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The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Authors Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L

Received 28 April 2015

Accepted for publication 3 August 2015

Published 20 November 2015 Volume 2015:10(1) Pages 7145—7163

DOI https://doi.org/10.2147/IJN.S87474

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Sunny Kumar

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Thomas J Webster

Elena Filova,1 Jaroslav Fojt,2 Marketa Kryslova,1 Hynek Moravec,2 Ludek Joska,2 Lucie Bacakova1

1Department of Biomaterials and Tissue Engineering, Institute of Physiology, Czech Academy of Sciences, 2Department of Metals and Corrosion Engineering, University of Chemistry and Technology, Prague, Czech Republic

Abstract: Ti-6Al-4V-based nanotubes were prepared on a Ti-6Al-4V surface by anodic oxidation on 10 V, 20 V, and 30 V samples. The 10 V, 20 V, and 30 V samples and a control smooth Ti-6Al-4V sample were evaluated in terms of their chemical composition, diameter distribution, and cellular response. The surfaces of the 10 V, 20 V, and 30 V samples consisted of nanotubes of a relatively wide range of diameters that increased with the voltage. Saos-2 cells had a similar initial adhesion on all nanotube samples to the control Ti-6Al-4V sample, but it was lower than on glass. On day 3, the highest concentrations of both vinculin and talin measured by enzyme-linked immunosorbent assay and intensity of immunofluorescence staining were on 30 V nanotubes. On the other hand, the highest concentrations of ALP, type I collagen, and osteopontin were found on 10 V and 20 V samples. The final cellular densities on 10 V, 20 V, and 30 V samples were higher than on glass. Therefore, the controlled anodization of Ti-6Al-4V seems to be a useful tool for preparing nanostructured materials with desirable biological properties.

Keywords: nanostructure, titanium nanotubes, cell adhesion, Saos-2 cells, osteogenic differentiation

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