Urinary Microbiome Evaluation in Patients Presenting with Hematuria with a Focus on Exposure to Tobacco Smoke
Authors Moynihan M, Sullivan T, Provenzano K, Rieger-Christ K
Received 4 October 2019
Accepted for publication 11 December 2019
Published 27 December 2019 Volume 2019:11 Pages 359—367
Checked for plagiarism Yes
Review by Single-blind
Peer reviewer comments 2
Editor who approved publication: Dr Jan Colli
Matthew Moynihan,1 Travis Sullivan,2 Kacey Provenzano,3 Kimberly Rieger-Christ2
1Department of Surgery, Division of Urology, Lahey Hospital & Medical Center, Burlington, MA 01805, USA; 2Cell and Molecular Biology Laboratory, Lahey Hospital & Medical Center, Burlington, MA 01805, USA; 3Lahey Hospital & Medical Center, Burlington, MA 01805, USA
Correspondence: Matthew Moynihan
Department of Surgery, Division of Urology, Lahey Hospital & Medical Center, 41 Mall Road, Burlington, MA 01805, USA
Purpose: To better characterize the urinary microbiome in males and contribute to overall understanding of the urinary microbiota specifically in patients undergoing evaluation for possible bladder cancer, stratified by risk exposure to smoking.
Patients and Methods: Recruitment of 43 male patients in a sequential manner presenting for hematuria evaluation to a single institution was undertaken. Mid-stream urine specimen pellets were processed through a DNA isolation protocol before undergoing PCR amplification, purification, and 16S rRNA gene sequencing. Gene sequences were clustered into operational taxonomic units and statistical analysis was performed to determine specimen diversity and phylogenetic trends.
Results: No significant difference in microbial diversity was found between the specimens. On subgroup analysis, no significant difference was observed when stratified by either tobacco smoking history or by newly diagnosed urothelial bladder cancer. Variation in microbial diversity was seen amongst all analyzed specimens.
Conclusion: The results of our analysis of carefully selected subjects help to better characterize the urinary microbiome in males and supplements the limited available information on the interrelationship between the urinary microbiome and development of genitourinary malignancy. No significant difference was observed in our small sample size when stratified by tobacco exposure or newly diagnosed bladder cancer.
Keywords: urinary microbiome, tobacco smoking, urothelial carcinoma, hematuria
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]