Back to Journals » Clinical and Experimental Gastroenterology » Volume 13

Circulating α4β7+ Memory T Cells in Pediatric IBD Patients Express a Polyclonal T Cell Receptor Repertoire

Authors Gamliel A, Werner L, Pinsker M, Salamon N, Weiss B, Shouval DS

Received 10 July 2020

Accepted for publication 21 August 2020

Published 2 October 2020 Volume 2020:13 Pages 439—447


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Prof. Dr. Wing-Kin Syn

Adir Gamliel,1,2 Lael Werner,1,2 Marina Pinsker,1,3 Naomi Salamon,1 Batia Weiss,1,2 Dror S Shouval1,2

1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children’s Hospital, Sheba Medical Center, Ramat Gan, Israel; 2Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; 3Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat Gan, Israel

Correspondence: Dror S Shouval
Pediatric Gastroenterology Unit, Edmond and Lily Safra Children’s Hospital, Sheba Medical Center, Ramat Gan, Israel
Tel +972-3-5305006

Background: The integrin α 4β 7 is highly expressed on activated T cells and is thought to direct homing of lymphocytes to the intestine. Since ulcerative colitis (UC) and Crohn’s disease (CD) are characterized by mucosal oligoclonal T cells’ expansion, we aimed to assess whether similar repertoire features are identified in circulating gut-specific memory T cells.
Methods: Memory CD3+ T cells were isolated from blood samples of control subjects and patients with active UC or CD and then FACS-sorted into α 4β 7+ and α 4β 7 populations. DNA was extracted from each subset and subjected to next-generation sequencing of the TCRβ. Different repertoire characteristics were compared between α 4β 7+ and α 4β 7 subsets for each subject, and between groups.
Results: The percentages of memory T cells and α 4β 7+ cells were comparable between groups. α 4β 7+ memory T cells displayed a polyclonal distribution, in control subjects and in UC or CD patients, with similar indices of diversity. Strikingly, the clonal overlap between α 4β 7+ and α 4β 7 T cells for each subject in all three groups was high, ranging between 20%– 50%. We were unable to identify shared T cell clones that were specific to one of the groups.
Conclusion: α 4β 7+ memory T cells exhibited a polyclonal repertoire in both control subjects and patients with active inflammatory bowel disease, with high rates of overlap with α 4β 7 memory T cells. Our study, along with additional recent reports, may suggest that the suppression of intestinal inflammation by vedolizumab is independent of the drug’s effect on T cell migration to the gut.

Keywords: IBD, TCR, integrin, α 4β 7, T cells, immune repertoire

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]