Back to Browse Journals » Vascular Health and Risk Management » Volume 4 » Issue 4

Characterizing blood microparticles: Technical aspects and challenges

Authors Arun S Shet

Published Date September 2008 Volume 2008:4(4) Pages 769—774

DOI http://dx.doi.org/10.2147/VHRM.S955

Published 4 September 2008

Arun S Shet

Department of Medicine, St. Johns Medical College and Hospital, St. Johns National Academy of Health Sciences, Bangalore, India

Abstract: Although long considered to be cellular debris, microparticles (MPs) are more recently considered refl ective of cellular stimulation, activation, and degeneration/apoptosis. MPs that arise from the cellular components of blood and the endothelial lining of blood vessels are referred to as blood MPs and by general consensus are small (≤1.5 μm), expose the anionic phospholipid (PL) phosphatidylserine (PS) on the outer leaflet of their membrane, and bear surface membrane antigens refl ecting their cellular origin. This brief review summarizes the different approaches used by several groups to study blood MPs. The aim of this article is to review the technical aspects of characterizing the morphological and functional properties of blood MPs with emphasis on the preanalytical and analytical variables involved in these studies.

Keywords: microparticles, flow cytometry, techniques

Download Article [PDF] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php

Readers of this article also read:

Modified supracricoid laryngectomy: oncological and functional outcomes in the elderly

Allegra E, Franco T, Trapasso S, Domanico R, La Boria A, Garozzo A

Clinical Interventions in Aging 2012, 7:475-480

Published Date: 8 November 2012

Functionalization of single-walled carbon nanotubes and their binding to cancer cells

Madani SY, Tan A, Dwek M, Seifalian AM

International Journal of Nanomedicine 2012, 7:905-914

Published Date: 22 February 2012

Engineering of silicon surfaces at the micro- and nanoscales for cell adhesion and migration control

Torres-Costa V, Martínez-Muñoz G, Sánchez-Vaquero V, Muñoz-Noval A, González-Méndez L, Punzón-Quijorna E, Gallach-Pérez D, Manso-Silván M, Climent-Font A, García-Ruiz JP, Martín-Palma RJ

International Journal of Nanomedicine 2012, 7:623-630

Published Date: 3 February 2012

Zinc oxide nanoparticles as selective killers of proliferating cells

Taccola L, Raffa V, Riggio C, Vittorio O, Iorio MC, Vanacore R, Pietrabissa A, Cuschieri A

International Journal of Nanomedicine 2011, 6:1129-1140

Published Date: 30 May 2011

Levodopa in the treatment of Parkinson’s disease: an old drug still going strong

Werner Poewe, Angelo Antonini, Jan CM Zijlmans, et al

Clinical Interventions in Aging 2010, 5:229-238

Published Date: 25 August 2010

Natriuretic peptides (BNP and NT-proBNP): measurement and relevance in heart failure

A Palazzuoli, M Gallotta, I Quatrini, et al

Vascular Health and Risk Management 2010, 6:411-418

Published Date: 26 May 2010

Potentials and limitations of microorganisms as renal failure biotherapeutics

Poonam Jain, Sapna Shah, Razek Coussa, Satya Prakash

Biologics: Targets and Therapy 2009, 3:233-243

Published Date: 18 May 2009

Intravitreal triamcinolone acetonide: Pattern of secondary intraocular pressure rise and possible risk factors

Ziad F Bashshur, Abdallah M Terro, Christelle P El Haibi, Akaber M Halawi, Alexandre Schakal, Baha’ N Noureddin

Clinical Ophthalmology 2008, 2:269-274

Published Date: 30 June 2008