Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Authors Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L

Received 28 April 2015

Accepted for publication 3 August 2015

Published 20 November 2015 Volume 2015:10(1) Pages 7145—7163


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Sunny Kumar

Peer reviewer comments 2

Editor who approved publication: Dr Thomas J Webster

Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1

1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic

Abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

Keywords: nanostructure, titanium nanotubes, cell adhesion, Saos-2 cells, osteogenic differentiation

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

Green synthesis of silver nanoparticles using cranberry powder aqueous extract: characterization and antimicrobial properties

Ashour AA, Raafat D, El-Gowelli HM, El-Kamel AH

International Journal of Nanomedicine 2015, 10:7207-7221

Published Date: 1 December 2015

Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant

Binyamin O, Larush L, Frid K, Keller G, Friedman-Levi Y, Ovadia H, Abramsky O, Magdassi S, Gabizon R

International Journal of Nanomedicine 2015, 10:7165-7174

Published Date: 20 November 2015

Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Kastrinaki G, Samsouris C, Kosmidis EK, Papaioannou E, Konstandopoulos AG, Theophilidis G

International Journal of Nanomedicine 2015, 10:7089-7096

Published Date: 19 November 2015

The effects of hierarchical micro/nanosurfaces decorated with TiO2 nanotubes on the bioactivity of titanium implants in vitro and in vivo

Ding XL, Zhou L, Wang JX, Zhao QX, Lin X, Gao Y, Li SB, Wu JY, Rong MD, Guo ZH, Lai CH, Lu HB, Jia F

International Journal of Nanomedicine 2015, 10:6955-6973

Published Date: 9 November 2015

Visible-light-responsive ZnCuO nanoparticles: benign photodynamic killers of infectious protozoans

Nadhman A, Nazir S, Khan MI, Ayub A, Muhammad B, Khan M, Shams DF, Yasinzai M

International Journal of Nanomedicine 2015, 10:6891-6903

Published Date: 4 November 2015

Improvement in the amine glass platform by bubbling method for a DNA microarray

Jee SH, Kim JW, Lee JH, Yoon YS

International Journal of Nanomedicine 2015, 10:273-280

Published Date: 3 October 2015

Nanoparticles of barium induce apoptosis in human phagocytes

Mores L, França EL, Silva NA, Suchara EA, Honorio-França AC

International Journal of Nanomedicine 2015, 10:6021-6026

Published Date: 28 September 2015

Inorganic nanolayers: structure, preparation, and biomedical applications

Saifullah B, Hussein MZB

International Journal of Nanomedicine 2015, 10:5609-5633

Published Date: 2 September 2015