Upregulation of nucleostemin in colorectal cancer and its effects on cell malignancy
Authors Wei B, Huang Q, Zhong X, Zhang Z
Received 1 December 2014
Accepted for publication 24 April 2015
Published 21 July 2015 Volume 2015:8 Pages 1805—1814
Checked for plagiarism Yes
Review by Single anonymous peer review
Peer reviewer comments 7
Editor who approved publication: Professor Daniele Santini
This paper has been retracted
Bin Wei,1,* Qiaoying Huang,2,* Xiaogang Zhong3
1Department of Gastroenterology and Peripheral Vascular Surgery, The People’s Hospital of Guangxi Zhuang Autonomous Region, 2Department of Medical Molecular Biology, The First Affiliated Hospital of Guangxi University of Chinese Medicine Research, 3Department of Gastroenterology and Peripheral Vascular Surgery, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, People’s Republic of China
*These authors contributed equally to this work
Objective: Nucleostemin (NS) is a new protein localized in the nucleolus of most stem cells and tumor cells, which regulates their self-renewal and cell cycle progression. The aim of this study was to investigate the expression of NS in colorectal cancer (CRC) and the effects of NS knockdown in the Sw620 cell line to provide basis for clinical target therapy.
Methods: NS expression in 372 patients with CRC and 367 normal participants was assessed using immunohistochemistry. The expression level of NS gene was evaluated by polymerase chain reaction. Then, the relationship among NS expression, clinicopathological features, and prognosis was analyzed. Silencing of NS expression was achieved by using NS-specific small-interfering RNAs. The viability and growth rate of Sw620 cells were determined by proliferation and invasion assays. Cell cycle distribution of the cells was analyzed by flow cytometry.
Results: High NS expression was positively related with node metastasis, distant metastasis, and TNM stage. In Kaplan–Meier survival analysis, patients with low NS expression always had significantly longer survival time than those with high expression. Moreover, our results showed that knockdown of NS expression inhibited proliferation and viability of Sw620 cells in a time-dependent manner. Cell cycle studies revealed that NS depletion resulted in G1 cell cycle arrest at short times of transfection (24 hours), followed with apoptosis at longer times (48 hours and 72 hours), suggesting that post-G1 arrest apoptosis occurred in Sw620 cells.
Conclusion: Overall, these results point to the essential role of NS in Sw620 cells; thus, this gene might be considered a promising target for treatment of CRC.
Keywords: apoptosis, colorectal cancer, nucleostemin, small interfering RNA, Sw620, target therapy
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