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Glutaric acid as a spacer facilitates improved intracellular uptake of LHRH–SPION into human breast cancer cells

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Authors: Challa SSR Kumar, Carola Leuschner, Michelle Urbina, Tevhida Ozkaya, Josef Hormes

Published Date August 2007 Volume 2007:2(2) Pages 175 - 179
DOI: http://dx.doi.org/10.2147/IJN.S

Challa SSR Kumar1, Carola Leuschner3, Michelle Urbina1,2, Tevhida Ozkaya1,4, Josef Hormes1

1Center for Advanced Microstructures and Devices, Louisiana State University, Baton Rouge, LA, USA; 2Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, LA, USA; 3Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA; 4Chemistry Department, Fatih University, Istanbul, Turkey

Abstract: Superparamagnetic iron oxide nanoparticles (SPIONs) bound directly to luteinizing hormone releasing hormone (LHRH) have shown high efficiency for intracellular uptake to breast cancer cells, MDA-MB-435S.luc. We demonstrate in this communication that inclusion of a small spacer molecule such as glutaric acid (Glu) in between SPION and LHRH increases further receptor mediated intracellular uptake. LHRH-bound SPIONs with and without the spacer molecule were nontoxic.

Keywords: Glutaric acid; luteinizing hormone releasing hormone; superparamagnetic iron oxide nanoparticles, MDA-MB-435S.luc., intracellular uptake