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Cryopreservation in mammalian conservation biology: current applications and potential utility

Authors Silva AR, Lima G, Peixoto G, Souza AL

Received 30 October 2014

Accepted for publication 30 January 2015

Published 20 March 2015 Volume 2015:4 Pages 1—8

DOI https://doi.org/10.2147/RRBS.S54294

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 6

Editor who approved publication: Professor Neil Cumberlidge


Alexandre Rodrigues Silva, Gabriela Liberalino Lima, Gislayne Christianne Xavier Peixoto, Ana Liza Paz Souza

Laboratório de Conservação de Germoplasma Animal, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil


Abstract: A rapid and continuous decline in wild mammalian species populations has been documented in recent decades. Although in situ conservation strategies such as habitat preservation are usually the best way to preserve biodiversity, other rescue strategies such as germplasm cryopreservation are sometimes necessary. Germplasm cryopreservation involves the freezing of gametes, embryos, gonadal tissues, or somatic tissues of species threatened with extinction. There is substantial diversity in the cryobiological requirements among cell types and tissues of each species. Research has focused on adapting techniques developed for the conservation of the genetic material of domestic animals for use with wild species about which relatively little is known. This review describes and discusses the current and potential use of cryobanking for the preservation of cells and tissues of threatened species.

Keywords: cryobanking, gametes, somatic cells, germplasm preservation

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