Back to Journals » International Journal of Nanomedicine » Volume 10 » Issue 1

Treatment of a multiple sclerosis animal model by a novel nanodrop formulation of a natural antioxidant

Authors Binyamin O, Larush L, Frid K, Keller G, Friedman-Levi Y, Ovadia H, Abramsky O, Magdassi S, Gabizon R

Received 19 July 2015

Accepted for publication 7 October 2015

Published 20 November 2015 Volume 2015:10(1) Pages 7165—7174


Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Zhengjian Lv

Peer reviewer comments 4

Editor who approved publication: Prof. Dr. Thomas J Webster

Orli Binyamin,1,* Liraz Larush,2,* Kati Frid,1 Guy Keller,1 Yael Friedman-Levi,1 Haim Ovadia,1 Oded Abramsky,1 Shlomo Magdassi,2 Ruth Gabizon1

1Department of Neurology, The Agnes Ginges Center of Human Neurogenetics, Hadassah University Hospital, 2Casali Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel

*These authors contributed equally to this work

Abstract: Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system and is associated with demyelination, neurodegeneration, and sensitivity to oxidative stress. In this work, we administered a nanodroplet formulation of pomegranate seed oil (PSO), denominated Nano-PSO, to mice induced for experimental autoimmune encephalomyelitis (EAE), an established model of MS. PSO comprises high levels of punicic acid, a unique polyunsaturated fatty acid considered as one of the strongest natural antioxidants. We show here that while EAE-induced mice treated with natural PSO presented some reduction in disease burden, this beneficial effect increased significantly when EAE mice were treated with Nano-PSO of specific size nanodroplets at much lower concentrations of the oil. Pathological examinations revealed that Nano-PSO administration dramatically reduced demyelination and oxidation of lipids in the brains of the affected animals, which are hallmarks of this severe neurological disease. We propose that novel formulations of natural antioxidants such as Nano-PSO may be considered for the treatment of patients suffering from demyelinating diseases. On the mechanistic side, our results demonstrate that lipid oxidation may be a seminal feature in both demyelination and neurodegeneration.

Keywords: nanodrops, PSO, EAE, oxidative stress, neurodegeneration

Creative Commons License This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.

Download Article [PDF]  View Full Text [HTML][Machine readable]


Readers of this article also read:

The diameter of nanotubes formed on Ti-6Al-4V alloy controls the adhesion and differentiation of Saos-2 cells

Filova E, Fojt J, Kryslova M, Moravec H, Joska L, Bacakova L

International Journal of Nanomedicine 2015, 10:7145-7163

Published Date: 20 November 2015

Role of surface charge in determining the biological effects of CdSe/ZnS quantum dots

Liu QQ, Li HX, Xia QY, Liu Y, Xiao K

International Journal of Nanomedicine 2015, 10:7073-7088

Published Date: 16 November 2015

Visible-light-responsive ZnCuO nanoparticles: benign photodynamic killers of infectious protozoans

Nadhman A, Nazir S, Khan MI, Ayub A, Muhammad B, Khan M, Shams DF, Yasinzai M

International Journal of Nanomedicine 2015, 10:6891-6903

Published Date: 4 November 2015

Tumor-targeted and pH-controlled delivery of doxorubicin using gold nanorods for lung cancer therapy

Amreddy N, Muralidharan R, Babu A, Mehta M, Johnson EV, Zhao YD, Munshi A, Ramesh R

International Journal of Nanomedicine 2015, 10:6773-6788

Published Date: 29 October 2015

Diagnosis of prostate cancer via nanotechnological approach

Kang BJ, Jeun M, Jang GH, Song SH, Jeong IG, Kim CS, Searson PC, Lee KH

International Journal of Nanomedicine 2015, 10:6555-6569

Published Date: 19 October 2015

Nanoparticles of barium induce apoptosis in human phagocytes

Mores L, França EL, Silva NA, Suchara EA, Honorio-França AC

International Journal of Nanomedicine 2015, 10:6021-6026

Published Date: 28 September 2015

Targeted multidrug-resistance reversal in tumor based on PEG-PLL-PLGA polymer nano drug delivery system

Guo L, Zhang H, Wang F, Liu P, Wang Y, Xia G, Liu R, Li X, Yin H, Jiang H, Chen B

International Journal of Nanomedicine 2015, 10:4535-4547

Published Date: 16 July 2015