Back to Journals » International Journal of Nanomedicine » Volume 13

Amino acid composition of nanofibrillar self-assembling peptide hydrogels affects responses of periodontal tissue cells in vitro

Authors Koch F, Wolff A, Mathes S, Pieles U, Saxer SS, Kreikemeyer B, Peters K

Received 10 May 2018

Accepted for publication 17 July 2018

Published 23 October 2018 Volume 2018:13 Pages 6717—6733


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Thomas Webster

Video abstract presented by Franziska Koch

Views: 246

Franziska Koch,1–3 Anne Wolff,2 Stephanie Mathes,4 Uwe Pieles,1 Sina S Saxer,1 Bernd Kreikemeyer,3 Kirsten Peters2

1Institute for Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Muttenz, Switzerland; 2Department of Cell Biology, University Medicine Rostock, Rostock, Germany; 3Institute of Medical Microbiology, Virology and Hygiene, University Medicine Rostock, Rostock, Germany; 4Department for Chemistry and Biotechnology, Tissue Engineering, Zurich University of Applied Sciences, Wädenswil, Switzerland

Background: The regeneration of tissue defects at the interface between soft and hard tissue, eg, in the periodontium, poses a challenge due to the divergent tissue requirements. A class of biomaterials that may support the regeneration at the soft-to-hard tissue interface are self-assembling peptides (SAPs), as their physicochemical and mechanical properties can be rationally designed to meet tissue requirements.
Materials and methods: In this work, we investigated the effect of two single-component and two complementary β-sheet forming SAP systems on their hydrogel properties such as nanofibrillar architecture, surface charge, and protein adsorption as well as their influence on cell adhesion, morphology, growth, and differentiation.
Results: We showed that these four 11-amino acid SAP (P11-SAP) hydrogels possessed physicochemical characteristics dependent on their amino acid composition that allowed variabilities in nanofibrillar network architecture, surface charge, and protein adsorption (eg, the single-component systems demonstrated an ~30% higher porosity and an almost 2-fold higher protein adsorption compared with the complementary systems). Cytocompatibility studies revealed similar results for cells cultured on the four P11-SAP hydrogels compared with cells on standard cell culture surfaces. The single-component P11-SAP systems showed a 1.7-fold increase in cell adhesion and cellular growth compared with the complementary P11-SAP systems. Moreover, significantly enhanced osteogenic differentiation of human calvarial osteoblasts was detected for the single-component P11-SAP system hydrogels compared with standard cell cultures.
Conclusion: Thus, single-component system P11-SAP hydrogels can be assessed as suitable scaffolds for periodontal regeneration therapy, as they provide adjustable, extracellular matrix-mimetic nanofibrillar architecture and favorable cellular interaction with periodontal cells.

Keywords: self-assembling peptides, SAPs, P11-SAP hydrogels, surface charge, protein adsorption, cell proliferation, osteogenic differentiation, periodontal tissue regeneration

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]