Back to Browse Journals » Vascular Health and Risk Management » Volume 4 » Issue 4

Characterizing blood microparticles: Technical aspects and challenges

Authors Arun S Shet

Published Date September 2008 Volume 2008:4(4) Pages 769—774

DOI http://dx.doi.org/10.2147/VHRM.S955

Published 4 September 2008

Arun S Shet

Department of Medicine, St. Johns Medical College and Hospital, St. Johns National Academy of Health Sciences, Bangalore, India

Abstract: Although long considered to be cellular debris, microparticles (MPs) are more recently considered refl ective of cellular stimulation, activation, and degeneration/apoptosis. MPs that arise from the cellular components of blood and the endothelial lining of blood vessels are referred to as blood MPs and by general consensus are small (≤1.5 μm), expose the anionic phospholipid (PL) phosphatidylserine (PS) on the outer leaflet of their membrane, and bear surface membrane antigens refl ecting their cellular origin. This brief review summarizes the different approaches used by several groups to study blood MPs. The aim of this article is to review the technical aspects of characterizing the morphological and functional properties of blood MPs with emphasis on the preanalytical and analytical variables involved in these studies.

Keywords: microparticles, flow cytometry, techniques

Download Article [PDF] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php

Readers of this article also read:

Manganese-containing Prussian blue nanoparticles for imaging of pediatric brain tumors

Dumont MF, Yadavilli S, Sze RW, Nazarian J, Fernandes R

International Journal of Nanomedicine 2014, 9:2581-2595

Published Date: 23 May 2014

Biocompatibility of magnetic Fe3O4 nanoparticles and their cytotoxic effect on MCF-7 cells

Chen DZ, Tang QS, Li XD, Zhou XJ, Zang J, Xue WQ, Xiang JY, Guo CQ

International Journal of Nanomedicine 2012, 7:4973-4982

Published Date: 14 September 2012

Concentration- and time-dependent response of human gingival fibroblasts to fibroblast growth factor 2 immobilized on titanium dental implants

Ma Q, Wang W, Chu PK, Mei S, Ji K, Jin L, Zhang Y,

International Journal of Nanomedicine 2012, 7:1965-1976

Published Date: 17 April 2012

Effects of safflower seed extract on arterial stiffness

Katsuya Suzuki, Shigekazu Tsubaki, Masami Fujita, et al

Vascular Health and Risk Management 2010, 6:1007-1014

Published Date: 3 November 2010

Transradial approach to lower extremity interventions

Ravikiran Korabathina, Sidharth S Yadav, John T Coppola, et al

Vascular Health and Risk Management 2010, 6:503-509

Published Date: 1 July 2010

Arterial pulmonary hypertension in noncardiac intensive care unit

Mykola V Tsapenko, Arseniy V Tsapenko, Thomas BO Comfere, Girish K Mour, Sunil V Mankad, Ognjen Gajic

Vascular Health and Risk Management 2008, 4:1043-1060

Published Date: 2 September 2008

The importance of endothelin-1 for microvascular dysfunction in diabetes

Majid Kalani

Vascular Health and Risk Management 2008, 4:1061-1068

Published Date: 5 August 2008

Depression and smoking cessation: Does the evidence support psychiatric practice?

Anna Lembke, Kenasha Johnson, Charles DeBattista

Neuropsychiatric Disease and Treatment 2007, 3:487-493

Published Date: 8 October 2007

Short-term effect of the HMG-CoA reductase inhibitor rosuvastatin on erythrocyte nitric oxide synthase activity

Barbara Ludolph, Wilhelm Bloch, Malte Kelm, Rainer Schulz, Petra Kleinbongard

Vascular Health and Risk Management 2007, 3:1069-1073

Published Date: 15 January 2007