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Influence of MDR1 methylation on the curative effect of interventional embolism chemotherapy for cervical cancer

Authors Huang Z, Zhang S, Shen YP, Liu WX, Long JP, Zhou S

Received 31 August 2015

Accepted for publication 19 October 2015

Published 15 February 2016 Volume 2016:12 Pages 217—223

DOI https://doi.org/10.2147/TCRM.S95453

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Hoa Le

Peer reviewer comments 2

Editor who approved publication: Professor Deyun Wang

Zhi Huang,1,* Shuai Zhang,2,* Yaping Shen,1 Weixin Liu,1 Jipu Long,1 Shi Zhou1

1Department of Interventional Radiology, The Affiliated Baiyun Hospital of Guizhou Medical University, Guizhou Medical University, Guiyang, People’s Republic of China; 2Department of Interventional Radiology, Cancer Hospital of Guizhou Medical University, Guiyang, People’s Republic of China

*These authors contributed equally to this work


Background: Multi-drug resistance (MDR) is the main cause of tumor failure to chemotherapy. This study aims to explore the influence of MDR1 methylation on curative effect of interventional embolism chemotherapy for cervical cancer.
Methods: Sixty-seven patients with cervical cancer receiving embolism chemotherapy were selected, and 45 normal cervical tissues were included as a control. Immunohistochemistry was used to detect the level of P-glycoprotein (P-gp) in cervical cancer, and to make an analysis compared with normal tissues. The methylation status of the MDR1 gene promoter region 16 CpG units was analyzed by using kilobase-specific cracking and matrix-assisted laser desorption ionization time of flight mass spectrometry.
Results: The results indicated that the positive expression rates of P-gp were 0% (0/45) in normal cervical tissue, and 61.19% (41/67) and 77.61% (52/67) before and after interventional embolism chemotherapy in cervical cancer tissues, respectively. There were significant differences compared with normal cervical tissues (χ2=4.2523, 0.0392). The positive expression rate of P-gp before chemotherapy was negatively correlated with efficacy of chemotherapy (r=-0.340, P=0.005). Methylation rate of 13 CpG units in normal tissues was significantly greater than cervical tissues (P<0.05). In cervical cancer tissue, methylation rate of six CpG units before interventional embolism chemotherapy was higher than after chemotherapy, but that of one CpG unit was lower than after chemotherapy (P<0.05). The methylation rate of one CpG unit with effective chemotherapy before chemotherapy was significantly higher than ineffective chemotherapy (P<0.05), and the other CpG units were similar (P>0.05).
Conclusion: P-gp expression level coded by MDR1, methylation status of partial MDR1 gene promoter regions CpG island, is closely related to the efficacy of interventional embolism chemotherapy for cervical cancer before the operation.

Keywords: cervical cancer, interventional embolism chemotherapy, MDR1 gene, methylation, immunohistochemistry

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