Supercritical carbon dioxide-developed silk fibroin nanoplatform for smart colon cancer therapy
Authors Xie MB, Fan DJ, Li Y, He XW, Chen XM, Chen YF, Zhu JX, Xu GB, Wu XJ, Lan P
Received 28 June 2017
Accepted for publication 29 August 2017
Published 20 October 2017 Volume 2017:12 Pages 7751—7761
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 2
Editor who approved publication: Prof. Dr. Dongwoo Khang
Maobin Xie,1,2,* Dejun Fan,3,* Yi Li,4 Xiaowen He,3 Xiaoming Chen,1,2 Yufeng Chen,3 Jixiang Zhu,1,2 Guibin Xu,5 Xiaojian Wu,3 Ping Lan3
1School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China; 2Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, China;3Department of Colorectal Surgery, Sixth Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China; 4School of Materials, University of Manchester, Manchester, UK; 5Department of Urology, Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, China
*These authors contributed equally to this work
Purpose: To deliver insoluble natural compounds into colon cancer cells in a controlled fashion.
Materials and methods: Curcumin (CM)–silk fibroin (SF) nanoparticles (NPs) were prepared by solution-enhanced dispersion by supercritical CO2 (SEDS) (20 MPa pressure, 1:2 CM:SF ratio, 1% concentration), and their physicochemical properties, intracellular uptake efficiency, in vitro anticancer effect, toxicity, and mechanisms were evaluated and analyzed.
Results: CM-SF NPs (<100 nm) with controllable particle size were prepared by SEDS. CM-SF NPs had a time-dependent intracellular uptake ability, which led to an improved inhibition effect on colon cancer cells. Interestingly, the anticancer effect of CM-SF NPs was improved, while the side effect on normal human colon mucosal epithelial cells was reduced by a concentration of ~10 µg/mL. The anticancer mechanism involves cell-cycle arrest in the G0/G1 and G2/M phases in association with inducing apoptotic cells.
Conclusion: The natural compound-loaded SF nanoplatform prepared by SEDS indicates promising colon cancer-therapy potential.
Keywords: supercritical, silk fibroin, nanoparticle, natural compounds, colon cancer
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]