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Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis

Authors Pan G, Hu T, Chen X, Zhang C

Received 6 August 2019

Accepted for publication 5 October 2019

Published 29 October 2019 Volume 2019:11 Pages 9225—9231

DOI https://doi.org/10.2147/CMAR.S226264

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Colin Mak

Peer reviewer comments 2

Editor who approved publication: Dr Teng


Guangjie Pan,1 Ting Hu,1 Xuewu Chen,1 Chao Zhang2

1Department of Orthopedics, Wenzhou Central Hospital, Wenzhou 325000, People’s Republic of China; 2Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’s Republic of China

Correspondence: Chao Zhang
Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Ouhai District, Wenzhou 325000, People’s Republic of China
Tel +86 15958710270
Email chao_zhao_z@sina.com

Background: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression.
Methods: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay.
Results: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway.
Conclusion: Our findings provide a novel insight about how circRNA regulates OS progression.

Keywords: circMMP9, miR-1265, CHI3L1, osteosarcoma, progression

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