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The homopurine–homopyrimidine DNA sequence flanking the thyroglobulin gene does not behave as a transcriptional diode in living cells

Authors Christophe-Hobertus C, Christophe D

Received 25 March 2015

Accepted for publication 18 May 2015

Published 27 July 2015 Volume 2015:6 Pages 107—115

DOI https://doi.org/10.2147/RRB.S84344

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 3

Editor who approved publication: Professor Zvi Kelman

Christiane Christophe-Hobertus, Daniel Christophe

Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine, Université Libre de Bruxelles, Gosselies, Belgium

Abstract: Homopurine–homopyrimidine DNA sequences are found at higher than expected frequency around gene promoters in mammalian genomes. They were reported to act as a transcriptional diode in in vitro assays, as only the homopurine DNA strand was transcribed. We show here that the homopurine–homopyrimidine upstream element of the thyroglobulin gene promoter is able to reduce transcription initiated from different promoters, both on transiently transfected plasmids or when integrated into the genome of rat thyroid cells. In living cells, this homopurine–homopyrimidine sequence inhibits transcription of both DNA strands.

Keywords: Pu/Py, promoter, transcription, thyroglobulin, thyroid

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