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Using the Systems Biology Approach and Molecular Method to Investigate the Role of the Dopaminergic Pathway in Osteoarthritis: A Case Control Study

Authors Sheikhpour M, Eliaspour D, Arabi I, Raeissadat SA, Lari A, Seif Barghi T

Received 12 November 2019

Accepted for publication 30 January 2020

Published 4 March 2020 Volume 2020:15 Pages 321—327

DOI https://doi.org/10.2147/CIA.S238351

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Richard Walker


Mojgan Sheikhpour,1,2 Darioush Eliaspour,3 Iraj Arabi,3 Seyed Ahmad Raeissadat,3 Arezou Lari,4 Tohid Seif Barghi5

1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; 3Shahid Beheshti University of Medical Sciences, Department of Physical Medicine and Rehabilitation, Tehran, Iran; 4Biomedicine Unit, Pasteur Institute of Iran, Tehran, Iran; 5Department of Sports and Exercise Medicine, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Correspondence: Mojgan Sheikhpour; Darioush Eliaspour
Tel +98 9122969712; +98 9125707896
Fax +98 21 64112313
Email m_sheikhpour@pasteur.ac.ir; d.eliaspour@yahoo.com

Objective: Osteoarthritis is the most common type of arthritis and one of the leading causes of job loss and motor disabilities. Recently, the involvement of dopaminergic pathways and dopamine receptor genes has been considered in this disease. Therefore, studying and comparing the expression pattern of these receptor genes can lead to a greater understanding of the pathogenesis of this disease.
Methods: In this research, we used the systems biology approach to investigate the role of the dopaminergic pathway in osteoarthritis. Then the gene expression pattern of dopamine receptor genes was examined in an osteoarthritis patientgroup in comparison with healthy individuals by Real-time PCR method.
Results: The analysis of the transcriptome dataset of osteoarthritis identified some genes in the dopaminergic pathway and the six most important genes in this disease are in the network with a significant relationship to dopamine receptors which differentially expressed compared to health groups. Statistical analysis of the case control study showed a significant difference (P-value< 0.05) in DRD1 and DRD2 family in the patients in comparison to healthy individuals.
Discussion: We attained the significant expression pattern of dopamine receptors in the blood of osteoarthritis patients which could be useful to identify new strategies for the diagnosis, management, or treatment of this disease.

Keywords: osteoarthritis, systems biology, dopamine receptor genes, gene expression

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