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Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats

Authors Dota A, Sakamoto A, Nagano T, Murakami T, Matsugi T

Received 17 December 2019

Accepted for publication 27 February 2020

Published 1 April 2020 Volume 2020:14 Pages 1019—1024


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Dr Scott Fraser

Atsuyoshi Dota, Asuka Sakamoto, Takashi Nagano, Tadahiro Murakami, Takeshi Matsugi

Nara Research & Development Center, Santen Pharmaceutical Co., Ltd, Ikoma-shi, Nara, Japan

Correspondence: Atsuyoshi Dota
Nara Research & Development Center, Santen Pharmaceutical Co., Ltd., 8916-16 Ikoma-shi, Nara 630-0101, Japan
Tel +81 743 79 4525
Fax +81 743 79 4591

Purpose: To examine the effect of 3% diquafosol ophthalmic solution (DQS) on ocular surface disorders in diabetic model rats maintained in a continuous airflow condition.
Methods: Goto–Kakizaki (GK) rats, a spontaneous model of type 2 diabetes, were exposed to constant airflow for 8 weeks. After the establishment of the animal model in this environment, DQS or saline was instilled six times a day into GK rat eyes for 6 weeks. Schirmer’s test was performed before and after 6-week instillations. Corneal fluorescein staining was scored at 2-, 4-, and 6-week instillations. Touch thresholds for the cornea were also determined using a Cochet–Bonnet esthesiometer before and after 6-week instillations.
Results: The mean Schirmer’s test score after instillation of DQS was twice higher than that recorded for saline alone. DQS significantly decreased corneal staining scores at 4- and 6-week instillations. No changes in touch thresholds were observed before and after 6-week instillations.
Conclusion: These results suggest that DQS improves corneal epithelial damage by stimulating tear secretion without influencing corneal sensation in diabetic keratopathy. Thus, DQS may have potential for treatment of diabetic patients with dry eye.

Keywords: diquafosol ophthalmic solution, corneal epithelial damage, tear volume, diabetic keratopathy, Goto–Kakizaki rat

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