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Urinary Microbiome Evaluation in Patients Presenting with Hematuria with a Focus on Exposure to Tobacco Smoke

Authors Moynihan M, Sullivan T, Provenzano K, Rieger-Christ K

Received 4 October 2019

Accepted for publication 11 December 2019

Published 27 December 2019 Volume 2019:11 Pages 359—367

DOI https://doi.org/10.2147/RRU.S233386

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 2

Editor who approved publication: Dr Jan Colli


Matthew Moynihan,1 Travis Sullivan,2 Kacey Provenzano,3 Kimberly Rieger-Christ2

1Department of Surgery, Division of Urology, Lahey Hospital & Medical Center, Burlington, MA 01805, USA; 2Cell and Molecular Biology Laboratory, Lahey Hospital & Medical Center, Burlington, MA 01805, USA; 3Lahey Hospital & Medical Center, Burlington, MA 01805, USA

Correspondence: Matthew Moynihan
Department of Surgery, Division of Urology, Lahey Hospital & Medical Center, 41 Mall Road, Burlington, MA 01805, USA
Tel +1781-504-9397
Email Matthew.moynihan@lahey.org

Purpose: To better characterize the urinary microbiome in males and contribute to overall understanding of the urinary microbiota specifically in patients undergoing evaluation for possible bladder cancer, stratified by risk exposure to smoking.
Patients and Methods: Recruitment of 43 male patients in a sequential manner presenting for hematuria evaluation to a single institution was undertaken. Mid-stream urine specimen pellets were processed through a DNA isolation protocol before undergoing PCR amplification, purification, and 16S rRNA gene sequencing. Gene sequences were clustered into operational taxonomic units and statistical analysis was performed to determine specimen diversity and phylogenetic trends.
Results: No significant difference in microbial diversity was found between the specimens. On subgroup analysis, no significant difference was observed when stratified by either tobacco smoking history or by newly diagnosed urothelial bladder cancer. Variation in microbial diversity was seen amongst all analyzed specimens.
Conclusion: The results of our analysis of carefully selected subjects help to better characterize the urinary microbiome in males and supplements the limited available information on the interrelationship between the urinary microbiome and development of genitourinary malignancy. No significant difference was observed in our small sample size when stratified by tobacco exposure or newly diagnosed bladder cancer.

Keywords: urinary microbiome, tobacco smoking, urothelial carcinoma, hematuria

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