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Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells

Authors Liu J, Que K, Liu Y, Zang C, Wen J

Received 25 March 2020

Accepted for publication 19 June 2020

Published 6 July 2020 Volume 2020:13 Pages 1655—1664

DOI https://doi.org/10.2147/JPR.S255288

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Michael Schatman


Jie Liu,1,* Kehua Que,1,* Yangqiu Liu,1 Chengcheng Zang,1 Jing Wen1,2

1Department of Endodontics, College of Stomatology, Tianjin Medical University, Tianjin, People’s Republic of China; 2Lotus Dental Clinic, Guangzhou, Guangdong Province, People’s Republic of China

*These authors contributed equally to this work

Correspondence: Jing Wen Tel +86 13682031869
Email www_jing@126.com

Purpose: Transient receptor potential cation channel, subfamily A, member 1 (TRPA1) is a promiscuous chemical nociceptor involved in the perception of cold hypersensitivity, mechanical hyperalgesia and inflammatory pain in human odontoblasts (HODs). Here, we aimed to study the underlying mechanism in which inflammatory cytokine tumor necrosis factor (TNF)-α regulated the expression of TRPA1 channel at both cellular and subcellular levels.
Materials and Methods: Immunohistochemistry was used to confirm the expression of TRPA1 channel in HODs. Dental pulp cells were induced and differentiated to HOD-like cells and used in succedent experiments. Real-time quantitative polymerase chain reaction assay and Western blotting were used to examine the expression changes of TRPA1 channel with the presence and absence of TNF-α and TNF receptor (TNFR) inhibitor, R 7050. Finally, immunoelectron microscopy (IEM) and quantitative analysis were performed to directly display the TNF-α-regulated distribution change of TRPA1 channel in HOD-like cells.
Results: TRPA1 channel was positively expressed in the cell bodies and processes of HODs. The expression TRPA1 channel was significantly up-regulated by high concentration of TNF-α, which could be suppressed by R 7050. Under IEM, TNF-α treatment could increase the expression of TRPA1 in the ER membrane, cytoplasm and mitochondria.
Conclusion: Our study demonstrated that TRPA1 expression in HOD-like cells was evidently upregulated by TNF-α, presumably via TNFR1. TNF-α induced significant increasement in the intracellular distributions of TRPA1 proteins, with increases in the cytoplasm, ER membrane, and mitochondria, to actively participate in noxious external stimuli perception and transduction of hyperalgesia.

Keywords: human odontoblasts, transient receptor potential ankyrin 1, tumor necrosis factor-α, immunoelectron microscopy

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