Back to Journals » International Journal of Nanomedicine » Volume 7

Nanocalcium-deficient hydroxyapatite–poly (ε-caprolactone)–polyethylene glycol–poly (ε-caprolactone) composite scaffolds

Authors Wang Z, Li M, Yu B, Cao L, Yang Q, Su J

Received 23 February 2012

Accepted for publication 27 March 2012

Published 10 July 2012 Volume 2012:7 Pages 3123—3131

DOI https://doi.org/10.2147/IJN.S31162

Review by Single-blind

Peer reviewer comments 4

Zhiwei Wang,* Ming Li,* Baoqing Yu, Liehu Cao, Qingsong Yang, Jiacan Su

Department of Orthopedics, Shanghai Hospital, Second Military Medical University, Shanghai, People's Republic of China

*These authors contributed equally to this work

Abstract: A bioactive composite of nano calcium-deficient apatite (n-CDAP) with an atom molar ratio of calcium to phosphate (Ca/P) of 1.50 and poly(ε-caprolactone)–poly(ethylene glycol)–poly(ε-caprolactone) (PCL–PEG–PCL) was synthesized, and a composite scaffold was fabricated. The composite scaffolds with 40 wt% n-CDAP contained well interconnected macropores around 400 µm, and exhibited a porosity of 75%. The weight-loss ratio of the n-CDAP/PCL–PEG–PCL was significantly greater than nano hydroxyapatite (n-HA, Ca/P = 1.67)/PCL–PEG–PCL composite scaffolds during soaking into phosphate-buffered saline (pH 7.4) for 70 days, indicating that n-CDAP-based composite had good degradability compared with n-HA. The viability ratio of MG-63 cells was significantly higher on n-CDAP than n-HA-based composite scaffolds at 3 and 5 days. In addition, the alkaline phosphatase activity of the MG-63 cells cultured on n-CDAP was higher than n-HA-based composite scaffolds at 7 days. Histological evaluation showed that the introduction of n-CDAP into PCL–PEG–PCL enhanced the efficiency of new bone formation when the composite scaffolds were implanted into rabbit bone defects. The results suggested that the n-CDAP-based composite exhibits good biocompatibility, biodegradation, and osteogenesis in vivo.

Keywords: nano calcium-deficient apatite, composite scaffold, degradability, cell responses, osteogenesis

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other article by this author:

Effects of hydrothermal treatment on the properties of nanoapatite crystals

Liang W, Niu Y, Ge S, Song S, Su J, Luo Z

International Journal of Nanomedicine 2012, 7:5151-5158

Published Date: 28 September 2012

Readers of this article also read:

Synthesis, characterization, and antimicrobial properties of copper nanoparticles

Usman MS, El Zowalaty ME, Shameli K, Zainuddin N, Salama M, Ibrahim NA

International Journal of Nanomedicine 2013, 8:4467-4479

Published Date: 21 November 2013

Biodistribution, kinetics, and biological fate of SPION microbubbles in the rat

Barrefelt A, Saghafian M, Kuiper R, Ye F, Egri G, Klickermann M, Brismar TB, Aspelin P, Muhammed M, Dähne L, Hassan M

International Journal of Nanomedicine 2013, 8:3241-3254

Published Date: 26 August 2013

Nucleocytoplasmic transport blockage by SV40 peptide-modified gold nanoparticles induces cellular autophagy

Tsai TL, Hou CC, Wang HC, Yang ZS, Yeh CS, Shieh DB, Su WC

International Journal of Nanomedicine 2012, 7:5215-5234

Published Date: 8 October 2012

Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Generalov R, Kavaliauskiene S, Westrøm S, Chen W, Kristensen S, Juzenas P

International Journal of Nanomedicine 2011, 6:1875-1888

Published Date: 7 September 2011

Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

Xu W, Ganz C, Weber U, Adam M, Holzhüter G, Wolter D, Frerich B, Vollmar B, Gerber T

International Journal of Nanomedicine 2011, 6:1543-1552

Published Date: 2 August 2011

Nanovaccine for leishmaniasis: preparation of chitosan nanoparticles containing Leishmania superoxide dismutase and evaluation of its immunogenicity in BALB/c mice

Danesh-Bahreini MA, Shokri J, Samiei A, Kamali-Sarvestani E, Barzegar-Jalali M, Mohammadi-Samani S

International Journal of Nanomedicine 2011, 6:835-842

Published Date: 20 April 2011

The preparation of 3,5-dihydroxy-4-isopropylstilbene nanoemulsion and in vitro release

Zhang Y, Gao JG, Zheng HT, Zhang R, Han YC

International Journal of Nanomedicine 2011, 6:649-657

Published Date: 1 April 2011

Preliminary biocompatible evaluation of nano-hydroxyapatite/polyamide 66 composite porous membrane

Yili Qu, Ping Wang, Yi Man, et al

International Journal of Nanomedicine 2010, 5:429-435

Published Date: 21 June 2010