopy.

Anticancer activity

Apoptosis is a form of cell death in which a programmed sequence of events eliminates cells without damaging the neighboring cells. Apoptosis performs a key role in sustaining normal tissue homeostasis by regulating the balance between cell proliferation and cell death.46 The design and development of new molecules that can induce apoptosis in cancer cells is an attractive approach for the treatment of cancer. The potential biomedical applications of graphene and graphene-based nanocomposites, such as graphene/silver nanocomposites, have been exploited for several applications, including antibacterial agents and nanocarriers for controlled loading and targeted delivery of anticancer drugs. However, the anticancer properties of graphene based-inorganic NP nanocomposites have been rarely tested, such as the one reported by Gurunathan et al47 wherein, they have reportedly used rGO–Ag nanocomposite against human ovarian cancer cells A2780. Hence, in this study, newly synthesized nanocomposites (PGE-HRG-Ag-1 and PGE-HRG-Ag-2) were evaluated for anticancer activity and their apoptosis inducing ability.

The as prepared nanocomposites (PGE-HRG-Ag-1 and PGE-HRG-Ag-2) with varying concentration of Ag NPs were evaluated for their anticancer activity in a panel of five human cancer cell lines, namely MCF-7 (breast), A549 (lung), Hela (cervical), DU-145 (prostate) and HepG2 (liver) by using MTT method,41 and Tamoxifen was used as the reference drug. The results are given in Table 1 and expressed as IC50 values. The results exhibited that these nanocomposites demonstrate promising anticancer activity against the tested cell lines and PGE-HRG-Ag-2 exhibited more potent activity than standard tamoxifen against the human lung cancer cell line, A549, with an IC50 value of 9.63 μg. Therefore, A549 was selected as a model cell line for subsequent experiments.

Cell cycle analysis

Several anticancer compounds demonstrate their growth inhibitory effect either by arresting the cell cycle at a specific checkpoint of cell cycle or by induction of apoptosis or a collective effect of both cycle block and apoptosis.48,49 The in vitro screening results exhibited that the nanocomposite PGE-HRG-Ag-2 showed significant activity against the human lung cancer cell line, A549. Therefore, it was considered of interest to understand whether this inhibition of cell growth was on account of cell cycle arrest or induction of apoptosis or both. Hence, in this study, A549 cells were treated with this nanocomposite at concentrations of 5 and 10 μg for 48 hours. The data obtained clearly indicated that this nanocomposite arrested cell cycle at G0/G1 phase. The values obtained are given in Table 2. Interestingly, when the concentration was increased from 5 to 10 μg, it was noticed that the percentage of cells in G0/G1 phase was decreased and accumulation of cells in subG1 phase increased in a dose-dependent manner, which indicates the onset of apoptosis (Figure 6).50

Table 2 FACS analysis in A549 human lung cancer cell line after treatment with PGE-HRG-Ag-2
Abbreviation: FACS, fluorescence-activated cell sorting.

Figure 6 FACS analysis in A549 human lung cancer cell line after treatment with PGE-HRG-Ag-2.
Notes: (A) Untreated control cells (A549), (B) PGE-HRG-Ag-2 (5 μg), and (C) PGE-HRG-Ag-2 (10 μg).
Abbreviation: FACS, fluorescence-activated cell sorting.

Measurement of ΔΨm

Mitochondrial membrane potential (ΔΨm) plays significant role for mitochondrial integrity and bio energetic function.43 Mitochondrial changes, including loss of ΔΨm, are key events that take place during drug-induced apoptosis. Mitochondrial injury by PE-HRG-Ag-2 was evaluated by detecting drops in ΔΨm. In this experiment, we have investigated the involvement of mitochondria in the induction of apoptosis by PE-HRG-Ag-2. After 48 hours of drug treatment with this compound at 5 and 10 μg concentrations, it was observed that there was a reduced ΔΨm of A549 cells, assessed by JC-1 staining (Figure 7).

Figure 7 Drops in membrane potential (ΔΨm) were assessed by JC-1 staining of MCF-7 cells treated with compound PGE-HRG-Ag-2 and samples were then subjected to flow cytometry analysis on a FACScan (Becton Dickinson).
Notes: (A) Untreated control cells (A549), (B) PGE-HRG-Ag-2 (5 μg), and (C) PGE-HRG-Ag-2 (10 μg).
Abbreviations: FACS, fluorescence-activated cell sorting; ΔΨm, mitochondrial membrane potential; Q1-UL, quadrant 1-upper left; Q1-UR, quadrant 1-upper right; Q1-LL, quadrant 1-lower left; Q1-LR, quadrant 1-lower right.

Effect on intracellular ROS generation

A number of anticancer compounds have been known to exert their anticancer effects by the generation of ROS,52,53 which is considered as one of the key mediators of apoptotic signaling. In this connection, nanocomposite PGE-HRG-Ag-2 was evaluated for its ROS-inducing capability in A549 cells. A549 cells were treated with this compound and stained with DCFDA for ROS measurement. After treatment with this nanocomposite at 5 and 10 μg concentrations for 48 hours, the level of ROS was significantly increased (Figure 8). The ratio of DCF-positive cells for PGE-HRG-Ag-2 was 23.2% and 32.9% at 5 and 10 μg concentrations respectively, for 48 hours and the level of ROS was significantly increased (Figure 8). Hence, from the test results it can be concluded that the compounds tested had enhanced the generation of ROS in A549 cells.

Figure 8 The effect of PGE-HRG-Ag-2 on the ROS production in human lung cancer (A549).
Notes: (A) Untreated control cells (A549), (B) PGE-HRG-Ag-2 (5 μg), and (C) PGE-HRG-Ag-2 (10 μg).
Abbreviation: ROS, reactive oxygen species.

Annexin V-FITC for apoptosis

The apoptotic effect of compound PGE-HRG-Ag-2 was studied by Annexin V-FITC assay.54 In this study, A549 cells were treated with nanocomposite PGE-HRG-Ag-2 for 48 hours at 5 and 10 μg concentrations to examine the apoptotic effect. Results, as shown in Table 3, suggest that this compound induced significant apoptosis in human lung cancer cells.A graphical representation of the data is given in Figure 9.

Table 3 Data obtained from Annexin V-FITC staining assay
Abbreviations: UL, necrotic cells; UR, late apoptotic cells; LL, live cells; LR, early apoptotic cells.

Figure 9 Annexin V-FITC staining.
Notes: (A) Untreated control cells (A549), (B) PGE-HRG-Ag-2 (5 μg), and (C) PGE-HRG-Ag-2 (10 μg).
Abbreviations: Q1-UL, quadrant 1-upper Left; Q1-UR, quadrant 1-upper right; Q1-LL, quadrant 1-lower left; Q1-LR, quadrant 1-lower right.

Conclusion

Development of anticancer agents that can induce apoptosis is an attractive target for cancer cure and treatment. In this study, we have investigated the anticancer property of green synthesized nanocomposites of silver decorated highly reduced graphene oxide (PGE-HRG-Ag-1 & 2). Flow cytometry results suggested that this compound arrested the cell cycle at G0/G1 phase at 5 μg concentration. Interestingly, when the concentration was increased from 5 to 10 μg, it was observed that the percentage of cells in G0/G1 phase was decreased and accumulation of cells in subG1 phase increased in a dose-dependent manner, which indicates the onset of apoptosis. From the studies such as the measurement of ΔΨm, generation of ROS, and Annexin V-FITC assay it can be concluded that the tested compound induced cell death by apoptosis and PGE-HRG-Ag-2 was found to be the most potent in inducing cell death by apoptosis against human lung cancer cells, A549.

Acknowledgment

This project was supported by King Saud University, Deanship of Scientific Research, College of Science, Research Centre.

Disclosure

The authors report no conflicts of interest in this work.


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