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Two SNPs in the promoter region of Toll-like receptor 4 gene are not associated with smoking in Saudi Arabia

Authors Kohailan M, Alanazi M, Rouabhia M, Al Amri A, Parine NR, Semlali A

Received 4 May 2016

Accepted for publication 26 July 2016

Published 9 February 2017 Volume 2017:10 Pages 745—752

DOI https://doi.org/10.2147/OTT.S111971

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Ram Prasad

Peer reviewer comments 2

Editor who approved publication: Prof. Dr. Geoffrey Pietersz

Muhammad Kohailan,1 Mohammad Alanazi,1 Mahmoud Rouabhia,2 Abdullah Al Amri,1 Narasimha Reddy Parine,1 Abdelhabib Semlali1

1Department of Biochemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Groupe de Recherche en Écologie Buccale, Département de Stomatologie, Faculté de Médecine Dentaire, Université Laval, Québec, QC, Canada


Abstract: Defects in the innate immune system, particularly in Toll-like receptors (TLRs), have been reported in several cigarette smoke-promoted diseases. The aim of this study was to examine the impact of tobacco smoke on allelic frequencies of TLR4 single-nucleotide polymorphisms (SNPs) and to compare the genotypic distribution of these SNPs in a Saudi Arabian population with that in previously studied populations. DNA was extracted from 303 saliva samples collected from smokers and nonsmokers. Two transitional SNPs in the promoter region of TLR4 were selected, rs2770150 (T/C) and rs10759931 (G/A). Genotype frequencies were determined using quantitative polymerase chain reaction. Our results showed a slight effect of smoking on the distribution of rs2770150 and rs10759931. However, the differences were not significant. Thus, we conclude that the SNPs selected for this study were independent of smoking and may not be related to smoking-induced diseases.

Keywords: Toll-like receptor 4, polymorphism, genetic variation, smoking

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