Back to Journals » Clinical Ophthalmology » Volume 7

Under-air staining of the anterior capsule using Trypan blue with a 30 G needle

Authors Giammaria D, Giannotti M, Scopelliti A, Pellegrini G, Giannotti B

Received 15 November 2012

Accepted for publication 28 December 2012

Published 29 January 2013 Volume 2013:7 Pages 233—235


Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 5

Daniele Giammaria,1 Michele Giannotti,2 Angelo Scopelliti,1 Giacomo Pellegrini,1 Bruno Giannotti1

Azienda Ospedaliera Ospedali Riuniti Marche Nord, Fano, Italy; 2Catholic University of Rome, Rome, Italy

Abstract: The original technique of staining the anterior capsule of the lens with Trypan blue involves the injection of an air bubble in the anterior chamber. A drawback of this technique is the possible instability of the anterior chamber caused by the sudden exit of air when the dye is injected with the cannula through the side-port incision. Other staining techniques that use viscoelastic substances to increase the stability of the anterior chamber and to dose the injected dye have been described. The authors present an under-air staining technique of the anterior capsule using one drop of Trypan blue injected with a 30 G needle through the peripheral cornea. This procedure prevents the air bubble from escaping the anterior chamber and allows fast and selective staining of the capsule.

Keywords: Trypan blue, staining technique, dye, cataract surgery, capsulorhexis

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]