Back to Journals » International Journal of Nanomedicine » Volume 6

Validity of bioconjugated silica nanoparticles in comparison with direct smear, culture, and polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum specimens

Authors Ekrami A, Samarbaf-Zadeh AR, Khosravi A, Zargar B, Alavi M, Amin M, Kiasat A

Published 4 November 2011 Volume 2011:6 Pages 2729—2735

DOI https://doi.org/10.2147/IJN.S23239

Review by Single-blind

Peer reviewer comments 3

Alireza Ekrami1, Ali Reza Samarbaf-Zadeh2, Azar Khosravi1, Behrooz Zargar3, Mohamad Alavi1, Mansor Amin2, Alireza Kiasat3
1Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, 2Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, 3Department of Chemistry, School of Science, Shahid Chamran University, Ahvaz, Iran

Background: Tuberculosis is a public health problem worldwide, and new easy to perform diagnostic methods with high accuracy are necessary for optimal control of the disease. Recently, fluorescent silica nanoparticles (FSNP) has attracted immense interest for the detection of pathogenic microorganisms. The aim of this study was to detect Mycobacterium tuberculosis in clinical samples using bioconjugated FSNP compared with microscopic examination, polymerase chain reaction (PCR), nested PCR, and culture as the gold standard.
Methods: In total, 152 sputum specimens were obtained from patients who were suspected to have pulmonary tuberculosis. All samples were examined by the four techniques described.
Results: The assay showed 97.1% sensitivity (95% confidence interval [CI] 91–99.2) and 91.35% specificity (CI 78.3–97.1). Furthermore, assays using variable bacterial concentrations indicated that 100 colony forming units/mL of M. tuberculosis could be detected. There were no differences between the results obtained from two types of mouse monoclonal antibody against Hsp-65 and 16 KDa antigens.
Conclusion: We performed this assay in a large number of clinical samples to confirm the diagnostic specificity and sensitivity of the test and can recommend its application for diagnosis of M. tuberculosis. We believe that this method is more convenient for routine diagnosis of M. tuberculosis in sputum and will be more easily applicable in the field, and with sufficient sensitivity.

Keywords: Mycobacterium tuberculosis, fluorescent silica nanoparticles, bioconjugation, IS6110

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]

 

Readers of this article also read:

Uptake and intracellular traffic of siRNA dendriplexes in glioblastoma cells and macrophages

Perez AP, Cosaka ML, Romero EL, Morilla MJ

International Journal of Nanomedicine 2011, 6:2715-2728

Published Date: 4 November 2011

Preparation of polylactide-co-glycolide nanoparticles incorporating celecoxib and their antitumor activity against brain tumor cells

Kim TH, Jeong YI, Jin SG, Pei J, Jung TY, Kim IY, Kang SS, Jung S.

International Journal of Nanomedicine 2011, 6:2621-2631

Published Date: 28 October 2011

Bactericidal activities of woven cotton and nonwoven polypropylene fabrics coated with hydroxyapatite-binding silver/titanium dioxide ceramic nanocomposite "Earth-plus"

Kasuga E, Kawakami Y, Matsumoto T, Hidaka E, Oana K, Ogiwara N, Yamaki D, Sakurada T, Honda  T

International Journal of Nanomedicine 2011, 6:1937-1943

Published Date: 9 September 2011

Evaluation of injectable silica-embedded nanohydroxyapatite bone substitute in a rat tibia defect model

Xu W, Ganz C, Weber U, Adam M, Holzhüter G, Wolter D, Frerich B, Vollmar B, Gerber T

International Journal of Nanomedicine 2011, 6:1543-1552

Published Date: 2 August 2011

New tumor-targeted nanosized delivery carrier for oligonucleotides: characteristics in vitro and in vivo

Zhou T, Jia X, Li H, Wang J, Zhang H, A Y, Zhang ZZ

International Journal of Nanomedicine 2011, 6:1527-1534

Published Date: 22 July 2011

Poly (ε-caprolactone) nanofibrous ring surrounding a polyvinyl alcohol hydrogel for the development of a biocompatible two-part artificial cornea

Bakhshandeh H, Soleimani M, Shah Hosseini S, Hashemi H, Shabani I, Shafiee A, Behesht Nejad AH, Erfan M, Dinarvand R, Atyabi F

International Journal of Nanomedicine 2011, 6:1509-1515

Published Date: 14 July 2011

Zinc oxide nanoparticles as selective killers of proliferating cells

Taccola L, Raffa V, Riggio C, Vittorio O, Iorio MC, Vanacore R, Pietrabissa A, Cuschieri A

International Journal of Nanomedicine 2011, 6:1129-1140

Published Date: 30 May 2011

Study of pharmacokinetics and tissue distribution of liposomal brucine for dermal administration

Yang B-C, Chu Z-F, Zhu S, Wang L-J, Feng Y-H, Li F-H, Liu C-S, Yuan Y

International Journal of Nanomedicine 2011, 6:1109-1116

Published Date: 26 May 2011

Dispersion stability and exothermic properties of DNA-functionalized single-walled carbon nanotubes

Kawaguchi M, Ohno J, Irie A, Fukushima T, Yamazaki J, Nakashima N

International Journal of Nanomedicine 2011, 6:729-736

Published Date: 8 April 2011