skip to content
Dovepress - Open Access to Scientific and Medical Research
View our mobile site

8852

In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles

(2552) Views  (697) Full article downloads

Authors: D Nagesha, GS Laevsky, P Lampton, R Banyal, C Warner

Published Date January 2007 Volume 2007:2(4) Pages 813 - 819
DOI: http://dx.doi.org/10.2147/IJN.S

D Nagesha1, GS Laevsky2, P Lampton3, R Banyal1, C Warner3, C DiMarzio2, S Sridhar1

1Electronic Materials Research Institute and Department of Physics, 2Electrical and Computer Engineering Department, 3Department of Biology, Northeastern University, Boston, MA, USA

Abstract: Recent advances in nonlinear optical techniques and materials such as quantum wells, nanowires and noble-metal nanoparticles have led to advances in cellular imaging wherein various nanoparticles have been shown to improve both in vitro and in vivo visualization. In this paper, we demonstrate in vitro imaging using multi-photon photoluminescence of gold nanoparticles from two different cell types – Dictyostelium discoideum and mouse embryonic stem cells. By observing nanoparticles we show that embryonic stem cells maintained their ability to proliferate for several passages while grown in the presence of gold nanoparticles. The advantages of multi-photon luminescence using gold nanoparticles have important implications for use in stem cell proliferation experiments and in vitro experiments to monitor differentiation.

Keywords: gold nanoparticles, multiphton absorption induced luminescence, mouse embryonic stem cells, visualization








Readers of this article also read:

Selective placement of templated DNA nanowires between microstructured electrodes
Adverse effects of fullerenes on endothelial cells: Fullerenol C60(OH)24 induced tissue factor and ICAM-1 membrane expression and apoptosis in vitro
Nanocarriers as pulmonary drug delivery systems to treat and to diagnose respiratory and non respiratory diseases
Evidence-based decision-making within the context of globalization: A “Why–What–How” for leaders and managers of health care organizations
Radiolucency below the crown of mandibular horizontal incompletely impacted third molars and acute inflammation in men with diabetes
"Globalized public health.” A transdisciplinary comprehensive framework for analyzing contemporary globalization’s influences on the field of public health
The pathophysiology of bronchiectasis
Long-term treatment of bipolar disorder with a radioelectric asymmetric conveyor
Comparison of two treatments for coxarthrosis: local hyperthermia versus radio electric asymmetrical brain stimulation
Radio electric asymmetric brain stimulation in the treatment of behavioral and psychiatric symptoms in Alzheimer disease