Back to Browse Journals » International Journal of Nanomedicine » Volume 7

Portable microfluidic chip for detection of Escherichia coli in produce and blood

Authors Wang S, Inci F, Chaunzwa TL, Ramanujam A, Vasudevan A, Subramanian S, Ip AC, Sridharan B, Gurkan UA, Demirci U

Received 1 January 2012

Accepted for publication 24 January 2012

Published 29 May 2012 Volume 2012:7 Pages 2591—2600

DOI http://dx.doi.org/10.2147/IJN.S29629

Review by Single-blind

Peer reviewer comments 6

ShuQi Wang,1* Fatih Inci,1* Tafadzwa L Chaunzwa,1 Ajay Ramanujam,1 Aishwarya Vasudevan,1 Sathya Subramanian,1 Alexander Chi Fai Ip,1 Banupriya Sridharan,1 Umut Atakan Gurkan,1 Utkan Demirci,1,2
1
Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, 2Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA,

*These authors contributed equally to this work

Abstract: Pathogenic agents can lead to severe clinical outcomes such as food poisoning, infection of open wounds, particularly in burn injuries and sepsis. Rapid detection of these pathogens can monitor these infections in a timely manner improving clinical outcomes. Conventional bacterial detection methods, such as agar plate culture or polymerase chain reaction, are time-consuming and dependent on complex and expensive instruments, which are not suitable for point-of-care (POC) settings. Therefore, there is an unmet need to develop a simple, rapid method for detection of pathogens such as Escherichia coli. Here, we present an immunobased microchip technology that can rapidly detect and quantify bacterial presence in various sources including physiologically relevant buffer solution (phosphate buffered saline [PBS]), blood, milk, and spinach. The microchip showed reliable capture of E. coli in PBS with an efficiency of 71.8% ± 5% at concentrations ranging from 50 to 4,000 CFUs/mL via lipopolysaccharide binding protein. The limits of detection of the microchip for PBS, blood, milk, and spinach samples were 50, 50, 50, and 500 CFUs/mL, respectively. The presented technology can be broadly applied to other pathogens at the POC, enabling various applications including surveillance of food supply and monitoring of bacteriology in patients with burn wounds.

Keywords: Escherichia coli, microchip, sepsis, food safety, point-of-care

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF] View Full Text [HTML] 

 

Other article by this author:

Simple filter microchip for rapid separation of plasma and viruses from whole blood

Wang SQ, Sarenac D, Chen MH, Huang SH, Giguel FF, Kuritzkes DR, Demirci U

International Journal of Nanomedicine 2012, 7:5019-5028

Published Date: 17 September 2012

Readers of this article also read:

Development of a lauric acid/albumin hybrid iron oxide nanoparticle system with improved biocompatibility

Zaloga J, Janko C, Nowak J, Matuszak J, Knaup S, Eberbeck D, Tietze R, Unterweger H, Friedrich RP, Duerr S, Heimke-Brinck R, Baum E, Cicha I, Dörje F, Odenbach S, Lyer S, Lee G, Alexiou C

International Journal of Nanomedicine 2014, 9:4847-4866

Published Date: 20 October 2014

Hemorrhage in mouse tumors induced by dodecaborate cluster lipids intended for boron neutron capture therapy

Schaffran T, Jiang N, Bergmann M, Küstermann E, Süss R, Schubert R, Wagner FM, Awad D, Gabel D

International Journal of Nanomedicine 2014, 9:3583-3590

Published Date: 29 July 2014

Enhanced oral absorption and therapeutic effect of acetylpuerarin based on D-α-tocopheryl polyethylene glycol 1000 succinate nanoemulsions

Sun DQ, Wei XB, Xue X, Fang ZJ, Ren MR, Lou HY, Zhang XM

International Journal of Nanomedicine 2014, 9:3413-3423

Published Date: 18 July 2014

Rapid efficient synthesis and characterization of silver, gold, and bimetallic nanoparticles from the medicinal plant Plumbago zeylanica and their application in biofilm control

Salunke GR, Ghosh S, Santosh Kumar RJ, Khade S, Vashisth P, Kale T, Chopade S, Pruthi V, Kundu G, Bellare JR, Chopade BA

International Journal of Nanomedicine 2014, 9:2635-2653

Published Date: 27 May 2014

Degradable copolymer based on amphiphilic N-octyl-N-quatenary chitosan and low-molecular weight polyethylenimine for gene delivery

Liu CC, Zhu Q, Wu WH, Xu XL, Wang XY, Gao S, Liu KH

International Journal of Nanomedicine 2012, 7:5339-5350

Published Date: 8 October 2012

Simple filter microchip for rapid separation of plasma and viruses from whole blood

Wang SQ, Sarenac D, Chen MH, Huang SH, Giguel FF, Kuritzkes DR, Demirci U

International Journal of Nanomedicine 2012, 7:5019-5028

Published Date: 17 September 2012

PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering

Chen J, Zhou B, Li Q, Ouyang J, Kong J, Zhong W, Xing MM

International Journal of Nanomedicine 2011, 6:2533-2542

Published Date: 21 October 2011

Design, modeling, expression, and chemoselective PEGylation of a new nanosize cysteine analog of erythropoietin

Ahangari Cohan R, Madadkar-Sobhani A, Khanahmad H, Roohvand F, Aghasadeghi MR, Hedayati MH, Barghi Z, Shafiee Ardestani M, Nouri Inanlou D, Norouzian D

International Journal of Nanomedicine 2011, 6:1217-1227

Published Date: 15 June 2011