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Protection of silver nanoparticles using Eysenhardtia polystachya in peroxide-induced pancreatic β-Cell damage and their antidiabetic properties in zebrafish

Authors Garcia Campoy AH, Perez Gutierrez RM, Manriquez-Alvirde G, Muñiz Ramirez A

Received 25 January 2018

Accepted for publication 3 March 2018

Published 1 May 2018 Volume 2018:13 Pages 2601—2612

DOI https://doi.org/10.2147/IJN.S163714

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Mohankandhasamy Ramasamy

Peer reviewer comments 2

Editor who approved publication: Dr Thomas Webster


Abraham Heriberto Garcia Campoy,1 Rosa Martha Perez Gutierrez,1 Gabriela Manriquez-Alvirde,2 Alethia Muñiz Ramirez3

1Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingenieria Quimica e Industrias extractivas IPN, Unidad Profesional Adolfo Lopez Mateos, Mexico City, Mexico; 2Universidad Autonoma Metropolitana, Mexico City, Mexico; 3CONACYT-IPICYT/CIIDZA, San Luis Potosí, México

Background: The aim was to explore the efficacy of extract of Eysenhardtia polystachya-loaded silver nanoparticles (EP/AgNPs) on pancreatic β cells, INS-1 cells, and zebrafish as a valuable model for the study of diabetes mellitus.
Materials and methods: EP/AgNPs were synthesized using methanol/water bark extract of E. polystachya and characterized using various physicochemical techniques.
Results: Immersion of adult zebrafish in 111 mM glucose solution resulted in a sustained hyperglycemic, hyperlipidemic state, and serum insulin levels decreased. The synthesized EP/AgNPs showed an absorption peak at 413 nm on ultraviolet–visible spectroscopy, revealing the surface plasmon resonance of the nanoparticles. Transmission electron microscopy indicated that most of the particles were spherical, with a diameter of 10–12 nm, a polydispersity index of 0.197, and a zeta potential of -32.25 mV, suggesting high stability of the nanoparticles. EP/AgNPs promote pancreatic β-cell survival, insulin secretion, enhanced hyperglycemia, and hyperlipidemia in glucose-induced diabetic zebrafish. EP/AgNPs also showed protection of the pancreatic β-cell line INS-1 against hydrogen peroxide-induced oxidative injury.
Conclusion: The results indicate that EP/AgNPs have good antidiabetic activity and therefore could be used to prevent the development of diabetes.

Keywords: Eysenhardtia polystachya, zebrafish, silver nanoparticles, diabetes, insulin, hyperlipidemia

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