Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
Authors Pan G, Hu T, Chen X, Zhang C
Received 6 August 2019
Accepted for publication 5 October 2019
Published 29 October 2019 Volume 2019:11 Pages 9225—9231
Checked for plagiarism Yes
Review by Single-blind
Peer reviewers approved by Dr Colin Mak
Peer reviewer comments 2
Editor who approved publication: Dr Teng
Guangjie Pan,1 Ting Hu,1 Xuewu Chen,1 Chao Zhang2
1Department of Orthopedics, Wenzhou Central Hospital, Wenzhou 325000, People’s Republic of China; 2Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’s Republic of China
Correspondence: Chao Zhang
Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Ouhai District, Wenzhou 325000, People’s Republic of China
Tel +86 15958710270
Background: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression.
Methods: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay.
Results: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway.
Conclusion: Our findings provide a novel insight about how circRNA regulates OS progression.
Keywords: circMMP9, miR-1265, CHI3L1, osteosarcoma, progression
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.Download Article [PDF] View Full Text [HTML][Machine readable]