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Insulin administration: present strategies and future directions for a noninvasive (possibly more physiological) delivery

Authors Matteucci E, Giampietro O, Covolan V, Giustarini D, Fanti P, Rossi R

Received 15 December 2014

Accepted for publication 12 February 2015

Published 17 June 2015 Volume 2015:9 Pages 3109—3118

DOI https://doi.org/10.2147/DDDT.S79322

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 3

Editor who approved publication: Professor Shu-Feng Zhou

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Elena Matteucci,1 Ottavio Giampietro,1 Vera Covolan,2 Daniela Giustarini,3 Paolo Fanti,4 Ranieri Rossi3

1Department of Clinical and Experimental Medicine, 2Department of Chemistry and Industrial Chemistry, University of Pisa, 3Department of Life Sciences, Laboratory of Pharmacology and Toxicology, University of Siena, Siena, Italy; 4Division of Nephrology, University of Texas Health Science Center San Antonio, South Texas Veteran Health Care System, San Antonio, Texas, USA

Abstract: Insulin is a life-saving medication for people with type 1 diabetes, but traditional insulin replacement therapy is based on multiple daily subcutaneous injections or continuous subcutaneous pump-regulated infusion. Nonphysiologic delivery of subcutaneous insulin implies a rapid and sustained increase in systemic insulin levels due to the loss of concentration gradient between portal and systemic circulations. In fact, the liver degrades about half of the endogenous insulin secreted by the pancreas into the venous portal system. The reverse insulin distribution has short- and long-term effects on glucose metabolism. Thus, researchers have explored less-invasive administration routes based on innovative pharmaceutical formulations, which preserve hormone stability and ensure the therapeutic effectiveness. This review examines some of the recent proposals from clinical and material chemistry point of view, giving particular attention to patients’ (and diabetologists’) ideal requirements that organic chemistry could meet.

Keywords: type 1 diabetes mellitus, drug formulations, drug administration routes, insulin, portal system, nanoparticles, biodegradable polymers

Introduction

Patients with type 1 diabetes rely on the exogenous delivery of insulin because of impaired insulin secretion by the beta cells of the endocrine pancreas. In current practice, glycemic control is achieved through the use of basal and prandial insulin (multiple daily injections [MDIs]) or by continuous subcutaneous insulin infusion (CSII) using an external pump. Self-injecting insulin is unpleasant for patients, and the need for frequent self-monitoring of blood glucose (SMBG) by finger sticks presupposes motivation and implies pain, costs, technical skills, and psychological burden. Recent systematic reviews examined the comparative effectiveness of methods for insulin delivery and glucose monitoring and identified research priorities.1,2 Intensive insulin therapy delivered either by CSII or MDI was equally effective in lowering glycated hemoglobin (HbA1c) levels and resulted in similar rates of hypoglycemia in both adolescents and adults with type 1 diabetes.2 Real-time continuous glucose monitoring (rt-CGM) was superior to SMBG in lowering HbA1c, without increasing the risk of hypoglycemia.2 Although CSII and MDI without rt-CGM had similar effects on HbA1c, the addition of rt-CGM to CSII was associated with lower HbA1c levels than MDI/SMBG.2 For type 1 diabetes, expert stakeholders ranked adolescents as the highest priority age group and artificial pancreas as the highest priority for future research. For glucose monitoring methods, all stakeholders prioritized rt-CGM for any patient with type 1 diabetes.2 In patients with type 2 diabetes, CSII therapy was not superior to MDI.3 Closed-loop insulin delivery, so-called artificial pancreas, combines continuous glucose sensor with insulin infusion pump using validated mathematical algorithms to drive CSII. These systems, which include the recent dual-hormone closed-loop delivery devices, could improve glycemic control and reduce the risk of hypoglycemia. One of the major disadvantages of insulin pump therapy is cost, which is higher than MDI (http://www.idf.org/debate-insulin-pump-therapy-matter-choice).

Whatever the regimen, the common drawback of both CSII and MDI remains that subcutaneous insulin is absorbed nonphysiologically into the systemic circulation with consequent peripheral hyperinsulinemia, weight gain, and risk of hypoglycemia. This review focuses on noninvasive approaches for insulin delivery with particular attention to systems potentially able to reproduce physiological insulin secretion.

Physiology of insulin secretion

Circulating, monomeric insulin is composed of two polypeptide chains (the A and B chains consisting of 21 and 30 amino acids, respectively) and two disulfide bridges, which create the quaternary assembly of the molecule (http://www.betacell.org/content/articleview/article_id/8/) (Accessed on February 4, 2015).

Human insulin is synthesized as preproinsulin (110 amino acids) in the rough endoplasmic reticulum. Following removal of the first 24 amino acids (signal peptide) and packaging in the Golgi apparatus, insulin is stored as proinsulin in the immature secretory granules. The conversion of proinsulin into active insulin and C-peptide is catalyzed by the proteolytic activity of proinsulin convertase 1, proinsulin convertase 2, and carboxypeptidase H (Figure 1). Insulin is secreted by the beta cells: every beta cell contains 10,000–13,000 secretory granules and a single insulin granule contains ~106 molecules of insulin.4

Figure 1 Schematic representation of the amino acid sequence of the human preproinsulin (A) and the conversion of proinsulin into biologically active insulin and C-peptide (B).

The beta cell is electrically excitable: calcium-dependent exocytosis of the secretory granules is regulated by variations in plasma glucose concentration via changes in beta cell metabolism and closure of KATP channels.4 In isolated islets, insulin secretion is biphasic with a first-phase component, which lasts a few minutes, and a slowly developing but sustained second-phase component. The first-phase secretion involves the docked granules (or possibly the so-called restless newcomers, ie, granules that are recruited by stimulation and immediately released), while the second-phase secretion involves the time- and ATP-dependent supply of new granules.4 The secretory rate of beta cells in intact islets has been estimated ~15 granules per beta cell per second.4

Assessing insulin secretion in vivo is quite more complex: 1) insulin is secreted in high-frequency pulses (recurring every 5–15 minutes) superimposed on slower, ultradian oscillations (every 80–120 minutes). Glucose administration mainly amplifies secretory burst amplitude/mass; 2) insulin pulses are secreted in the portal vein and undergo ~40%–80% first-pass hepatic extraction with consequent waveform damping in the systemic circulation. The amplitude of insulin pulses is the principal determinant of hepatic insulin clearance; 3) plasma insulin levels in the peripheral circulation reflect hormone secretion, distribution, and degradation; 4) C-peptide is secreted in equimolar amounts with insulin but is more slowly catabolized than insulin. Unlike insulin, C-peptide is not extracted by the liver, thus C-peptide secretion rate can be estimated from plasma C-peptide levels; 5) proinsulin and insulin are not released equimolarly, and proinsulin clearance is lower than that of insulin. Circulating proinsulin accounts for ~10%–20% of normal fasting insulin, but it may be disproportionately increased in type 2 diabetes; however, highly specific immunoassay methods are required to differentiate between intact proinsulin and the specific and unspecific proinsulin derivatives.58

In the basal state, the range of the insulin concentration is ~100–1,000 pmol/L in the portal circulation vs 10–30 pmol/L in the systemic circulation. According to Meier et al the greater extraction of insulin within insulin secretory bursts is predicted by insulin receptor-binding kinetics; therefore, the pulse mass of insulin release dictates not only hepatic insulin delivery but also its fractional hepatic extraction (and in turn the delivery of insulin into the systemic circulation).8

Insulin inhibits directly hepatic glucose production through inhibition of gluconeogenesis and glycogenolysis; indirect effects include inhibition of glucagon secretion, lipolysis in fat, and proteolysis in muscle. During a pancreatic clamp in the overnight-fasted, conscious dog, insulin infusion was switched from the hepatic portal vein to a peripheral vein; as a result, Edgerton et al obtained a doubling of the arterial insulin level and a 50% decrease in the insulin level within the hepatic sinusoids. The arterial plasma free fatty acids level and net hepatic free fatty acid uptake decreased by 40%–50%; hepatic glucose output increased more than twofold and remained elevated compared with that in the control group.9 Insulin’s effects include reduction of glucagon secretion. Glucagon is a counterregulatory hormone that promotes glycogenolysis and inhibits glycogen synthesis in the liver, increases gluconeogenesis, and decreases glycolysis. The absolute levels or rather the ratios of glucagon to insulin are key regulators of glucose homeostasis and have been found to be elevated in patients with diabetes.10

Exogenously administered insulin and alternative noninvasive routes of delivery

Exogenously administered insulin, usually by subcutaneous injection, is unable to mimic endogenously secreted insulin. In normal physiology, the liver is exposed to insulin concentrations two- to fourfold higher than those observed in the peripheral circulation. Subcutaneously injected insulin is unable to approach this portal-systemic insulin concentration gradient and results in impaired hepatic glucose suppression.10 Relative insulin deficiency in the portal circulation of liver compromises 1) the regulation of the rate of hepatic glycogenolysis and gluconeogenesis during fasting and 2) paracrine suppression of glucagon secretion in the fed state. As a consequence, in the postprandial state, there is an inappropriate elevation of glucagon and depletion of glycogen stores.10 Moreover, increasing insulin doses leads to peripheral hyperinsulinemia, which predisposes to hypoglycemia and weight gain.10

After an overnight fast, the normal arterial plasma insulin level ranges from 5 μU/mL to 15 μU/mL, whereas the level of insulin in the portal vein is approximately threefold greater. The plasma insulin concentration in the liver sinusoids, which contain mixed arterial (20%) and portal (80%) blood, ranges from ∼15 μU/mL to 40 μU/mL.11 If fasting insulin secretion was totally absent, one could reproduce the peripheral fasting plasma insulin concentrations observed in nondiabetic subjects by infusing 0.35–0.56 U/kg per day intraportally, whereas 0.08–0.11 U/kg per day by peripheral insulin administration.12 It is noteworthy that the dose–response curve for the effect of insulin on fasting hepatic glucose output is shifted to the left relative to the dose–response curve of peripheral insulin action. A doubling of insulin secretion inhibits hepatic glucose output by ∼80%, while it increases glucose utilization by only 20%; the effect of insulin on glucose production is complete when insulin secretion increases threefold, while the effect of insulin on glucose utilization does not saturate even with the highest possible physiologic insulin levels.11

Different routes of insulin administration (oral, pulmonary, transdermal, intranasal, ocular, vaginal, and rectal) have been explored.13,14 However, no alternative route of systemic insulin administration can reproduce a positive portal-systemic blood insulin gradient (Figure 2). Unlike transdermal or pulmonary drug delivery routes, substances that are absorbed in the gastrointestinal tract are drained to the liver via the portal vein. The hepatic portal vein was formed by the junction of the superior mesenteric and splenic veins, with the inferior mesenteric vein joining at or near the angle of union; it drains venous blood from the gastrointestinal tract, spleen, pancreas, and gallbladder. Hence, oral insulin absorption into the portal vein could generate a high portal-systemic gradient, mimicking the endogenous secretion of insulin.

Figure 2 Portal-systemic blood insulin gradient and alternative routes of insulin administration.

Transdermal delivery of insulin

The skin is composed of three layers: the stratum corneum, epidermis, and dermis. It not only provides both a physical and a chemical barrier against foreign invaders but also functions as an active immune organ. The stratum corneum consists of nonnucleated protein-enriched corneocytes (anchored together by means of corneodesmosomes) and lipid-enriched intercellular domains. The epidermis is a multilayered, epithelial tissue divided into the basal cell layer, the spinous cell layer, and the granular cell layer. The papillary dermis and reticular dermis contain collagen and elastic fibers, proteoglycans, and glycoproteins; the dermal vasculature, lymphatics, nerves, sweat glands, and hair roots are embedded within the fibrous tissue. The skin immune system includes keratinocytes and Langerhans cells in the epidermis; fibroblasts, dendritic cells, and mast cells in the dermis; and T- and B-lymphocytes in the skin-draining lymph nodes. Easy access to this skin immune system has been considered an attractive site for vaccination.15

The transdermal permeation of insulin is now extensively investigated in in vitro and in vivo studies conducted in animal models and human subjects. Several techniques have been explored to reduce the skin barrier properties. Penetration enhancers act by various mechanisms such as: 1) increased drug solubility (chemical enhancers), 2) optimization of the formulation (chemical modification, encapsulation within carrier systems), 3) increased diffusion coefficients (microdermabrasion, laser ablation, chemical and biochemical enhancers, ultrasound, electroporation, microneedles), and 4) provision of additional driving force (ultrasound, iontophoresis, electroporation).16,17 Most approaches to increasing skin permeability seek to breach the stratum corneum barrier without damaging viable epidermis. However, viable epidermis also poses a significant barrier to transdermal diffusion of small hydrophilic molecules and macromolecules even in the absence of stratum corneum.18 The application of the photomechanical waves allowed 6 kDa protein molecules to cross the stratum corneum and to reach the systemic circulation;19 after photomechanical insulin delivery in a streptozotocin-diabetic rat model, blood glucose decreased (80%±3%) and remained below 200 mg/dL for more than 3 hours.19 Skin permeation after laser ablation was proportional to the treated energy: electron micrographs of pig skin treated by erbium:yttrium–aluminum–garnet (Er:YAG) laser showed a disarrangement of the stratum corneum surface, wider intervening spaces between the corneocyte aggregates,20 and some of the corneocytes were disrupted probably as a result of laser-induced photomechanical stress. The authors conclude that major prerequisite of a permeation-enhancing method is that the skin recover its normal barrier properties following removal of the enhancement method, but the stratum corneum and epidermis could completely recover within 3–4 days after laser treatment based on different fluences used.20 Most recent approaches toward transdermal insulin delivery include: 1) the conductive polymer nanotube transdermal patch, specifically designed for hydrophilic drugs and insulin;21 2) the iontophoresis treatment with liposomes encapsulating insulin;22 3) the transferosomal (highly deformable vesicles) drug delivery system (gel) that has demonstrated prolonged hypoglycemic effect in alloxan-induced diabetic rats after transdermal administration;23 4) the dissolving polymer microneedle patches that enable stable encapsulation of insulin and produce a significant hypoglycemic effect in diabetic rats, with a relative pharmacological availability and relative bioavailability of 92.2% and 92.3%, respectively.24 When the conductive polymer nanotube transdermal patch was loaded with insulin and delivered ex vivo at a potential of −1.2 V, the cumulative amount of insulin released through porcine skin over 24 hours was substantially less than control dye molecules.21 In streptozotocin-induced type 1 diabetic rats, blood glucose levels decreased slowly after iontophoretic administration of liposomes encapsulating insulin (0.66 mg/kg =18.3 IU/kg), reducing to ∼20% of basal levels 18 hours after iontophoresis; these lower blood glucose levels were maintained for up to 24 hours after administration.22 Over 24 hours after transdermal application of optimized transferosomal gel (containing 2.24 mg insulin) in alloxan-induced diabetic rats, mean blood glucose level was lower by about 30% as compared to that in untreated rats.23 In diabetic rats, the insulin-loaded microneedles reduced blood glucose levels to ∼40% of basal levels at 120 minutes after administration; the relative pharmacological availability and relative bioavailability of insulin were both ∼92%.24 Although the field has continued to evolve and improve over time, efficacy studies of transdermal insulin delivery to human subjects are preliminary18,25 and there are a number of safety aspects that need to be addressed. Safety issues may be associated with strategies that overcome the stratum corneum barrier properties, including pain, local reactions (irritant or allergic contact dermatitis), and infections.17

Inhaled insulin

The inhalation route of biopharmaceutical drug delivery has the advantage of rapid absorption due to the large surface area (~145 m2) and the close proximity of the air and blood compartments. However, deep lung delivery of insulin is influenced by several factors, first of all aerodynamic particle size, particle speed, and ventilatory parameters.26,27 Biopharmaceuticals with a mass median aerodynamic diameter of 1–3 μm reach the alveolar surfaces where they may undergo different fates: clearance by alveolar macrophages, binding to surfactant, and/or enzymatic degradation; alveolar absorption of proteins is slow when their molecular weight is high. Moreover, patient’s ability to perform an appropriate inspiration maneuver is another determinant for reproducible delivery of drug to the distal part of the lung. The efficiency of delivery for an inhaled drug depends on the fraction of dose delivered from the device, the fraction deposited in the alveolar region, and the bioavailable fraction that is absorbed.27 Inhalers generally belong to four categories: metered dose inhalers, spacers and holding chambers, dry powder inhalers, and nebulizers.28 Inhaled insulin has been extensively studied and first inhalable formulation (Exubera) gained market approval in 2006 but was withdrawn in 2007 due to low cost-effectiveness.29 Other inhaled insulin devices were developed (such as AERx Diabetes Management System, AIR, and Technosphere), which differed in the formulation (liquid or powder) and the delivery device. However, the development of these products was discontinued, except for Technosphere insulin that is delivered via a breath-powered inhaler system and received FDA approval in 2014.30 Inhaled insulin showed low efficiency of delivery (10%–12%), which was further affected by pulmonary diseases and smoking,26,27 and induced higher levels of circulating antibodies than comparator insulins given by subcutaneous routes, especially in patients with type 1 diabetes.31 Moreover, it was associated with an increased incidence of cough and a greater decrease in diffusing capacity of the lung for carbon monoxide than subcutaneous insulin in patients with type 1 diabetes.32 Nevertheless, in a recent study that evaluated pulmonary function changes in diabetes patients receiving inhaled Technosphere insulin, changes in lung function were judged not clinically meaningful since they were small, occurred early after therapy initiation, and remained nonprogressive over 2 years.33

Pharmacokinetics studies showed that the total insulin exposure for inhaled insulin was comparable to that of subcutaneous regular insulin, but the exposure time was shorter; inhaled insulin was found to be as clinically effective as injected short-acting insulin.34,35 In large-scale studies, inhaled insulin was effective, well tolerated and well accepted in patients with type 1 diabetes.3639 However, the limited bioavailability of inhaled insulin, which implies considerably higher costs, together with the uncertainties about the efficacy and safety, induced many sponsors to terminate inhaled insulin programs.26,27,3133,3639

Oral insulin delivery

The oral route is the most preferred form of chronic drug administration. Oral medication for diabetic patients treated throughout their lives represents a crucial demand in order to improve their quality of life and to assure adherence to the treatment regimen. Moreover, oral insulin is delivered directly to the liver via portal circulation and could generate a high portal-systemic gradient replicating the endogenous secretion of insulin.40 Nevertheless, effective oral insulin delivery remains challenging because of poor bioavailability.41,42 In the gastrointestinal tract, the absorption of protein and peptide molecules is hampered by physical and biochemical barriers (epithelium, variable pH, enzymatic proteolysis, efflux pumps, and first-pass elimination by liver); solubility, molecular weight, and partition coefficient are the major physicochemical concerns dictating drug dissolution and permeability through the gastrointestinal barrier.41 The pharmacokinetic and pharmacodynamic properties of oral insulin formulations change as a function of the site (along the gastrointestinal tract) and pathway (cellular or paracellular) of absorption. The fast absorption has been attributed to paracellular pathways, whereas the slow absorption to cell pathways (endocytosis via enterocytes or M-cells).40,4345

The application of nanotechnologies in drug delivery is expected to achieve multiple goals: i) shielding the entrapped drugs from the gastrointestinal environment so that they can reach intact the site of absorption; ii) enhancing drug water solubility; iii) enhancing the intestinal permeability of drugs once carried by nanoparticles (NPs) that are chiefly taken up by M-cells, known for their high transcytotic capacity and low lysosomal hydrolase activity; and iv) reducing dosing frequency because of the controlled and sustained release of the nanoencapsulated drugs, and thus improving treatment adherence.41

To improve oral bioavailability of insulin, different types of delivery systems and functional excipients have been explored: capsules, tablets, microparticles, micelles, liposomes, solid lipid NPs and NPs of biodegradable polymers, hydrogels, film patches, superporous matrices, intestinal patches, charge-coupled micromagnet microparticles, polymeric adhesives, protease inhibitors, insulin aggregation inhibitors, permeation enhancers, etc.40,41,46 Depending on their constitutive materials and physicochemical characteristics, NPs may allow formulators to design different release profiles and to achieve local or systemic targeting of the encapsulated drug.41 Patents published on oral insulin delivery formulations have been recently reviewed.47

Currently, the clinicaltrial.gov registry lists two recruiting clinical studies aimed at evaluating safety/efficacy of oral insulin in patients with type 1 diabetes (NCT01973920, Oshadi Icp, Oshadi Drug Administration, and NCT02094534, ORMD-0801 capsules, Oramed Pharmaceutical/Hadassah Medical Organization), two completed studies (NCT01120912, Oshadi oral insulin, and NCT00867594, ORMD-0801), and one unknown study (NCT01035801, Drug: IN-105, Biocon Limited).

In human type 1 diabetes, the administration dose of oral insulin reported in the literature ranged widely, from 50 units to 2,400 units.4851 The addition of ORMD-0801 oral insulin capsules (8 mg insulin) three times daily to subcutaneous insulin injection regimens of eight patients with type 1 diabetes resulted in a synergistic reduction in blood glucose concentrations, which was most prominent during the early evening hours.48 Kapitza et al investigated the pharmacokinetic and pharmacodynamic properties of an oral insulin formulation (300 units combined with 400 mg monosodium N-(4-chlorosalicyloyl)-4-aminobutyrate in two capsules) and compared it with subcutaneously injected regular human insulin in ten patients with type 2 diabetes. Maximum plasma insulin concentration was significantly higher, and time was significantly shorter with oral insulin administration. Relative bioavailability of oral insulin for the 0–1-hour, 0–2-hour, and 0–6-hour periods were 26%±28%, 7%±4%, and 2%±1%, respectively. Respective values for biopotency were 55%±92%, 12%±9%, and 3%±1%.49 Under fasting conditions, variability in absorption was high (coefficient of variation 60%–70%), but could increase further with prandial administration.49 The time-action of another orally administered enteric insulin capsule formulation (insulin doses 50 units, 100 units, and 200 units) was evaluated in 12 healthy Chinese subjects using euglycemic glucose clamps. The mean time periods for maximal metabolic effects for 50 units, 100 units, and 200 units were 250±118 minutes, 170±58 minutes, and 236±132 minutes, respectively; the onset of action was at 38±10 minutes, 41±18 minutes, and 65±58 minutes, respectively. Thus, the time-action profile was similar to that of the comparator NPH insulin. The biopotency of this formulation was uncertain due to the high variability.50 A Phase I clinical trial assessed the safety and tolerability of single ascending doses of oral insulin 106 (NNC 0148-0000-0106) in healthy subjects, and of a single-dose level of oral insulin 106 in subjects with type 1 and type 2 diabetes. Insulin 106 in a GIPET® I gastro-resistant tablet was administered orally as tablets of 300 nmol, 900 nmol, 1,800 nmol, 3,600 nmol, and 7,200 nmol. The time to maximum serum concentration ranged from 0.75 hours to 1.88 hours, and the mean serum half-life values increased from 0.64 hours to 2.17 hours over the tested dose range in fasting state. The interindividual variability in the pharmacokinetic endpoints of oral insulin 106 was large with a coefficient of variation of ~200% for its area under the serum insulin concentration time curve. The pharmacodynamic results demonstrated a rapid onset of action with a large interindividual variability in glucose infusion rate endpoints (coefficient of variation 95%–445%).51

Carriers for oral delivery of insulin

Most materials used in the formulation of oral insulin NPs and tested in animals were polymers. Hydrophilic or hydrophobic polymers often were synthesized as microparticles or NPs. Polymers such as poly(lactide-co-glycolide (PLGA), poly(lactide) (PLA), poly(ε-caprolactone) (PCL), chitosan (CS) and its derivatives, dextran, solid lipids, poly(allylamine), and poly(acrylic acid) have been used due to their well-established safety. Using various polymeric materials and formulation processes allows to modulate the physicochemical properties of NPs, extent of drug loading, and drug release profile.40

The most widely used polymeric materials were based on PLGA as poly-hydroxyethyl-aspartamide,52 cyclodextrin and polymethacrylic acid,53 PLGA/HP55,54 concanavalin A-PLGA conjugate,55 antacid co-encapsulated PLGA,56 folate-decorated PLGA NPs,57 insulin-sodium oleate-PLGA complex.58 Chitosan-based materials were formulated as chitosan–insulin NPs5961 or conjugated with alginate,6264 poly(γ-glutamic acid),65 lauryl succinyl,66 poly(methyl methacrylate),67 and iron oxide NPs.68 Solid lipid NPs containing insulin were formulated with cetyl palmitate69 and stearic acid–octaarginine70 in order to protect insulin from enzymes. Many of these polymeric NPs were effective in lowering the blood glucose level in animal models; however, much larger doses of insulin were required in comparison with subcutaneous administration. Using some of these promising NP systems, insulin doses required to reduce blood glucose concentration by 50% range from 30 IU/kg to 100 IU/kg; they are higher than the typical dose of 1 IU/kg needed to induce the same reduction in blood glucose level via the subcutaneous injection.40 Moreover, it has been suggested that long-term administration of high doses of insulin may induce mitogenic changes in the gastrointestinal epithelium, as insulin is also a growth factor.40 Thus, the current polymer NPs should be improved in order to reduce the amount of insulin required to control blood glucose levels.40

Gold nanoparticles (AuNPs) are being investigated as novel carriers for oral insulin delivery owing to their excellent biocompatibility and low cytotoxicity. AuNPs are stable metal NPs with unique physical, chemical, optical, and electronic properties: large surface-to-volume ratio for easy conjugation of a variety of ligands, practical nanoscale assembly, inert nature, extreme resistance to oxidation, enhanced permeability and retention effect, surface plasmon resonance phenomenon, size- and shape-dependent electronic, and optical properties. To use the AuNPs in vivo for a long retention time avoiding the action of the reticular endothelial system, their surface can be modified with antibiofouling agents, such as polyethylene glycol, and more stable bonds can be created by self-assembling molecules with thiol groups onto gold surfaces.71

In 2006, insulin-capped bare AuNPs (Au-Ins) and aspartic acid-capped AuNPs (Au-Asp-Ins) were delivered in diabetic Wistar rats by both oral and intranasal (transmucosal) routes and significantly lowered blood glucose levels.72 Oral administration of both Au-Ins (50 IU/kg) and Au-Asp-Ins (50 IU/kg) formulations reduced blood glucose levels by 19% and 31%, respectively. Nasal administration of both Au-Ins (20 IU/kg) and Au-Asp-Ins (20 IU/kg) induced a maximum reduction of 50% and 55%, respectively. Moreover, the formulation Au-Ins released insulin more slowly than the Au-Asp-Ins formulation. The maximum blood glucose reduction occurred 180 minutes and 120 minutes after administration of the Au-Ins and the Au-Asp-Ins formulations, respectively. Thus, by comparing the maximum blood glucose-reducing response to the two formulations (oral and intranasal), the insulin uptake was higher and faster in the intranasal delivery protocol. Membrane permeability of nanogold-insulin formulations across nasal mucosal cells was greater than across gastrointestinal mucosa. Indeed, the diabetic rats received 20 IU/kg of insulin in the intranasal experiment vs 50 IU/kg after oral administration. The maximum blood glucose reduction after the Au-Asp-Ins intranasal administration (55%) was comparable to that observed after the subcutaneous administration (53%). This finding suggested that the transmucosal delivery of insulin by AuNPs could be an effective alternative to subcutaneous delivery.72

Recently, chondroitin sulfate (CS)-capped AuNPs were synthesized. CS, a sulfated glycosamino-glycan composed of N-acetylgalactosamine and glucuronic acid, was used as a stabilizer in the synthesis of AuNPs, and insulin was embedded in the CS-capped AuNPs structure (AuNPs/INS, ~120 nm mean diameter, narrow size distribution, and negative zeta potential).73 AuNPs/INS remained stable during the test period and its cytotoxicity against Caco-2 cells was negligible. In the diabetic rat model, oral administration of the AuNPs/INS formulation reduced blood glucose level up to 32.1%. The reduction in the blood glucose level was higher than previous reported results using insulin-loaded sodium borohydride reduced AuNPs (18%).72 Moreover, the mean insulin concentration 120 minutes after oral administration of AuNP/INS was 6.61-fold higher than that of insulin solution-treated group, thus suggesting that AuNPs had a role in the permeation of insulin.

Thus, several NP systems have been developed for oral insulin delivery with promising results in experimental models, but their long-term efficacy and safety must be demonstrated in animals and human beings. Furthermore, so far, the low bioavailability showed by various noninvasive insulin delivery have required huge insulin doses as compared to subcutaneously injected insulin: the additional cost adversely affects cost-effectiveness as with inhaled insulin. Finally, the management of both fasting and postprandial blood glucose levels requires different time-action profiles of oral formulations (rapid and basal) that ensure the predictability and reproducibility of insulin release. No oral insulin formulation is commercially available, and there have been very few clinical trial reports with human data.

Conclusion and future strategies

Oral delivery of insulin has the chance to improve the quality of life of patients with diabetes. There are therefore several attempts to develop oral carrier systems to resist gastrointestinal tract conditions and to preserve insulin integrity. The literature suggests that insulin carriers based on biodegradable polymers and AuNPs are promising perspectives to prepare formulations for oral insulin delivery. However, current research efforts are still allocated to the preclinical stages and clinical data reports only made up 4% of all the oral insulin publications. From the pharmacological point of view, the main barriers faced in oral insulin delivery are 1) unpredictable and erratic absorption and 2) the extremely low and variable bioavailability and bioefficacy. Furthermore, improved clinical trial designs should consider short-term and long-term outcomes, active comparators, the effect of food on insulin absorption, and response reproducibility.74

Noninvasive insulin delivery is an attractive target to reduce the perceived burden of conventional subcutaneous insulin treatment. Developing efficient drug delivery systems requires large-scale, multidisciplinary research efforts that combine biological and polymer sciences, pharmaceutical biotechnology, and conjugation chemistry. Research initiatives should focus on promoting most promising projects coordinated between national and international programs through a joint effort of governments, universities, and industries. From the manufacturing point of view, the production of safety materials is essential for sustainable development of oral insulin formulations. The investigation of the toxicological features and the impact of nanomaterials on the body as well as on the environment are important aspects, which cannot be overlooked.75

Disclosure

The authors report no conflicts of interest in this work.


References

1.

Yeh HC, Brown TT, Maruthur N, et al. Comparative effectiveness and safety of methods of insulin delivery and glucose monitoring for diabetes mellitus: a systematic review and meta-analysis. Ann Intern Med. 2012;157:336–347.

2.

Yeh HC, Lau BD, Golden SH, Donner T, Brown TT, Bass EB. Insulin Delivery and Glucose Monitoring Methods: Future Research Needs: Identification of Future Research Needs From Comparative Effectiveness Review No 57. Rockville, MD: Agency for Healthcare Research and Quality (US); 2013.

3.

Jeitler K, Horvath K, Berghold A, et al. Continuous subcutaneous insulin infusion versus multiple daily insulin injections in patients with diabetes mellitus: systematic review and meta-analysis. Diabetologia. 2008;51:941–951.

4.

Rorsman P, Braun M. Regulation of insulin secretion in human pancreatic islets. Annu Rev Physiol. 2013;75:155–179.

5.

Pørksen N, Grøfte T, Greisen J, et al. Human insulin release processes measured by intraportal sampling. Am J Physiol Endocrinol Metab. 2002;282(3):E695–E702.

6.

Tura A, Pacini G, Kautzky-Willer A, Ludvik B, Prager R, Thomaseth K. Basal and dynamic proinsulin-insulin relationship to assess beta-cell function during OGTT in metabolic disorders. Am J Physiol Endocrinol Metab. 2003;285(1):E155–E162.

7.

Caumo A, Luzi L. First-phase insulin secretion: does it exist in real life? Considerations on shape and function. Am J Physiol Endocrinol Metab. 2004;287(3):E371–E385.

8.

Meier JJ, Veldhuis JD, Butler PC. Pulsatile insulin secretion dictates systemic insulin delivery by regulating hepatic insulin extraction in humans. Diabetes. 2005;54(6):1649–1656.

9.

Edgerton DS, Lautz M, Scott M, et al. Insulin’s direct effects on the liver dominate the control of hepatic glucose production. J Clin Invest. 2006;116(2):521–527.

10.

Aronoff SL, Berkowitz KB, Sheiner B, Want L. Glucose metabolism and regulation: beyond insulin and glucagon. Diabetes Spectr. 2004;17:183–190.

11.

LeRoith D, Taylor SI, Olefsky JM. Diabetes Mellitus: A Fundamental and Clinical Text. 3rd ed. Philadelphia: Lippincott Williams & Wilkins; 2004.

12.

Ferrannini E. Physiology of glucose homeostasis and insulin therapy in type 1 and type 2 diabetes. Endocrinol Metab Clin North Am. 2012;41(1):25–39.

13.

Verma A, Kuma N, Malviya R, Sharma PK. Emerging trends in noninvasive insulin delivery. J Pharm. 2014;2014:1–9. [article ID 378048].

14.

Elçioglu HK, Sezer AD. Nanoparticle insulin drug delivery – applications and new aspects. In: Sezer AD, editor. Application of Nanotechnology in Drug Delivery. Rijeka: InTech; 2014:237–256.

15.

Lee MY, Shin MC, Yang VC. Transcutaneous antigen delivery system. BMB Rep. 2013;46(1):17–24.

16.

Prausnitz MR, Langer R. Transdermal drug delivery. Nat Biotechnol. 2008;26(11):1261–1268.

17.

Tuan-Mahmood TM, McCrudden MT, Torrisi BM, et al. Microneedles for intradermal and transdermal drug delivery. Eur J Pharm Sci. 2013;50(5):623–637.

18.

Andrews SN, Jeong E, Prausnitz MR. Transdermal delivery of molecules is limited by full epidermis, not just stratum corneum. Pharm Res. 2013;30(4):1099–1109.

19.

Lee S, McAuliffe DJ, Mulholland SE, Doukas AG. Photomechanical transdermal delivery of insulin in vivo. Lasers Surg Med. 2001;28(3):282–285.

20.

Fang JY, Lee WR, Shen SC, Wang HY, Fang CL, Hu CH. Transdermal delivery of macromolecules by erbium: YAG laser. J Control Rel. 2004;100(1):75–85.

21.

Nguyen TM, Lee S, Lee SB. Conductive polymer nanotube patch for fast and controlled ex vivo transdermal drug delivery. Nanomedicine (Lond). 2014;9(15):2263–2272.

22.

Kajimoto K, Yamamoto M, Watanabe M, et al. Noninvasive and persistent transfollicular drug delivery system using a combination of liposomes and iontophoresis. Int J Pharm. 2011;403(1–2):57–65.

23.

Malakar J, Sen SO, Nayak AK, Sen KK. Formulation, optimization and evaluation of transferosomal gel for transdermal insulin delivery. Saudi Pharm J. 2012;20(4):355–363.

24.

Ling MH, Chen MC. Dissolving polymer microneedle patches for rapid and efficient transdermal delivery of insulin to diabetic rats. Acta Biomater. 2013;9(11):8952–8961.

25.

McVey E, Hirsch L, Sutter DE, et al. Pharmacokinetics and postprandial glycemic excursions following insulin lispro delivered by intradermal microneedle or subcutaneous infusion. J Diabetes Sci Technol. 2012;6(4):743–754.

26.

Mastrandrea LD. Inhaled insulin: overview of a novel route of insulin administration. Vasc Health Risk Manag. 2010;6:47–58.

27.

McElroy MC, Kirton C, Gliddon D, Wolff RK. Inhaled biopharmaceutical drug development: nonclinical considerations and case studies. Inhal Toxicol. 2013;25(4):219–232.

28.

Dolovich MB, Dhand R. Aerosol drug delivery: developments in device design and clinical use. Lancet. 2011;377(9770):1032–1045.

29.

Bailey CJ, Barnett AH. Why is Exubera being withdrawn? BMJ. 2007;335:1156.

30.

Kugler AJ, Fabbio KL, Pham DQ, Nadeau DA. Inhaled Technosphere insulin: a novel delivery system and formulation for the treatment of types 1 and 2 diabetes mellitus. Pharmacotherapy. 2015;35(3):298–314.

31.

Fineberg SE, Kawabata TT, Krasner AS, Fineberg NS. Insulin antibodies with pulmonary delivery of insulin. Diabetes Technol Ther. 2007;9(suppl 1):S102–S110.

32.

Garg SK, Mathieu C, Rais N, et al. Two-year efficacy and safety of AIR inhaled insulin in patients with type 1 diabetes: an open-label randomized controlled trial. Diabetes Technol Ther. 2009;11(suppl 2):S5–S16.

33.

Raskin P, Heller S, Honka M, et al. Pulmonary function over 2 years in diabetic patients treated with prandial inhaled Technosphere insulin or usual antidiabetes treatment: a randomized trial. Diabetes Obes Metab. 2012;14(2):163–173.

34.

Rave K, Bott S, Heinemann L, et al. Time-action profile of inhaled insulin in comparison with subcutaneously injected insulin lispro and regular human insulin. Diabetes Care. 2005;28(5):1077–1082.

35.

Forst T, Hohberg C, Schöndorf T, et al. Time-action profile and patient assessment of inhaled insulin via the Exubera device in comparison with subcutaneously injected insulin aspart via the FlexPen device. Diabetes Technol Ther. 2009;11(2):87–92.

36.

Quattrin T, Belanger A, Bohannon NJ, Schwartz SL. Efficacy and safety of inhaled insulin (Exubera) compared with subcutaneous insulin therapy in patients with type 1 diabetes: results of a 6-month, randomized, comparative trial. Diabetes Care. 2004;27(11):2622–2627.

37.

Skyler JS, Hollander PA, Jovanovic L, et al; Inhaled Human Insulin Type 1 Diabetes Study Group. Safety and efficacy of inhaled human insulin (Exubera) during discontinuation and readministration of therapy in adults with type 1 diabetes: a 3-year randomized controlled trial. Diabetes Res Clin Pract. 2008;82(2):238–246.

38.

Moses RG, Bartley P, Lunt H, et al. Safety and efficacy of inhaled insulin (AERx iDMS) compared with subcutaneous insulin therapy in patients with Type 1 diabetes: 1-year data from a randomized, parallel group trial. Diabet Med. 2009;26(3):260–267.

39.

Comulada AL, Renard E, Nakano M, et al. Efficacy and safety of AIR inhaled insulin compared to insulin lispro in patients with type 1 diabetes mellitus in a 6-month, randomized, noninferiority trial. Diabetes Technol Ther. 2009;11(suppl 2):S17–S25.

40.

Chen MC, Sonaje K, Chen KJ, Sung HW. A review of the prospects for polymeric nanoparticle platforms in oral insulin delivery. Biomaterials. 2011;32:9826–9838.

41.

Diab R, Jaafar-Maalej C, Fessi H, Maincent P. Engineered nanoparticulate drug delivery systems: the next frontier for oral administration? AAPS J. 2012;14(4):688–702.

42.

Patel A, Cholkar K, Mitra AK. Recent developments in protein and peptide parenteral delivery approaches. Ther Deliv. 2014;5(3):337–365.

43.

Woitiski CB, Neufeld RJ, Veiga F, Carvalho RA, Figueiredo IV. Pharmacological effect of orally delivered insulin facilitated by multilayered stable nanoparticles. Eur J Pharm Sci. 2010;41(3–4):556–563.

44.

Damge C, Socha M, Ubrich N, Maincent P. Poly(epsilon-caprolactone)/eudragit nanoparticles for oral delivery of aspart-insulin in the treatment of diabetes. J Pharm Sci. 2010;99(2):879–889.

45.

Sonaje K, Lin KJ, Wey SP, et al. Biodistribution, pharmacodynamics and pharmacokinetics of insulin analogues in a rat model: oral delivery using pH-responsive nanoparticles vs subcutaneous injection. Biomaterials. 2010;31(26):6849–6858.

46.

Fonte P, Araujo F, Reis S, Sarmento B. Oral insulin delivery: how far are we? J Diabetes Sci Technol. 2013;7(2):520–531.

47.

Navgire SD, Satpute AS, Pandey S, Patil AT. Recent patents on oral insulin delivery. Recent Pat Drug Deliv Formul. 2014;8(3):202–205.

48.

Eldor R, Arbit E, Corcos A, Kidron M. Glucose-reducing effect of the ORMD-0801 oral insulin preparation in patients with uncontrolled type 1 diabetes: a pilot study. PLoS One. 2013;8(4):e59524.

49.

Kapitza C, Zijlstra E, Heinemann L, Castelli MC, Riley G, Heise T. Oral insulin: a comparison with subcutaneous regular human insulin in patients with type 2 diabetes. Diabetes Care. 2010;33(6):1288–1290.

50.

Li J, Wang Y, Han L, Sun X, Yu H, Yu Y. Time-action profile of an oral enteric insulin formulation in healthy Chinese volunteers. Clin Ther. 2012;34(12):2333–2338.

51.

Novo Nordisk. A Trial Investigating the Safety, Tolerability, Pharmacokinetics and Pharmacodynamics of NNC 0148-0000-0106 in Healthy Subjects and Subjects with Type 1 and Type 2 Diabetes; 2012. Available from: http://novotrials.com/website/pdf/registry/bin_20120423-015136-794.pdf.

52.

Licciardi M, Pitarresi G, Cavallaro G, Giammona G. Nanoaggregates based on new poly-hydroxyethyl-aspartamide copolymers for oral insulin absorption. Mol Pharm. 2013;10(5):1644–1654.

53.

Sajeesh S, Sharma CP. Cyclodextrin-insulin complex encapsulated polymethacrylic acid based nanoparticles for oral insulin delivery. Int J Pharm. 2006;325(1–2):147–154.

54.

Wu ZM, Ling L, Zhou LY, et al. Novel preparation of PLGA/HP55 nanoparticles for oral insulin delivery. Nanoscale Res Lett. 2012;7(1):299.

55.

Hurkat P, Jain A, Jain A, Shilpi S, Gulbake A, Jain SK. Concanavalin A conjugated biodegradable nanoparticles for oral insulin delivery. J Nanopart Res. 2012;14:1219.

56.

Sharma G, van der Walle CF, Ravi Kumar MN. Antacid co-encapsulated polyester nanoparticles for peroral delivery of insulin: development, pharmacokinetics, biodistribution and pharmacodynamics. Int J Pharm. 2013;440:99–110.

57.

Jain S, Rathi VV, Jain AK, Das M, Godugu C. Folate-decorated PLGA nanoparticles as a rationally designed vehicle for the oral delivery of insulin. Nanomedicine (Lond). 2012;7:1311–1337.

58.

Sun S, Liang N, Piao H, Yamamoto H, Kawashima Y, Cui F. Insulin-S.O (sodium oleate) complex-loaded PLGA nanoparticles: formulation, characterization and in vivo evaluation. J Microencapsul. 2010;27(6):471–478.

59.

Mukhopadhyay P, Sarkar K, Chakraborty M, Bhattacharya S, Mishra R, Kundu PP. Oral insulin delivery by self-assembled chitosan nanoparticles: in vitro and in vivo studies in diabetic animal model. Mater Sci Eng C Mater Biol Appl. 2013;33:376–382.

60.

Elsayed A, Al-Remawi M, Qinna N, Farouk A, Al-Sou’od KA, Badwan AA. Chitosan-sodium lauryl sulfate nanoparticles as a carrier system for the in vivo delivery of oral insulin. AAPS Pharm Sci Tech. 2011;12(3):958–964.

61.

Makhlof A, Tozuka Y, Takeuchi H. Design and evaluation of novel pH-sensitive chitosan nanoparticles for oral insulin delivery. Eur J Pharm Sci. 2011;42(5):445–451.

62.

Sarmento B, Ribeiro A, Veiga F, Sampaio P, Neufeld R, Ferreira D. Alginate/chitosan nanoparticles are effective for oral insulin delivery. Phar Res. 2007;24(12):2198–2206.

63.

Kadir A, Mokhtar MT, Wong TW. Nanoparticulate assembly of mannuronic acid- and guluronic acid-rich alginate: oral insulin carrier and glucose binder. J Pharm Sci. 2013;102(12):4353–4363.

64.

Paul W, Sharma CP. Synthesis and characterization of alginate coated zinc calcium phosphate nanoparticles for intestinal delivery of insulin. Process Biochem. 2012;47:882–886.

65.

Sonaje K, Chen YJ, Chen HL, et al. Enteric-coated capsules filled with freeze-dried chitosan/poly(gamma-glutamic acid) nanoparticles for oral insulin delivery. Biomaterials. 2010;31(12):3384–3394.

66.

Rekha MR, Sharma CP. Synthesis and evaluation of lauryl succinyl chitosan particles towards oral insulin delivery and absorption. J Control Release. 2009;135(2):144–151.

67.

Cui F, Qian F, Zhao Z, Yin L, Tang C, Yin C. Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles. Biomacromolecules. 2009;10(5):1253–1258.

68.

Kebede A, Singh AK, Rai PK, et al. Controlled synthesis, characterization, and application of iron oxide nanoparticles for oral delivery of insulin. Lasers Med Sci. 2013;28(2):579–587.

69.

Sarmento B, Martins S, Ferreira D, Souto EB. Oral insulin delivery by means of solid lipid nanoparticles. Int J Nanomedicine. 2007;2(4):743–749.

70.

Zhang ZH, Zhang YL, Zhou JP, Lv HX. Solid lipid nanoparticles modified with stearic acid-octaarginine for oral administration of insulin. Int J Nanomedicine. 2012;7:3333–3339.

71.

Ruggeri G, Covolan VL, Bernabò M, et al. Metal nanostructures with magnetic and biodegradable properties for medical applications. J Braz Chem Soc. 2013;24:191–200.

72.

Joshi HM, Bhumkar DR, Joshi K, Pokharkar V, Sastry M. Gold nanoparticles as carriers for efficient transmucosal insulin delivery. Langmuir. 2006;22(1):300–305.

73.

Cho HJ, Oh J, Choo MK, Ha JI, Park Y, Maeng HJ. Chondroitin sulfate-capped gold nanoparticles for the oral delivery of insulin. Int J Biol Macromol. 2014;63:15–20.

74.

Zijlstra E, Heinemann L, Plum-Morschel L. Oral insulin reloaded: a structured approach. J Diabetes Sci Technol. 2014;8(3):458–465.

75.

Bakshi M, Singh HB, Abhilash PC. The unseen impact of nanoparticles: more or less? Curr Sci. 2014;106:350–352.

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