Back to Browse Journals » International Journal of Nanomedicine » Volume 6

Ethylenediamine functionalized-single-walled nanotube (f-SWNT)-assisted in vitro delivery of the oncogene suppressor p53 gene to breast cancer MCF-7 cells

Authors Karmakar A, Bratton SM, Dervishi E, Ghosh A, Mahmood M, Xu Y, Saeed L, Mustafa T, Casciano D, Radominska-Pandya A, Biris AS

Published 18 May 2011 Volume 2011:6 Pages 1045—1055

DOI https://doi.org/10.2147/IJN.S17684

Review by Single-blind

Peer reviewer comments 2

Alokita Karmakar2, Stacie M Bratton1, Enkeleda Dervishi2, Anindya Ghosh3, Meena Mahmood2, Yang Xu2, Lamya Mohammed Saeed2, Thikra Mustafa2, Dan Casciano2, Anna Radominska-Pandya1, Alexandru S Biris2
1
Biochemistry Department, University of Arkansas for Medical Sciences; 2Nanotechnology Center, Applied Science Department; 3Department of Chemistry, University of Arkansas, Little Rock, AR, USA

Abstract: A gene delivery concept based on ethylenediamine-functionalized single-walled carbon nanotubes (f-SWCNTs) using the oncogene suppressor p53 gene as a model gene was successfully tested in vitro in MCF-7 breast cancer cells. The f-SWCNTs-p53 complexes were introduced into the cell medium at a concentration of 20 µg mL-1 and cells were exposed for 24, 48, and 72 hours. Standard ethidium bromide and acridine orange assays were used to detect apoptotic cells and indicated that a significantly larger percentage of the cells (approx 40%) were dead after 72 hours of exposure to f-SWCNTs-p53 as compared to the control cells, which were exposed to only p53 or f-SWCNTs, respectively. To further support the uptake and expression of the genes within the cells, green fluorescent protein-tagged p53, attached to the f-SWCNTs was added to the medium and the complex was observed to be strongly expressed in the cells. Moreover, caspase 3 activity was found to be highly enhanced in cells incubated with the f-SWCNTs-p53 complex, indicating strongly induced apoptosis. This system could be the foundation for novel gene delivery platforms based on the unique structural and morphological properties of multi-functional nanomaterials.

Keywords: carbon nanotubes, gene delivery, cancer cells, p53 oncogene suppressor

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Other articles by this author:

Direct electrochemical oxidation of S-captopril using gold electrodes modified with graphene-AuAg nanocomposites

Pogacean F, Biris AR, Coros M, Lazar MD, Watanabe F, Kannarpady GK, Al Said SA Farha, Biris AS, Pruneanu S

International Journal of Nanomedicine 2014, 9:1111-1125

Published Date: 25 February 2014

Few-layer graphene sheets with embedded gold nanoparticles for electrochemical analysis of adenine

Biris AR, Pruneanu S, Pogacean F, Lazar MD, Borodi G, Ardelean S, Dervishi E, Watanabe F, Biris AS

International Journal of Nanomedicine 2013, 8:1429-1438

Published Date: 12 April 2013

Readers of this article also read:

Fabrication and in vitro release behavior of a novel antibacterial coating containing halogenated furanone-loaded poly(L-lactic acid) nanoparticles on microarc-oxidized titanium

Cheng YC, Wu J, Gao B, Zhao XH, Yao JY, Mei SL, Zhang L, Ren HF

International Journal of Nanomedicine 2012, 7:5641-5652

Published Date: 7 November 2012

Supramolecular micellar nanoaggregates based on a novel chitosan/vitamin E succinate copolymer for paclitaxel selective delivery

Lian H, Sun J, Yu YP, Liu YH, Cao W, Wang YJ, Sun YH, Wang SL, He ZG

International Journal of Nanomedicine 2011, 6:3323-3334

Published Date: 12 December 2011

Effect of dispersants of multi-walled carbon nanotubes on cellular uptake and biological responses

Haniu H, Saito N, Matsuda Y, Kim YA, Park KC, Tsukahara T, Usui Y, Aoki K, Shimizu M, Ogihara N, Hara K, Takanashi S, Okamoto M, Ishigaki N, Nakamura K, Kato H

International Journal of Nanomedicine 2011, 6:3295-3307

Published Date: 9 December 2011

Subtle cytotoxicity and genotoxicity differences in superparamagnetic iron oxide nanoparticles coated with various functional groups

Hong SC, Lee JH, Lee J, Kim HY, Park JY, Cho J, Lee J, Han DW

International Journal of Nanomedicine 2011, 6:3219-3231

Published Date: 7 December 2011

RGD-tagged helical rosette nanotubes aggravate acute lipopolysaccharide-induced lung inflammation

Suri SS, Mills S, Aulakh GK, Rakotondradany F, Fenniri H, Singh B

International Journal of Nanomedicine 2011, 6:3113-3123

Published Date: 2 December 2011

A new era of cancer treatment: carbon nanotubes as drug delivery tools

Madani SY, Naderi N, Dissanayake O, Tan A, Seifalian AM

International Journal of Nanomedicine 2011, 6:2963-2979

Published Date: 22 November 2011

Functionalized carbon nanomaterials: exploring the interactions with Caco-2 cells for potential oral drug delivery

Coyuco JC, Liu Y, Tan BJ, Chiu GNC

International Journal of Nanomedicine 2011, 6:2253-2263

Published Date: 10 October 2011

Entrapment in phospholipid vesicles quenches photoactivity of quantum dots

Generalov R, Kavaliauskiene S, Westrøm S, Chen W, Kristensen S, Juzenas P

International Journal of Nanomedicine 2011, 6:1875-1888

Published Date: 7 September 2011

In-vivo imaging of oral squamous cell carcinoma by EGFR monoclonal antibody conjugated near-infrared quantum dots in mice

Yang K, Zhang FJ, Tang H, Zhao C, Cao YA, Lv XQ, Chen D, Li YD

International Journal of Nanomedicine 2011, 6:1739-1745

Published Date: 19 August 2011

Nanotoxicology and nanoparticle safety in biomedical designs

Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, Aminifard S, Zafari M, Akbari HR, Rad HG

International Journal of Nanomedicine 2011, 6:1117-1127

Published Date: 31 May 2011