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Urinary Cytochrome C and Caspase-3 as Novel Biomarker of Renal Function Impairment in Unilateral Ureteropelvic Junction Obstruction Model of Wistar Rats

Authors Sibarani J, Tjahjodjati T, Atik N, Rachmadi D, Mustafa A

Received 24 April 2020

Accepted for publication 18 June 2020

Published 3 July 2020 Volume 2020:12 Pages 217—224

DOI https://doi.org/10.2147/RRU.S259237

Checked for plagiarism Yes

Review by Single-blind

Peer reviewer comments 4

Editor who approved publication: Dr Jan Colli


Jupiter Sibarani,1 Tjahjodjati Tjahjodjati,1 Nur Atik,2 Dedi Rachmadi,3 Akhmad Mustafa1

1Department of Urology, Faculty of Medicine Universitas Padjadjaran, Hasan Sadikin Hospital Bandung, Bandung, Indonesia; 2Department of Biomedical Sciences, Faculty of Medicine Universitas Padjadjaran, Hasan Sadikin Hospital Bandung, Bandung, Indonesia; 3Department of Pediatric, Faculty of Medicine Universitas Padjadjaran, Hasan Sadikin Hospital Bandung, Bandung, Indonesia

Correspondence: Akhmad Mustafa Jl. Eijkman no. 38, Bandung, Indonesia
Email mustafa.urologi@gmail.com

Introduction: Prolonged obstruction in UPJO would lead to kidney destruction. It is important to find a non-invasive biomarker for early detection of renal impairment before definitive treatment for UPJO. In this study, we aim to evaluate the role of urinary cytochrome c and caspase-3 as a novel biomarker to predict renal function impairment in a UPJO model in Wistar rats.
Methods: Twenty-five male Wistar rats were separated into 3 groups. Group I consists of 5 rats without any treatment or model, group II consists of 5 rats (sham group), and group III consists of 15 rats with the unilateral partial ureteral obstruction model. After 4, 15, and 21 days of observation, the urine was collected and rats were sacrificed to collect the glomerular count in the kidney. Measurement of cytochrome c and caspase-3 was done using the ELISA method, while the glomerular count was done using a light microscope. Data were analyzed using factorial repeated measures ANOVA test and correlation test with Pearson and processed using SPSS version 20.0.
Results: The UPJO group has a significant increase in cytochrome c concentration and caspase-3 concentration compared to the control group and sham group (p< 0.05). There was a significant decrease in the normal glomerulus count of the UPJO group (p< 0.05). There was a significant relationship between the decrease in glomerulus count with the concentration of cytochrome c and caspase-3 in the UPJO group.
Conclusion: There was a significant relationship between the decrease in glomerulus count with the increase in the concentration of cytochrome c and caspase-3 in the UPJO group.

Keywords: ureteropelvic junction obstruction, cytochrome c, caspase-3

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