Back to Journals » International Journal of Nanomedicine » Volume 12

Use of magnetic nanoparticles as a drug delivery system to improve chlorhexidine antimicrobial activity

Authors Tokajuk G, Niemirowicz K, Deptuła P, Piktel E, Cieśluk M, Wilczewska AZ, Dąbrowski JR, Bucki R

Received 28 April 2017

Accepted for publication 28 June 2017

Published 25 October 2017 Volume 2017:12 Pages 7833—7846


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Thomas Webster

Grażyna Tokajuk,1,2 Katarzyna Niemirowicz,1 Piotr Deptuła,1,3 Ewelina Piktel,1 Mateusz Cieśluk,1 Agnieszka Z Wilczewska,4 Jan R Dąbrowski,3 Robert Bucki1

1Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, 2Department of Intergrated Dentistry, Medical University of Białystok, 3Department of Materials and Biomedical Engineering, Białystok University of Technology, 4Institute of Chemistry, University of Białystok, Białystok, Poland

Abstract: Nanotechnology offers new tools for developing therapies to prevent and treat oral infections, particularly biofilm-dependent disorders, such as dental plaques and endodontic and periodontal diseases. Chlorhexidine (CHX) is a well-characterized antiseptic agent used in dentistry with broad spectrum activity. However, its application is limited due to inactivation in body fluid and cytotoxicity toward human cells, particularly at high concentrations. To overcome these limitations, we synthesized nanosystems composed of aminosilane-coated magnetic nanoparticles functionalized with chlorhexidine (MNP@CHX). In the presence of human saliva, MNPs@CHX displayed significantly greater bactericidal and fungicidal activity against planktonic and biofilm-forming microorganisms than free CHX. In addition, CHX attached to MNPs has an increased ability to restrict the growth of mixed-species biofilms compared to free CHX. The observed depolarization of mitochondria in fungal cells treated with MNP@CHX suggests that induction of oxidative stress and oxidation of fungal structures may be a part of the mechanism responsible for pathogen killing. Nanoparticles functionalized by CHX did not affect host cell proliferation or their ability to release the proinflammatory cytokine, IL-8. The use of MNPs as a carrier of CHX has great potential for the development of antiseptic nanosystems.

Keywords: chlorhexidine, magnetic nanoparticles, antimicrobial properties, anti-biofilm

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]