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Endothelial vascular smooth muscle cell coculture assay for high throughput screening assays to identify antiangiogenic and other therapeutic molecules

Authors George A Truskey

Published 11 October 2010 Volume 2010:1 Pages 171—181

DOI https://doi.org/10.2147/IJHTS.S13459

Review by Single-blind

Peer reviewer comments 2

George A Truskey
Department of Biomedical Engineering, Duke University, Durham, NC, USA

Abstract: Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of coculture systems of endothelial cells and vascular smooth muscle cells can be extended to high-throughput systems for the identification of compounds for angiogenesis, vascular repair, and hypertension. In this review, the various coculture systems are reviewed, and biologic interactions between endothelial cells and vascular smooth muscle cells are discussed. Key considerations in the design of high-throughput systems are presented, and selected examples are discussed.

Keywords: endothelium, vascular smooth muscle cells, angiogenesis, microfluidics

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