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Loading BMP-2 on nanostructured hydroxyapatite microspheres for rapid bone regeneration

Authors Zhou PY, Wu JH, Xia Y, Yuan Y, Zhang HY, Xu SG, Lin KL

Received 28 November 2017

Accepted for publication 8 May 2018

Published 11 July 2018 Volume 2018:13 Pages 4083—4092

DOI https://doi.org/10.2147/IJN.S158280

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Mohankandhasamy Ramasamy

Peer reviewer comments 3

Editor who approved publication: Dr Linlin Sun


Panyu Zhou,1,* Jianghong Wu,1,* Yan Xia,1,* Ye Yuan,1 Hongyue Zhang,1 Shuogui Xu,1 Kaili Lin2

1Department of Emergency, Changhai Hospital, Second Military Medical University, Shanghai 200433, People’s Republic of China; 2Department of Oral & Cranio-Maxillofacial Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People’s Republic of China

*These authors contributed equally to this work

Introduction: Tissue engineering is a promising strategy for bone regeneration in repairing massive bone defects. The surface morphology of implanted materials plays a key role in bone healing; these materials incorporate osteoinductive factors to improve the efficiency of bone regeneration.
Materials and methods: In the current study, nanostructured hydroxyapatite (nHAp) microspheres were prepared via a hydrothermal transformation method using calcium silicate (CS) microspheres as precursors; the CS microspheres were obtained by a spray-drying method. The nHAp microspheres constructed by the nano-whiskers significantly improved the ability of the microspheres to adsorb the bioactive protein (BMP-2) and reduce its initial burst release. To evaluate the in vivo bone regeneration of microspheres, both conventional hydroxyapatite (HAp) and nHAp microspheres were either loaded with recombinant human bone morphogenetic protein-2 (rhBMP-2) or not loaded with the protein; these microspheres were implanted in rat femoral bone defects for 4 and 8 weeks.
Results and discussion: The results of our three-dimensional (3D) micro-computed tomography (CT) and histomorphometric observations showed that the combination of the nanostructured surface and rhBMP-2 obviously improved osteogenesis compared to conventional HAp microspheres loaded with rhBMP-2. Our results suggest that the nHAp microspheres with a nanostructured surface adsorb rhBMP-2 for rapid bone formation; they therefore show the potential to act as carriers in bone tissue regeneration.

Keywords: BMP-2, nanostructure, hydroxyapatite, hydrothermal transformation, bone regeneration

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