Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro, Brazil
Authors Fabrício-Silva GM, Poschetzky B, Perez R, Carneiro dos Santos R, Cavalini L, Porto LC
Received 28 May 2015
Accepted for publication 29 July 2015
Published 2 November 2015 Volume 2015:7 Pages 71—79
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 3
Editor who approved publication: Dr Gerry Lake-Bakaar
Gustavo Milson Fabrício-Silva,1 Bruno Silva Poschetzky,2 Renata de Mello Perez,2 Ronaldo Carneiro dos Santos,2 Luciana Tricai Cavalini,3 Luís Cristóvão Porto1
1Histocompatibility and Cryopreservation Laboratory, 2Gastroenterology Service, Internal Medicine Department, 3Information Technology and Healthy Education Department, Rio de Janeiro State University, Rio de Janeiro, Brazil
Background: The host immune response is an important indicator of the outcome of hepatitis C virus (HCV) infection and disease progression. The aim of this study was to explore cytokine gene polymorphisms as a candidate for susceptibility to persistent HCV infection or HCV spontaneous clearance in a population from Rio de Janeiro, Brazil.
Methods: Genetic polymorphisms in the cytokines, tumor necrosis factor-alpha (–308), transforming growth factor-beta 1 (codons 10 and 25), interleukin-10 (IL-10; –1082 and –592), IL-6 (–174), and interferon-gamma (+874) were analyzed by polymerase chain reaction sequence-specific primers in 245 patients with chronic hepatitis C (CHC), 41 spontaneous recovery (SR) patients, and 189 healthy volunteers. Further, polymorphisms in IL-28B (rs12979860, rs12980275, and rs8099917) were assessed by real-time polymerase chain reaction in all groups.
Results: The IL-28B rs12979860 CC and rs12980275 AA genotypes were significantly associated with SR of HCV infection and response to therapy, whereas the C allele of IL-6 (–174) was associated with protection to CHC. A multivariate analysis showed that the male sex and IL-28B rs12979860 CT and TT and transforming growth factor-beta 1 (codon 10) TC genotypes were factors associated with CHC. Additionally, IL-6 (–174) C allele was increased in SR patients compared with patients with CHC.
Conclusion: IL-28B polymorphisms are associated with spontaneous clearance of HCV and response to therapy in a Brazilian population. Also, IL-6 (–174) C allele is involved in SR and decreased inflammation scores.
Keywords: interleukin-28, interleukin-6, SNP, inflammation, HCV clearance
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