The Association Between Interleukin 1 Beta Promoter Polymorphisms And Keratoconus Incidence And Severity In An Egyptian Population
Received 25 June 2019
Accepted for publication 27 August 2019
Published 14 November 2019 Volume 2019:13 Pages 2217—2223
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Melinda Thomas
Peer reviewer comments 2
Editor who approved publication: Dr Scott Fraser
Karim Mahmoud Nabil,1 Ghada Mohamed Elhady,2 Heba Morsy2
1Department of Ophthalmology, Faculty of Medicine, University of Alexandria, Alexandria, Egypt; 2Department of Human Genetics, Medical Research Institute, University of Alexandria, Alexandria, Egypt
Correspondence: Karim Mahmoud Nabil
Department of Ophthalmology, Faculty of Medicine, University of Alexandria, Sultan Hussein Street, Khartoum Square, Alexandria 21523, Egypt
Purpose: In the present study, we investigated whether interleukin 1 beta (IL1B) promoter polymorphisms are associated with keratoconus in an Egyptian population and their association with disease severity.
Methods: A total of 95 Egyptian keratoconus patients and 126 Egyptian healthy controls were enrolled in the study. Two IL1B single nucleotide polymorphisms (SNPs) (rs1143627 and rs16944) were genotyped using Taqman real-time PCR to compare haplotype, genotype, and allele frequencies between cases and controls (primary outcome) and their association with disease severity (secondary outcome).
Results: Statistically significant association was observed for rs1143627 and rs16944; the T allele of rs1143627 and the G allele of rs16944 were associated with an increased risk of keratoconus (p < 0.001, odds ratio = 3.313, 4.770, respectively). The TT genotype of rs1143627 and the GG genotype of rs16944 were strongly associated with an increased risk of keratoconus (p < 0.001, odds ratio = 5.631, 11.478, respectively). The G allele of rs16944 was associated with an increased curvature of the flattest corneal meridian Kf in keratoconus (p = 0.041). The GG genotype of rs16944 was associated with an increased curvature of the flattest corneal meridian Kf, steepest corneal meridian Ks and average corneal curvature Kavg in keratoconus (p = 0.01, 0.046, 0.023, respectively).
Conclusion: IL1B is suspected to play a crucial role, both in development and severity of keratoconus in Egyptian population.
Keywords: interleukin 1 beta, promoter polymorphisms, keratoconus, Egypt
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