Back to Journals » Clinical Ophthalmology » Volume 4

Trimetazidine for prevention of induced ischemia and reperfusion of guinea pig retina

Authors Demir T, Turgut B, Ozercan, Gul F, Ilhan N, Çeliker

Published 24 December 2009 Volume 2010:4 Pages 21—26

DOI https://doi.org/10.2147/OPTH.S8409

Review by Single-blind

Peer reviewer comments 4

T Demir1, B Turgut1, I Ozercan2, FC Gul1, N Ilhan3, U Celiker1

1Department of Ophthalmology, 2Department of Pathology, 3Department of Biochemistry, Fırat University School of Medicine, Elazig, Turkey

Objective: Trimetazidine (TMZ) has been used to protect against ischemia/reperfusion (I/R) injury of many tissues. We aimed to evaluate the effect of TMZ during retinal I/R in a guinea pig model.

Study design/patients and methods: An experimental study in retinal I/R. Three groups of five guinea pigs were studied to include a control, placebo, and drug test groups. Prior to the application of 90 minutes of high intraocular pressure (IOP) to induce retinal ischemia followed by 24 hours of reperfusion, we applied intraperitoneal saline to the placebo group and 3 mg/kg of TMZ for the drug test group and repeated the injections at intervals of six hours for four cycles. Both eyes of the animals were enucleated at the end of the reperfusion period. Biochemical assay and histopathologic evaluation was performed on one randomly selected eye of each animal. The level of retinal-free malondialdehyde (MDA) and retinal layer thicknesses were determined and comparisons were then made with the control group.

Results: The mean free MDA level increased in the placebo group (P = 0.006) but not in the drug group (P > 0.05). We observed polymorphonucleated leukocyte infiltration, retinal edema and hydropic degeneration in the retina of the placebo group. However, significant histopathologic change was not observed in specimens of the drug group.

Conclusions: This study suggests TMZ has a beneficial effect on retinal lipid peroxidation and histopathologic changes due to I/R injury.

Keywords: ischemia/reperfusion, retina, trimetazidine

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]