Back to Browse Journals » International Journal of Nanomedicine » Volume 1 » Issue 1

Selective adhesion and mineral deposition by osteoblasts on carbon nanofiber patterns

Authors Dongwoo Khang, Michiko Sato, Rachel L Price, Alexander E Ribbe Thomas J Webster

Published Date January 2006 Volume 2006:1(1) Pages 65—72

DOI http://dx.doi.org/

Published 25 January 2006

Dongwoo Khang1, Michiko Sato4, Rachel L Price2, Alexander E Ribbe3
Thomas J Webster2,4

1Department of Physics, 2Weldon School of Biomedical Engineering, 3Purdue Laboratory of Chemical Nanotechnology and Departments of Chemistry, and 4School of Materials Engineering, Purdue

Abstract: In an effort to develop better orthopedic implants, osteoblast (bone-forming cells) adhesion was determined on microscale patterns (30 μm lines) of carbon nanofibers placed on polymer substrates. Patterns of carbon nanofibers (CNFs) on a model polymer (polycarbonate urethane [PCU]) were developed using an imprinting method that placed CNFs in selected regions. Results showed the selective adhesion and alignment of osteoblasts on CNF patterns placed on PCU. Results also showed greater attraction forces between fibronectin and CNF (compared with PCU) patterns using atomic force microscope force-displacement curves. Because fibronectin is a protein that mediates osteoblast adhesion, these results provide a mechanism of why osteoblast adhesion was directed towards CNF patterns. Lastly, this study showed that the directed osteoblast adhesion on CNF patterns translated to enhanced calcium phosphate mineral deposition along linear patterns of CNFs on PCU. Since CNFs are conductive materials, this study formulated substrates that through electrical stimulation could be used in future investigations to further promote osteoblasts to deposit anisotropic patterns of calcium containing mineral similar to that observed in long bones.

Keywords: carbon nanotubes, carbon nanofibers, osteoblasts, orthopedic, biomaterials, alignment

Download Article [PDF] 

Readers of this article also read:

Novel opportunities and challenges offered by nanobiomaterials in tissue engineering

Fabrizio Gelain

International Journal of Nanomedicine 2008, 3:415-424

Published Date: 10 August 2008

A study on the preparation and characterization of plasmid DNA and drug-containing magnetic nanoliposomes for the treatment of tumors

Wang Z-Y, Wang L, Zhang J, Li Y-T, Zhang D-S

International Journal of Nanomedicine 2011, 6:871-875

Published Date: 27 April 2011

Novel PEG-graft-PLA nanoparticles with the potential for encapsulation and controlled release of hydrophobic and hydrophilic medications in aqueous medium

Wang B, Jiang W, Yan H, Zhang X, Yang L, Deng L, Singh GK, Pan J

International Journal of Nanomedicine 2011, 6:1443-1451

Published Date: 7 July 2011

Preparation and evaluation of antimicrobial activity of nanosystems for the control of oral pathogens Streptococcus mutans and Candida albicans

Pupe CG, Villardi M, Rodrigues CR, Rocha HVA, Maia LC, de Sousa VP, Cabral LM

International Journal of Nanomedicine 2011, 6:2581-2590

Published Date: 26 October 2011

Oral fondaparinux: use of lipid nanocapsules as nanocarriers and in vivo pharmacokinetic study

Ramadan A, Lagarce F, Tessier-Marteau A, Thomas O, Legras P, Macchi L, Saulnier P, Benoit JP

International Journal of Nanomedicine 2011, 6:2941-2951

Published Date: 21 November 2011

Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering

Naghavi Alhosseini S, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A, Kargozar S, Jalali N

International Journal of Nanomedicine 2012, 7:25-34

Published Date: 4 January 2012

Critical appraisal of the role of davunetide in the treatment of progressive supranuclear palsy

Gold M, Lorenzl S, Stewart AJ, Morimoto BH, Williams DR, Gozes I

Neuropsychiatric Disease and Treatment 2012, 8:85-93

Published Date: 9 February 2012

Induction of cell death in a glioblastoma line by hyperthermic therapy based on gold nanorods

Fernandez Cabada T, Sanchez Lopez de Pablo C, Martinez Serrano A, del Pozo Guerrero F, Serrano Olmedo JJ, Ramos Gomez M

International Journal of Nanomedicine 2012, 7:1511-1523

Published Date: 21 March 2012