Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 4

In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles

Authors D Nagesha, GS Laevsky, P Lampton, R Banyal, C Warner

Published Date January 2007 Volume 2007:2(4) Pages 813—819

DOI http://dx.doi.org/

Published 28 January 2007

D Nagesha1, GS Laevsky2, P Lampton3, R Banyal1, C Warner3, C DiMarzio2, S Sridhar1

1Electronic Materials Research Institute and Department of Physics, 2Electrical and Computer Engineering Department, 3Department of Biology, Northeastern University, Boston, MA, USA

Abstract: Recent advances in nonlinear optical techniques and materials such as quantum wells, nanowires and noble-metal nanoparticles have led to advances in cellular imaging wherein various nanoparticles have been shown to improve both in vitro and in vivo visualization. In this paper, we demonstrate in vitro imaging using multi-photon photoluminescence of gold nanoparticles from two different cell types – Dictyostelium discoideum and mouse embryonic stem cells. By observing nanoparticles we show that embryonic stem cells maintained their ability to proliferate for several passages while grown in the presence of gold nanoparticles. The advantages of multi-photon luminescence using gold nanoparticles have important implications for use in stem cell proliferation experiments and in vitro experiments to monitor differentiation.

Keywords: gold nanoparticles, multiphton absorption induced luminescence, mouse embryonic stem cells, visualization

Download Article [PDF] 

Readers of this article also read:

Disease burden and patient reported outcomes among patients with moderate to severe psoriasis: an ethnography study

Narayanan S, Guyatt V, Franceschetti A, Hautamaki EL

Psoriasis: Targets and Therapy 2015, 5:1-7

Published Date: 23 December 2014

Lumbosacral plexus delineation, dose distribution, and its correlation with radiation-induced lumbosacral plexopathy in cervical cancer patients

Tunio M, Al Asiri M, Bayoumi Y, Abdullah O Balbaid A, AlHameed M, Gabriela SL, Amir O Ali A

OncoTargets and Therapy 2015, 8:21-27

Published Date: 23 December 2014

Vincristine sulfate liposomal injection for acute lymphoblastic leukemia [Corrigendum]

Soosay Raj TA, Smith AM, Moore AS

International Journal of Nanomedicine 2013, 8:4705-4706

Published Date: 5 December 2013

The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice

Lee S, Kim MS, Lee D, Kwon TK, Khang D, Yun HS, Kim SH

International Journal of Nanomedicine 2013, 8:147-158

Published Date: 7 January 2013

Application of magnetically induced hyperthermia in the model protozoan Crithidia fasciculata as a potential therapy against parasitic infections

Grazú V, Silber AM, Moros M, Asín L, Torres TE, Marquina C, Ibarra MR, Goya GF

International Journal of Nanomedicine 2012, 7:5351-5360

Published Date: 8 October 2012

Corrigendum

Chen ZQ, Liu Y, Zhao JH, Wang L, Feng NP

International Journal of Nanomedicine 2012, 7:1709-1710

Published Date: 30 March 2012