Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 2

Quantum dot-doped silica nanoparticles as probes for targeting of T-lymphocytes

Authors Massimo Bottini, Federica D’Annibale, Andrea Magrini, Fabio Cerignoli, Yutaka Arimura, Marcia I Dawson, Enrico Bergamaschi, Nicola Rosato, Antonio Bergamaschi, Tomas Mustelin

Published Date August 2007 Volume 2007:2(2) Pages 227—233

DOI

Published 16 August 2007

Massimo Bottini1,2,4, Federica D’Annibale2, Andrea Magrini2, Fabio Cerignoli1, Yutaka Arimura1, Marcia I Dawson1, Enrico Bergamaschi5, Nicola Rosato3, Antonio Bergamaschi1, Tomas Mustelin1

1Burnham Institute for Medical Research, La Jolla, CA, USA; 2NAST and Department of Environmental, Occupational and Social Medicine, University of Rome Tor Vergata, Rome, Italy; 3NAST and Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Rome, Italy; 4INFN, Laboratori Nazionali di Frascati, Frascati, Italy; 5Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Parma, Italy

Abstract: To enhance diagnostic or therapeutic efficacy, novel nanomaterials must be engineered to function in biologically relevant environments, be visible by conventional fluorescent microscopy, and have multivalent loading capacity for easy detection or effective drug delivery. Here we report the fabrication of silica nanoparticles doped with quantum dots and superficially functionalized with amino and phosphonate groups. The amino groups were acylated with a water-soluble biotin-labeling reagent. The biotinylated nanoparticles were subsequently decorated with neutravidin by exploiting the strong affinity between neutravidin and biotin. The resultant neutravidin-decorated fluorescent silica nanoparticles stably dispersed under physiological conditions, were visible by conventional optical and confocal fluorescent microscopy, and could be further functionalized with macromolecules, nucleic acids, and polymers. We also coated the surface of the nanoparticles with biotinylated mouse anti-human CD3 (αCD3). The resultant fluorescent nanoassembly was taken up by Jurkat T cells through receptor-mediated endocytosis and was partially released to lysosomes. Thus, quantum dotdoped silica nanoparticles decorated with neutravidin represent a potentially excellent scaffold for constructing specific intracellular nanoprobes and transporters.

Keywords: silica nanoparticles, neutravidin, surface functionalization, endocytosis, intracellular nanoprobe

Download Article [PDF] 

Readers of this article also read:

Recurrent nontuberculous mycobacterial endophthalmitis: a diagnostic conundrum

Venkateswaran N, Yeaney G, Chung M, Hindman HB

Clinical Ophthalmology 2014, 8:837-842

Published Date: 2 May 2014

Bilateral visual outcomes and service utilization of patients treated for 3 years with ranibizumab for neovascular age-related macular degeneration

Chavan R, Panneerselvam S, Adhana P, Narendran N, Yang Y

Clinical Ophthalmology 2014, 8:717-723

Published Date: 8 April 2014

Perioperative hyperglycemia is associated with postoperative neurocognitive disorders after cardiac surgery

Zhang X, Yan X, Gorman J, Hoffman SN, Zhang L, Boscarino JA

Neuropsychiatric Disease and Treatment 2014, 10:361-370

Published Date: 19 February 2014

Synthesis, characterization, and evaluation of paclitaxel loaded in six-arm star-shaped poly(lactic-co-glycolic acid)

Chen Y, Yang Z, Liu C, Wang C, Zhao S, Yang J, Sun H, Zhang Z, Kong D, Song C

International Journal of Nanomedicine 2013, 8:4315-4326

Published Date: 7 November 2013

Polyacrylonitrile nanofibers coated with silver nanoparticles using a modified coaxial electrospinning process

Yu DG, Zhou J, Chatterton NP, Li Y, Huang J, Wang X

International Journal of Nanomedicine 2012, 7:5725-5732

Published Date: 12 November 2012

Actively-targeted LTVSPWY peptide-modified magnetic nanoparticles for tumor imaging

Jie L-Y, Cai L-L, Wang L-J, Ying X-Y, Yu R-S, Zhang M-M,Du Y-Z

International Journal of Nanomedicine 2012, 7:3981-3989

Published Date: 24 July 2012

Lysine-functionalized nanodiamonds: synthesis, physiochemical characterization, and nucleic acid binding studies

Kaur R, Chitanda JM, Michel D, Maley J, Borondics F, Yang P, Verrall RE, Badea I

International Journal of Nanomedicine 2012, 7:3851-3866

Published Date: 19 July 2012

Depressive mixed state: Evidence for a new form of depressive state in type I and II bipolar patients

Katia M’Bailara, Donatienne Van den Bulke, Nicolas Demazeau, Jacques Demotes-Mainard, Chantal Henry

Neuropsychiatric Disease and Treatment 2007, 3:899-902

Published Date: 21 January 2007