Back to Browse Journals » Neurobehavioral HIV Medicine » Volume 3

Expression of mannose binding lectin in HIV-1-infected brain: implications for HIV-related neuronal damage and neuroAIDS

Authors Singh KK, Nathamu S, Adame A, Alire TU, Dumaop W, Gouaux B, Moore DJ, Masliah E, and HNRC Group

Published Date May 2011 Volume 2011:3 Pages 41—52

DOI http://dx.doi.org/10.2147/NBHIV.S19969

Published 30 May 2011

Kumud K Singh1, Satyanarayana Nathamu1, Anthony Adame2, Tara U Alire1, Wilmar Dumaop2, Ben Gouaux3, David J Moore3, Eliezer Masliah2, and HIV Neurobehavioral Research Center Group
1Department of Pediatrics, 2Department of Neurosciences, 3Department of Psychiatry, University of California San Diego, La Jolla, CA, USA

Abstract: Mannose binding lectin (MBL) activates complement pathway that leads to pathogen opsonization and phagocytosis. MBL deficiency is linked to HIV transmission and disease progression. We sought to determine the role of MBL in HIV encephalitis (HIVE) by evaluating its presence and distribution in the HIV-1-infected brain and by assessing its association with monocyte chemoattractant protein-1 (MCP-1) expression. This retrospective study utilized archived post-mortem brain tissues obtained from 35 individuals enrolled in a longitudinal study as part of the California NeuroAIDS Tissue Network. MBL, MCP-1 and brain cell markers in post-mortem brain tissues with or without HIVE were evaluated using immunocytochemistry, immunofluorescence, confocal microscopy, and western blots. MBL was expressed in neurons, astrocytes, microglia, and oligodendrocytes of the frontal cortex of the HIV-1-infected brain. Overall, there were 30% to 40% more MBL-positive brain cells in HIVE vs non-HIVE cases (P = 0.01, paired t-test). Specifically, there was an increased MBL expression in the neuronal axons of HIVE cases. Also, western blots showed 3- to 4-fold higher levels of 78 kD MBL trimers in HIVE vs non-HIVE cases. This MBL-HIVE link was further confirmed by MBL associated higher MCP-1 expression in HIVE vs non-HIVE cases. HIV negative healthy individuals and normal or the gp120 transgenic mice did not show any differential MBL expression. Increased MBL expression in the major brain cell types, specifically in the neuronal axons of HIVE brain, and MBL associated higher MCP-1 expression in HIVE suggest that MBL could cause neuroinflammation and neuronal injury through MBL complement activation pathway.

Keywords: mannose binding lectin, HIV encephalitis, complement activation, neuroinflammation, neuroAIDS, MCP-1

Download Article [PDF] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php

Readers of this article also read:

Femtosecond laser versus mechanical microkeratome-assisted flap creation for LASIK: a prospective, randomized, paired-eye study

Pajic B, Vastardis I, Pajic-Eggspuehler B, Gatzioufas Z, Hafezi F

Clinical Ophthalmology 2014, 8:1883-1889

Published Date: 22 September 2014

Association of retinal vessel attenuation with visual function in eyes with retinitis pigmentosa

Nakagawa S, Oishi A, Ogino K, Makiyama Y, Kurimoto M, Yoshimura N

Clinical Ophthalmology 2014, 8:1487-1493

Published Date: 12 August 2014

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Cosco D, Federico C, Maiuolo J, Bulotta S, Molinaro R, Paolino D, Tassone P, Fresta M

International Journal of Nanomedicine 2014, 9:2359-2372

Published Date: 15 May 2014

Optic disc size and progression of visual field damage in patients with normal-tension glaucoma

Hayamizu F, Yamazaki Y, Nakagami T, Mizuki K

Clinical Ophthalmology 2013, 7:807-813

Published Date: 3 May 2013

Relationship between interblink interval and dopamine

Lemon TI, Shah RD

Clinical Ophthalmology 2013, 7:793-794

Published Date: 29 April 2013

Novel nanostructured biomaterials: implications for coronary stent thrombosis

Karagkiozaki V, Karagiannidis PG, Kalfagiannis N, Kavatzikidou P, Patsalas P, Georgiou D, Logothetidis S

International Journal of Nanomedicine 2012, 7:6063-6076

Published Date: 17 December 2012

Impact of surface coating and particle size on the uptake of small and ultrasmall superparamagnetic iron oxide nanoparticles by macrophages

Saito S, Tsugeno M, Koto D, Mori Y, Yoshioka Y, Nohara S, Murase K

International Journal of Nanomedicine 2012, 7:5415-5421

Published Date: 10 October 2012

Measurement of ocular surface protection under natural blink conditions

Abelson R, Lane KJ, Angjeli E, Johnston P, Ousler G, Montgomery D

Clinical Ophthalmology 2011, 5:1349-1357

Published Date: 22 September 2011