Gene polymorphisms in association with self-reported stroke in US adults

Original Research

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Authors: Amy Z Fan, Jing Fang, Ajay Yesupriya, et al

Published Date March 2010 Volume 2010:3 Pages 23 - 28

DOI: http://dx.doi.org/10.2147/TACG.S8574

Amy Z Fan¹, Jing Fang¹, Ajay Yesupriya², Man-huei Chang², Greta Kilmer¹, Meaghan House³, Donald Hayes¹, Renée M Ned², Nicole F Dowling², Ali H Mokdad¹

¹National Center for Chronic Disease Prevention and Health Promotion, Centers for Disease Control and Prevention, Atlanta, GA, USA; ²Office of Public Health Genomics, Coordinating Center for Health Promotion, Centers for Disease Control and Prevention, Atlanta, GA, USA; ³School of Public Health, Emory University, Atlanta, GA, USA

Purpose: Epidemiologic studies suggest that several gene variants increase the risk of stroke, and population-based studies help provide further evidence. We identified polymorphisms associated with the prevalence of self-reported stroke in US populations using a representative sample.

Methods: Our sample comprised US adults in the Third National Health and Nutrition Examination (NHANES III) DNA bank. We examined nine candidate gene variants within ACE, F2, F5, ITGA2, MTHFR, and NOS3 for associations with self-reported stroke. We used multivariate regression and Cox proportional hazards models to test the association between these variants and history of stroke.

Results: In regression models, the rs4646994 variant of ACE (I/I and I/D genotypes) was associated with higher prevalence adjusted prevalence odds ratio [APOR] = 2.66 [1.28, 5.55] and 2.23 [1.30, 3.85], respectively) compared with the D/D genotype. The heterozygous genotype of MTHFR rs1801131 (A/C) was associated with lower prevalence of stroke (APOR = 0.48 [0.25, 0.92]) compared with A/A and C/C genotypes. For rs2070744 of NOS3, both the C/T genotype (APOR = 1.91 [1.12, 3.27]) and C/C genotype (APOR = 3.31 [1.66, 6.60]) were associated with higher prevalence of stroke compared with the T/T genotype.

Conclusion: Our findings suggest an association between the prevalence of self-reported stroke and polymorphisms in ACE, MTHFR, and NOS3 in a population-based sample.

Keywords: stroke, gene, polymorphisms, NHANES III, gene association analysis