Daunorubicin-loaded magnetic nanoparticles of Fe₃O₄ overcome multidrug resistance and induce apoptosis of K562-n/VCR cells in vivo

Original Research

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Authors: Bao-an Chen, Bin-bin Lai, Jian Cheng, et al

Published Date September 2009 Volume 2009:4 Pages 201 - 208

DOI: http://dx.doi.org/10.2147/IJN.S7287

Bao-an Chen¹, Bin-bin Lai¹, Jian Cheng¹, Guo-hua Xia¹, Feng Gao¹, Wen-lin Xu², Jia-hua Ding¹, Chong Gao¹, Xin-chen Sun³, Cui-rong Xu¹, Wen-ji Chen¹, Ning-na Chen¹, Li-jie Liu⁴, Xiao-mao Li⁵, Xue-mei Wang⁶

¹Department of Hematology, ³Department of Oncology, the Affiliated Zhongda Hospital, Clinical Medical School, Southeast University, Nanjing, People’s Republic of China; ²Department of Hematology, the Affiliated People’s Hospital, Jiangsu University, Zhenjiang, People’s Republic of China; ⁴Institution of Physiology, ⁶State Key Lab of Bioelectronics (Chien-shiung Wu Laboratory), Southeast University, Nanjing, People’s Republic of China; ⁵Department of Physics, University of Saarland, Saarbruechen, Germany

Abstract: Multidrug resistance (MDR) is a major obstacle to cancer chemotherapy. We evaluated the effect of daunorubicin (DNR)-loaded magnetic nanoparticles of Fe₃O₄ (MNPs-Fe₃O₄) on K562-n/VCR cells in vivo. K562-n and its MDR counterpart K562-n/VCR cell were inoculated into nude mice subcutaneously. The mice were randomly divided into four groups: group A received normal saline, group B received DNR, group C received MNPs-Fe₃O₄, and group D received DNR-loaded MNPs-Fe₃O₄. For K562-n/VCR tumor, the weight was markedly lower in group D than that in groups A, B, and C. The transcriptions of Mdr-1 and Bcl-2 gene were significantly lower in group D than those in groups A, B, and C. The expression of Bcl-2 was lower in group D than those in groups A, B, and C, but there was no difference in the expression of P-glycoprotein. The transcriptions and expressions of Bax and caspase-3 in group D were increased significantly when compared with groups A, B, and C. In conclusion, DNR-loaded MNPs-Fe₃O₄ can overcome MDR in vivo.

Keywords: multidrug-resistance reversal, leukemia, magnetic nanoparticles of Fe₃O₄, in vivo

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