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ZNF418 overexpression protects against gastric carcinoma and prompts a good prognosis

Authors Hui H, Hu Z, Jiang C, Wu J, Gao Y, Wang X

Received 25 December 2017

Accepted for publication 19 March 2018

Published 11 May 2018 Volume 2018:11 Pages 2763—2770

DOI https://doi.org/10.2147/OTT.S160802

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Samir Farghaly


Hong-xia Hui,1,* Zhong-wu Hu,2,* Chao Jiang,1 Jian Wu,3 Yong Gao,1 Xiao-wei Wang1

1Department of Medical Oncology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, Jiangsu, People’s Republic of China; 2Department of Thoracic Surgery, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, Jiangsu, People’s Republic of China; 3Department of Pathology, Huai’an First People’s Hospital, Nanjing Medical University, Huai’an, Jiangsu, People’s Republic of China

*These authors contributed equally to this work

Background:
This study aimed to investigate the prognostic power of zinc-finger protein 418 (ZNF418) in gastric cancer (GC) and its potential role in GC development and progression.
Patients and methods: A total of 10 GC patients’ individual plasmas were collected and screened for dysregulated mRNA using human microarray. Among these dysregulated mRNAs, ZNF418 was found to be significantly downregulated in IIIA–IV stage GC patients compared to IA–IIA stage GC patients. Subsequently, the ZNF418 levels were detected by quantitative reverse transcription-polymerase chain reaction in both GC plasmas and tissues in a larger sample, and the association between ZNF418 expression level and clinicopathological features as well as overall survival (OS) of GC patients was further analyzed. Finally, a network of ZNF418 interactions with other molecules was predicated in STRING and GEPIA databases.
Results: Human mRNA microarray was performed to screen for abnormally expressed mRNAs between five IIIA–IV stage GC patients’ plasma and five IA–IIA stage GC patients’ plasma. A total of 662 mRNAs were differentially expressed in the IIIA–IV stage GC plasma vs IA–IIA stage GC plasma among all the candidate mRNAs according to the Student’s t-test. Results showed that a decrease in the ZNF418 expression level was associated with the presence of GC and also with higher tumor–node–metastasis stage and lower OS rates compared with that in adjacent noncancerous tissues. Cox regression analysis results demonstrated that the OS was independently correlated with ZNF418 expression. Finally, the prediction results showed that a total of eight mRNAs might have an interaction with ZNF418 in both STRING and GEPIA databases.
Conclusion: ZNF418 was first identified to be significantly downregulated in GC. Our study indicated that ZNF418 might serve as a novel biomarker for GC and was involved in GC development.

Keywords: plasma, diagnosis, prognosis, biomarker
 

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