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Value of Contrast-Enhanced Ultrasound in the Differential Diagnosis of Focal Splenic Lesions

Authors Yang R, Lu Q, Xu J, Huang J, Gao B, Zhang H, Zhou J, Du L, Yan F

Received 5 January 2021

Accepted for publication 8 March 2021

Published 1 April 2021 Volume 2021:13 Pages 2947—2958

DOI https://doi.org/10.2147/CMAR.S300601

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Professor Harikrishna Nakshatri


Rui Yang,1,2 Qiang Lu,1 Jinshun Xu,1,2 Jiayan Huang,1 Binyang Gao,1,2 Huan Zhang,1,2 Jie Zhou,1,2 Lanxin Du,1 Feng Yan2

1Ultrasound Department, West China Hospital of Sichuan University, Chengdu, Sichuan, People’s Republic of China; 2Laboratory of Ultrasound Imaging, West China Hospital of Sichuan University, Chengdu, Sichuan, People’s Republic of China

Correspondence: Feng Yan
Laboratory of Ultrasound Imaging, West China Hospital of Sichuan University, No. 88 Keyuan Road South, Chengdu, Sichuan, 610041, People’s Republic of China
Tel +86 18980606323
Email [email protected]

Purpose: To identify and validate contrast-enhanced ultrasound (CEUS) features for differentiating malignant from benign splenic lesions.
Patients and Methods: Splenic lesions in 123 patients who underwent conventional ultrasound (B-mode US) and CEUS were included in this study. Two radiologists evaluated the sonograms of B-mode and CEUS. Statistical analysis was performed to identify significant imaging predictors for splenic malignant lesions. Two other radiologists independently reviewed B-mode and CEUS sonograms and diagnosed the lesions based on proposed criteria as 1) benign, 2) probably benign, 3) probably malignant or 4) malignant. The diagnostic efficiency between B-mode US and CEUS was compared.
Results: Common imaging findings of malignant lesions included hypoechoic, ill-defined margin, absence of cystic/necrotic portion, presence of splenomegaly on B-mode US, and hypoenhancement, rapid washout and presence of intralesional vessels on CEUS (P < 0.05). Among them, three independent features were identified using multivariate logistic regression analysis: hypoechoic pattern, hypoenhancement pattern and intralesional vessels. When three of these findings were combined as a predictor for splenic malignant lesions, 22 (55.0%) of 40 malignant splenic lesions were identified with a specificity of 100%. The diagnostic performance of two readers using receiver operating characteristic curve analysis was 0.622 and 0.533, respectively, for B-mode US, which was significantly improved to 0.908 and 0.906 for CEUS (P < 0.001). The degree of other diagnostic efficiency and inter-reader agreement also increased with CEUS compared to B-mode US.
Conclusion: CEUS may provide more useful information than B-mode US and improve the diagnosis efficiency for distinguishing malignant from benign splenic lesions.

Keywords: spleen, splenic diseases, contrast-enhanced ultrasound, conventional ultrasound

Introduction

Focal splenic lesions (FSLs) are uncommon in comparison with those of other abdominal parenchymal organs and are often discovered incidentally. However, the differential diagnosis of FSLs remains challenging to radiologists because of the nonspecific nature of conventional abdominal imaging, with a moderate accuracy of approximately 50%.1,2 Although detection of malignant FSLs is rare, primary or secondary malignant tumors can seriously damage splenic function.3 Histological diagnoses, including splenic biopsy and splenectomy, are rarely accomplished because of severe complications, such as hemorrhage, iatrogenic splenosis and sepsis.3–5 Therefore, the investigation of an alternative option for preoperative imaging diagnosis is of vital importance to avoid unnecessary invasive procedures and to conserve the essential immunological function of the spleen.

With the development of contrast agents, contrast-enhanced ultrasound (CEUS) is strongly recommended by the European Federation of Societies for Ultrasound in Medicine and Biology (EFSUMB) as an imaging method for the detection and characterization of focal lesions in the liver, pancreas, and kidney.6–9 Studies have shown that spleen-specific uptake and high accumulation of the second-generation contrast agent SonoVue enable CEUS to assess in real-time splenic lesions in arterial and parenchymal phases.10,11 Previous studies have suggested that CEUS has additional value in the diagnosis of functional asplenia, accessory spleen and vascular splenic pathology, including infarction and trauma.11–16 However, the CEUS features of FSLs have not yet been extensively and systematically investigated, and the study of differentiating benign and malignant FSLs by CEUS is also rare.1,17–19

In this study, we investigated the imaging features of splenic lesions on conventional ultrasound (B-mode US) and CEUS and further assessed the additional value of CEUS for differentiating malignancy from benignancy.

Patients and Methods

Patients

We collected ultrasound reports for the CEUS examination acquired between January 2011 and December 2019 from the database of our department, and we found 1953 patients using the search terms “spleen” or “splenic”. Figure 1 shows the flowchart of patient selection. Finally, 123 patients (62 men, 61 women; mean age 50.5 years; age range 19–83 years) with a mean focal splenic lesion size of 3.2 cm were included in this study. The standard of reference (SOR) confirmed a total of 83 benign lesions (48 hemangiomas, 7 hamartomas, 7 hematolymphangiomas, 3 lymphangiomas, 2 sclerosing angiomatoid nodular transformation (SANT), 13 splenic infractions and 3 complex cysts) and 40 malignant lesions (16 lymphomas, 22 metastases including 7 colorectal carcinomas, 4 ovarian carcinomas, 3 lung carcinomas, 5 hepatocellular carcinomas, 3 pancreatic carcinomas and 2 unknown type). Of the 123 lesions, 37 were confirmed by surgery (n = 29) or biopsy (n = 8). The remaining 86 lesions were confirmed by CT (n = 75) and/or MRI (n = 11). Of the 123 patients with focal splenic lesions, 65 patients had a single lesion, and 58 patients had multiple lesions (≥2 lesions). In patients with multiple lesions, only the largest lesion was selected for evaluation.

Figure 1 Flow chart of patient enrollment.

Abbreviations: CEUS, contrast-enhanced ultrasound; SOR, standard of reference; SANT, sclerosing angiomatoid nodular transformation.

Standard of Reference

In this study, the most preferred SOR was histopathology by surgery or imaging guided fine-needle biopsy. The alternative method to SOR for the diagnosis of benignancy was based on the typical features of benign splenic lesions without extrasplenic malignancy and changes in lesion size on dynamic enhanced CT or MRI. The alternative method to SOR for the diagnosis of malignancy was to combine representative imaging features of nodular spleen involvement on contrast-enhanced CT and/or MR imaging and/or biopsy-proven malignancy at another extrasplenic site and/or decrease in size after chemotherapy. According to the established CT and MRI criteria,4,20–22 dynamic contrast-enhanced CT or MR images were interpreted by two senior radiologists who had at least 10 years of experience in abdominal CT and MRI.

Ultrasound Examination

All B-mode US and CEUS examinations were performed by ultrasound doctors with certificates of National Health Commission of the People’s Republic China, using an IU22 ultrasound system (Philips Medical Solutions, Mountain View, Calif) with a C5-1 MHz convex or an L9-3 MHz linear probe. A mechanical index of less than 0.1 and pulse inversion harmonic imaging were used for CEUS. Imaging parameters were set constant during the examination, and a dual-screen format with the CEUS image alongside the grayscale image was used to accurately locate the lesion during the procedure. The focal zone was placed below the region of interest, and the selected plane remained unchanged. All patients fasted for at least 8 hours before the examination.

For CEUS, a bolus injection of 2.4 mL of sulfur hexafluoride filled microbubble contrast agent (SonoVueTM; Bracco, Milan, Italy) was administered via a 20-gauge catheter line placed in the antecubital vein, followed by a 5-mL flush of 0.9% sodium chloride solution. The imaging timer was started simultaneously with the completion of the contrast agent injection, and the arteria-phase and parenchymal phase images were obtained 5–30 seconds and 60–300 seconds after injection, respectively. The region of interest, including target lesions and surrounding spleen parenchyma, was imaged continuously for 5 minutes or longer. To minimize motional artifacts, patients were asked to remain still and to try to hold their breath during the examination, particularly during early contrast enhancement. The results of ultrasound examination (images or video recordings) were stored on the hard drive of the ultrasound system and copied to the hard disk of a portable computer for later evaluation.

Imaging Assessment

The following basic demographic data of patients were collected: sex, age, diagnosis of the underlying tumor disease, and the SOR of splenic lesions.

All B-mode and CEUS images were anonymized and randomly displayed in the sequence (a) B-mode, (b) arterial phase and (c) parenchymal phase of CEUS scans. The images were reviewed in two separate review sessions by two experienced radiologists who had at least 10 years of experience in abdominal ultrasound and 5 years of experience with CEUS. If there were any inconsistencies, a consensus was reached through discussion with the third observer. None of the observers were involved in the scanning, and all were blinded to the clinical, histopathological or other imaging results of each case.

For B-mode US assessment, the following imaging findings of each case were recorded: (a) size of the largest lesion (maximum diameter); (b) echogenicity of lesions (hyperechoic; isoechoic, or hypoechoic); (c) number of lesions (single; multiple); (d) margin (smooth and well defined; almost well defined; or rough and ill-defined); and (e) presence of splenomegaly (maximum width or length >12 cm).

For CEUS, the relative hypoechoic pattern of the lesion compared to the surrounding spleen parenchyma was recorded and classified as follows: (a) no enhancement (no enhancement in the arterial and parenchymal phase); (b) arterial hyperenhancement (partial or entire lesions showing iso-/hyperenhancement in the arterial phase, with slow and incomplete washout in the parenchymal phase); (c) hypoenhancement (although the degree of enhancement in arterial phase was different, the enhancement in parenchymal phase was significantly lower than that in normal spleen); and (d) delayed hyperenhancement (hypoenhancement in the arterial phase, with partial or entire iso-/hyperenhancement in the parenchymal phase). In addition, other features on CEUS of each lesion were also recorded and characterized: (a) shape after enhancement (regular, almost regular, irregular, wedge shaped); (b) presence or absence of cystic/necrotic portion; and (c) presence or absence of irregular intralesional vessels.

According to the collected data, two other radiologists independently reviewed B-mode and CEUS sonograms and diagnosed the lesions as (1) definitively benign, (2) probably benign, (3) probably malignant or (4) definitively malignant. The diagnostic efficiency in terms of accuracy, sensitivity, specificity, positive and negative predictive values, and area under the receiver operating characteristic curve (AUC) of B-mode US and CEUS was compared.

Statistical Analysis

All statistical analyses were performed using commercially available statistical software (SPSS for Mac, version 26.0; SPSS, Chicago, IL). For differentiating benign and malignant splenic lesions, the X2 test or Fisher’s exact test was used to compare the frequency of qualitative data; Student’s t-test was used for quantitative analysis and presented as the mean ± standard deviation. To determine the variables associated with the predictive malignancy of splenic lesions, binary logistic regression analysis with stepwise forward variable selection was conducted using univariate analysis (P values for entry and removal were 0.05 and 0.10, respectively).

A receiver operating curve analysis was performed to estimate the diagnostic performance and confidence for correct lesion diagnosis. The value of diagnostic performance was calculated in terms of sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV). Weighted kappa statistics were used to assess the interreader agreement in the diagnostic confidence of the conventional and contrast-enhanced sonograms. The degree of agreement was classified as poor (κ< 0.20), moderate (κ, 0.20 to <0.40), fair (κ, 0.40 to <0.60), good (κ, 0.60 to <0.80), or pretty good (κ, 0.80–1.00). Statistical significance was indicated by P value less than 0.05.

Results

The characteristics of patients and imaging findings between malignant and benign splenic lesions on B-mode and CEUS are given in Table 1. For B-mode US, there were significant differences in lesion echogenicity and splenomegaly between malignant and benign cases (P < 0.05). Most malignancies appeared hypoechoic (35/40, 87.5%), while benign lesions mainly appeared hyperechoic (35/83, 48.2%). Compared with benign lesions, malignant tumors more frequently showed splenomegaly (23/40, 57.5%). For CEUS images, malignant splenic lesions more frequently displayed a hypoenhanced pattern (39/40, 97.5%). To be specific, the enhancement of most lesions was substantially lower than that of the spleen in the parenchymal phase followed by rapid and complete washout (Figures 2 and 3). The patterns of CEUS with no enhancement and delayed hyperenhancement only appeared in benign lesions. The hyperenhanced patterns including arterial hyperenhancement and delayed hyperenhancement were mainly observed in benign splenic lesions (Figure 4). All splenic infractions and complex cysts were shown to be completely nonenhanced with a diagnostic accuracy of 100%. The percentage of hypoenhancement pattern was significantly lower in the benign group than in the malignant group (21/83 vs 39/40, P < 0.001). Furthermore, malignant splenic lesions more commonly showed the presence of cystic or necrotic portions (P = 0.033) and intralesional vessels (P < 0.001).

Table 1 Characteristics of Patients and Imaging Findings

Figure 2 Splenic metastasis in a 53-year-old female patient. (A) B-mode US shows a normal-sized spleen with 4.5 cm in diameter inhomogeneous hypoechoic lesion (arrow) with almost regular shape. (B) CEUS shows heterogeneously iso-enhancing (arrow) lesion with some small nonenhanced areas (23 seconds after contrast injection). (C) CEUS shows hypoenhanced lesion with dotted aspect (arrow), followed by rapid and complete wash out in parenchymal phase (97 seconds after contrast injection). (D) Photograph of corresponding pathologic specimen through hematoxylin-eosin staining (100 magnification).

Figure 3 Splenic non-Hodgkin’s lymphoma in a 41-year-old female patient. (A) B-mode US shows a normal-sized spleen with a 4.2 cm in diameter inhomogeneous hypoechoic lesion (arrow). (B) CEUS shows heterogeneously iso/hyperenhanced lesion (arrow) with irregular vessels in arterial phase (15 seconds after contrast injection). (C) CEUS shows the enhancement (arrow) in parenchymal phase was lower than that in normal spleen in parenchymal phase (3 minutes after contrast injection). (D) Photograph of corresponding pathologic specimen through hematoxylin-eosin staining (40 magnification).

Figure 4 Splenic benign vascular tumor in a 48-year-old female patient. (A) B-mode US shows a normal-sized spleen with a 2.4 cm in diameter almost well-defined hypo-echoic lesion (arrow). (B) CEUS shows hyper-enhancing (arrow) in arterial phase (17 seconds after contrast injection). (C) CEUS shows sustained hyper/iso-enhancing (arrow) in parenchymal phase (105 seconds after contrast injection).

Table 2 summarizes the characteristic features predicting malignant focal splenic lesions. Three independent features were identified in the final step of the logistic regression analysis: hypoechoic pattern (odds ratio [OR] = 10.791; 95% confidence interval [CI] = 2.465, 47.239; P < 0.001), hypoenhancement pattern (OR = 70.026; 95% CI = 13.550, 213.420; P < 0.001) and the presence of intralesional vessels (OR = 29.030; 95% CI = 2.792, 301.813; P = 0.005). When combining these three features as a predictor for splenic malignant lesions, the sensitivity, specificity, PPV, NPV and accuracy were 22/40 (55.0%), 83/83 (100.0%), 22/22 (100.0%), 83/101 (82.2%), and 105/123 (85.4%), respectively.

Table 2 Important Features for Prediction of Malignant Focal Splenic Lesions

Based on the analyses of the above imaging characteristics and previous studies,17–19 we proposed the criteria for the prediction of benign and malignant splenic lesions (Table 3). Table 4 summarizes the diagnostic results of other two readers for the 83 benign and 40 malignant splenic lesions based on B-mode US and CEUS criteria. For both readers, the rate of definite diagnosis of benign or malignant splenic lesions for B-mode US was less than 10%, while the rate was significantly increased after reviewing CEUS sonograms (from 10.8% and 8.4% to 60.2% and 61.4% for benign lesions and from 17.5% and 12.5% to 65.0% and 65.0% for malignant lesions, respectively; P < 0.05). In addition, the interreader agreement was improved after reviewing CEUS (weighted κ = 0.53 for B-mode versus 0.78 for CEUS).

Table 3 Diagnostic Criteria for Benign and Malignant Splenic Lesions

Table 4 Diagnostic Results of 123 Splenic Lesions on B-Mode Ultrasound and Contrast-Enhanced Ultrasound n (%)

Table 5 presents the diagnostic efficiency of the two readers for differentiating benign and malignant lesions based on B-mode US and CEUS. Figure 5 shows the individual improvement in diagnostic confidence using receiver operating curve analysis. Compared with B-mode US, CEUS significantly improved the diagnostic performance (overall accuracy 58.5% and 52.0% versus 87.0% and 85.4%, respectively; P < 0.001) and the diagnostic confidence (AUC values 0.622 and 0.533 before versus 0.908 and 0.906 after CEUS for both readers, respectively; P < 0.05) for correct diagnosis of benignancy or malignancy.

Table 5 Diagnostic Efficiency of B-Mode Ultrasound and Contrast-Enhanced Ultrasound Between Benign and Malignant Splenic Lesions

Figure 5 Receiver operating characteristic curves for differentiating benign and malignant splenic lesions after review of B-mode (continuous line) and CEUS (dotted line) sonograms. For reader 1 (A), the diagnostic confidence increased from 0.622 with B-mode US to 0.908 with CEUS and for reader 2 (B), the diagnostic confidence increased from 0.533 with B-mode to 0.906 with CEUS. The improvement in diagnostic confidence after review of CEUS was statistically significant (p < 0.001) for both readers.

Discussion

With the advent of CEUS and second-generation contrast agents (such as SonoVueTM), the diagnostic efficiency of ultrasound in abdominal examination has been significantly improved, especially for most liver lesions, and its sensitivity and specificity are similar to those of CT23 or MRI.24 Moreover, emerging data demonstrated that CEUS can be safely applied in pregnant patients with parenchymal lesions and may add pivotal diagnostic value.25,26 Supplementary to B-mode US, CEUS can provide more microvascular information in lesions and make the contour and shape of lesions clearer. SonoVue, made of sulfur hexafluoride gas microbubbles, is a pure blood pool contrast agent that is specifically captured by spleen macrophages and does not disperse into the interstitial space.10,27 CEUS can be used to differentiate various benign and malignant splenic lesions and to detect traumatic splenic lesions.13,14 However, due to the low occurrence of diseases, the number of CEUS studies is scarce, and the diagnostic criteria of splenic lesions are not clearly defined.

In this study, we enrolled 123 patients who underwent B-mode US and CEUS, and the imaging features of each method were compared in splenic lesions to further assess the value of CEUS in splenic lesion diagnosis. Our results indicated that CEUS not only enhances the diagnostic efficiency of detecting splenic benign and malignant lesions but also improves the reliability and repeatability of ultrasound examination. Previous studies reported that most splenic masses are benign and malignant tumors account for only 20–35%,28,29 which is consistent with our results (40/123, 32.5%; Table 1). Among 83 benign lesions, 13 infarctions and 3 complex cysts presented nonenhancement throughout the CEUS examination. The other 67 lesions displayed various hypoechoic patterns, and most of the lesions were followed by slow and incomplete washout. For malignant lesions, almost all lesions showed hypoenhanced in the parenchymal phase, regardless of the degree of enhancement in the arterial phase, and the contrast washout rate was faster than that of surrounding normal spleen tissues. These findings were in accordance with previous studies that indicated a lower microbubble uptake in malignant splenic lesions than in splenic benign vascular neoplasms such as hemangiomas and/or hamartomas.1,3,15,19,30 The absence of microbubble uptake may also allow for differentiating splenic infarcts12,30–32 as well as avascular cystic parts from solid splenic tumors.11,30

Most splenic lesions are detected incidentally on imaging findings, and the common types of benign lesions are vascular neoplasms, such as hemangiomas and hamartomas.22 Several studies15–17,33 demonstrated that benign vascular neoplasms typically presented initially iso-/hyperenhancement in the arterial phase followed by slow or incomplete washout. Our results corroborate these findings, showing that most hyperenhanced nodules are benign splenic lesions (32/33). Splenic hemangiomas, arising from the sinusoidal epithelium or cavernous vascular channels, have three typical features, including vascularity, sinusoidal reticuloendothelial system cells (RES), and heterogeneity imaging presentation.34 The higher enhancement in the arterial phase is likely due to hypervascular structure with increased arterial supply,22,35,36 while no or minimal washout was observed in the parenchymal phase, showing spleen-like retention of microbubbles.10,20

Splenic malignant tumors are relatively uncommon, and knowledge of malignancy is crucial for further therapeutic decision making. Due to the lack of sinusoidal spaces and RES cells in malignant lesions,18,19 the uptake and retention of microbubbles are significantly lower than those of benign splenic lesions, and the detection rate could be as high as 87%.19 Therefore, hypoenhancement in the parenchymal phase of CEUS may be an important feature in the differentiation of malignant tumors. However, our results show that 21/83 (25.3%) benign lesions had a similar pattern of hypoenhancement. A few reasons can be considered: hypoenhancement may indicate perfused solid splenic lesions of tissue devoid of sinusoids19 and lower microbubble uptake in hypoechoic splenic hemangiomas.1,10,16,17 Taibbi et al37 reported that 8 of 27 hemangiomas showed some degree of enhancement on CEUS but remained substantially lower than that of the surrounding splenic tissue. Stang et al19 reported that the most apparent overlap between benign and malignant splenic lesions was seen with nodular sarcoidosis and lymphoma, both of which were characterized by multiple progressive hypo-enhanced lesions up to 3 cm in diameter. In our study, we found that most of the enrolled hyperechoic benign vascular neoplasms and SANTs showed this pattern. While most malignant lesions were hypoenhanced in the parenchymal phase, the lesions manifesting with this pattern might not necessarily be malignant. Therefore, hypoenhancement alone is not sufficient to determine the malignancy, and other imaging features of CEUS should be considered, such as rapid and complete washout in the parenchymal phase, irregular intralesional vessels, and dotted aspects. These features have also been observed by other research groups.11,19,38 Therefore, in order to better distinguish between benign and malignant splenic lesions, we proposed prediction criteria based on the characteristics of B-ultrasound and contrast-enhanced ultrasound (Table 3). Our results indicated that these criteria were helpful for the differential diagnosis of benign and malignant spleen lesions in this study.

Our study has several limitations. Firstly, not all splenic lesions were confirmed by histopathological examinations. For ethical reasons, biopsies were not systematically performed in patients with lesions that were evaluated as benign based on available imaging data in combination with clinical information. Secondly, compared with benign splenic lesions, the number of malignant splenic lesions was substantially insufficient. Finally, this study only focused on ultrasound imaging modality, while the application of fusion imaging (CEUS plus CT/MRI) may help to elucidate indeterminate parenchymal lesions and further improve the accuracy of diagnosis.39–41 Moreover, fusion imaging could be applicated in numerous fields, particularly in the characterization of parenchymal lesions as well as during interventions.39 Ewertsen et al42 reported that fusion imaging could help to characterize unclear lesions and clarify the benign or malignant status of a greater number of lesions. The application of fusion imaging in splenic lesions will be investigated in our further studies.

Conclusion

In conclusion, CEUS may provide more useful information than conventional ultrasound and significantly improve the diagnostic efficiency for distinguishing malignant from benign splenic lesions. Further optimizing the diagnostic criteria will make CEUS an effective alternative to CT or MRI and reduce unnecessary histological evaluation in clinical scenarios.

Ethics

This is a single-center study and was approved by West China Hospital of Sichuan University Biomedical Research Ethics Committee. Written informed consent was waived because of the retrospective nature of clinical and imaging data collection. The study was conducted in accordance with the Declaration of Helsinki and the privacy of the participants data were protected.

Acknowledgments

We greatly appreciate the financial supports by the 1.3.5 project for disciplines of excellence, West China Hospital, Sichuan University (ZYJC18008), National Science Foundation of China (NSFC) (81701797), Beijing Natural Science Foundation (7192200), Science and Technology Innovation Talent of Sichuan (20CXRC0065), Science and Technology Project of Chengdu (2019-YF05-00376-SN), Science and Technology Project of the Health Planning Committee of Sichuan (20PJ011).

Disclosure

The authors report that they have no conflicts of interest related to this work.

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