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Use of micro-positron emission tomography with 18F-fallypride to measure the levels of dopamine receptor-D2 and 18F-FDG as molecular imaging tracer in the pituitary glands and prolactinomas of Fischer-344 rats

Authors Li P, Gui S, Cao L, Gao H, Bai J, Li C, Zhang Y

Received 8 August 2015

Accepted for publication 24 October 2015

Published 6 April 2016 Volume 2016:9 Pages 2057—2068

DOI https://doi.org/10.2147/OTT.S94057

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Norbert Ajeawung

Peer reviewer comments 3

Editor who approved publication: Dr Faris Farassati


Ping Li,1–3,* Songbai Gui,2,* Lei Cao,2 Hua Gao,1 Jiwei Bai,2 Chuzhong Li,1 Yazhuo Zhang1

1Beijing Neurosurgical Institute, 2Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, 3Department of Neurosurgery, The Second Affiliated Hospital of Anhui Medical University, Cerebral Vascular Disease Research Center, Anhui Medical University, Hefei, People’s Republic of China

*These authors contributed equally to this work

Abstract: Dopamine receptor-D2 (DRD2) is the most important drug target in prolactinoma. The aim of this current study was to investigate the role of using micro-positron emission tomography (micro-PET) with 18F-fallypride and 18F-fluorodeoxyglucose (18F-FDG) as molecular imaging tracer in the pituitary glands and prolactinomas of Fischer-344 (F344) rats and detect the difference of the levels of DRD2 in the pituitary glands and prolactinomas of F344 rat prolactinoma models. Female F344 rat prolactinoma models were established by subcutaneous administration of 15 mg 17β-estradiol for 8 weeks. The growth of tumors was monitored by the small-animal magnetic resonance imaging and micro-PET. A series of molecular biological experiments were also performed 4 and 6 weeks after pump implantation. The micro-PET molecular imaging with 18F-fallypride revealed a decreased expression of DRD2 in F344 rat prolactinoma models, but the micro-PET molecular imaging with 18F-FDG presented an increased uptake in the prolactinoma compared with the pituitary gland. A decreasing trend of levels of DRD2 in F344 rat prolactinoma models was also detected by molecular biological experiments. From this, we can conclude that micro-PET with 18F-fallypride and 18F-FDG can be used to assess tumorigenesis of the prolactinomas in vivo and molecular imaging detection of DRD2 level in prolactinoma may be an indication of treatment effect in the animal experiment.

Keywords: pituitary prolactin, dopamine agonists, prolactinoma

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