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TRIM28 activates autophagy and promotes cell proliferation in glioblastoma

Authors Peng Y, Zhang M, Jiang Z, Jiang Y

Received 19 September 2018

Accepted for publication 7 December 2018

Published 3 January 2019 Volume 2019:12 Pages 397—404

DOI https://doi.org/10.2147/OTT.S188101

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Andrew Yee

Peer reviewer comments 2

Editor who approved publication: Dr Sanjay Singh


Yong Peng, Mingming Zhang, Zhongzhong Jiang, Yugang Jiang

Department of Neurosurgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China

Background: Tripartite motif-containing protein 28 (TRIM28) is a transcriptional corepressor involved in the regulation of several cancers, including glioma. It has been reported that TRIM28 takes part in the process of autophagy. However, its effect on the autophagy and cell proliferation in gliomas has not been elucidated. Here, we report a novel tumor cell proliferation mechanism in which TRIM28-regulated autophagy promotes glioma tumor cell proliferation.
Materials and methods: We analyzed the expressions of TRIM28 and LC3 in different WHO grades of gliomas by IHC assays. We then knocked down and overexpressed TRIM28 or knocked down ATG5 in U251 cells and confirmed its roles in autophagy and cell proliferation via cell counting, immunofluorescence, and Western blot.
Results: The results showed that TRIM28 and autophagy levels were significantly increased with the progression of tumor grade in glioma. TRIM28 promoted glioblastoma cell proliferation. Knockdown of TRIM28 inhibited autophagy in glioblastoma cells. Meanwhile, TRIM28 promoted glioblastoma cell proliferation by modulating TRIM28.
Conclusion: These data demonstrated that TRIM28 activates autophagy and increases cell proliferation in glioma.

Keywords: TRIM28, autophagy, glioblastoma, proliferation

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